Cross‐reactivity among antisera raised against five avian<i>Eimeria</i>species in the natural host and in rabbits

Talebi, Alireza; Mulcahy, Grace

doi:10.1080/03079459508419092

Cited by 11 publications

(7 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The antibody titers from rabbits increased at 14 days PI, peaked at 28 days, and then declined slightly to reach a plateau. This is consistent with previous reports where antibodies persist for prolonged periods in birds and rabbits that have received continuous booster doses [ 7 , 10 , 44 , 45 ]. The pattern of the antibody response indicates a maturation affinity event.…”

Section: Discussionsupporting

confidence: 93%

“…In accordance with Talebi and Mulcahy [ 44 ], the increased titers of antibodies against Eimeria have a significant negative correlation with the oocyst output, Interestingly, we also observed an increase in antibody titers against E. tenella in our immunization/challenge trial. The antibody titers from rabbits increased at 14 days PI, peaked at 28 days, and then declined slightly to reach a plateau.…”

Section: Discussionsupporting

confidence: 92%

“…The pattern of the antibody response indicates a maturation affinity event. It is indicative of effective acquired immunity against E. tenella that would occur immediately after second immunization [ 7 , 10 , 44 , 45 ].…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Protective Immunity Induced by an Eimeria tenella Whole Sporozoite Vaccine Elicits Specific B-Cell Antigens

Juárez-Estrada

Gayosso-Vázquez

Téllez-Isaías

et al. 2021

Animals

View full text Add to dashboard Cite

This study investigated protection against Eimeria tenella following the vaccination of chicks with 5.3 × 106 E. tenella whole-sporozoites emulsified in the nanoparticle adjuvant IMS 1313 N VG Montanide™ (EtSz-IMS1313). One-day-old specific pathogen-free (SPF) chicks were subcutaneously injected in the neck with EtSz-IMS1313 on the 1st and 10th days of age. Acquired immunity was assayed through a challenge with 3 × 104 homologous sporulated oocysts at 21 days of age. The anticoccidial index (ACI) calculated for every group showed the effectiveness of EtSz-IMS1313 as a vaccine with an ACI of 186; the mock-injected control showed an ACI of 18 and the unimmunized, challenged control showed an ACI of −28. In a comparison assay, antibodies from rabbits and SPF birds immunized with EtSz-IMS1313 recognized almost the same polypeptides in the blotting of E. tenella sporozoites and merozoites. However, rabbit antisera showed the clearest recognition pattern. Polypeptides of 120, 105, 94, 70, 38, and 19 kDa from both E. tenella life cycle stages were the most strongly recognized by both animal species. The E. tenella zoite-specific IgG antibodies from the rabbits demonstrated the feasibility for successful B cell antigen identification.

show abstract

Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 92%

See 1 more Smart Citation

Protective Immunity Induced by an Eimeria tenella Whole Sporozoite Vaccine Elicits Specific B-Cell Antigens

Juárez-Estrada

Gayosso-Vázquez

Téllez-Isaías

et al. 2021

Animals

View full text Add to dashboard Cite

show abstract

“…The antibody responses of chickens to Eimeria infections have been characterized with regard to parasite lifecycle stages, immunodominant antigens and dynamics (Xie et al, 1992;Smith et al, 1993;Tomley, 1994;Talebi and Mulcahy, 1995;Tajima et al, 2003;Onaga et al, 2005;Constantinoiu et al, 2007). However, most studies of the antibody responses reported to date have been conducted with wild-type strains that have not been subject to any selection.…”

Section: Introductionmentioning

confidence: 96%

Characterization of the antibody response in birds following infection with wild-type and attenuated strains of Eimeria tenella and Eimeria necatrix

Constantinoiu

Molloy

Jorgensen

et al. 2011

Veterinary Parasitology

View full text Add to dashboard Cite

Live vaccines containing attenuated parasite strains are increasingly used to control chicken coccidiosis. In this paper antibody responses elicited by infections with wild-type and attenuated strains of Eimeria tenella and Eimeria necatrix were characterized by immunoblotting and ELISA with homologous and heterologous antisera. Few differences between antisera from birds infected with wild and attenuated strains of E. tenella were evident in immunoblots conducted with merozoite antigen preparations from both E. tenella strains, however the reactivity of sera raised in birds infected with the wild-type strain was noticeably more intense. In ELISAs conducted with merozoite antigen preparations, antisera from birds infected with the wild-type strains of E. tenella and E. necatrix consistently produced a significantly higher (P<0.05) antibody response than antisera from birds infected with the attenuated strains. Likewise, avidity ELISAs conducted with the E. tenella strains demonstrated that antibodies in birds infected with the wild-type strain were of significantly higher avidity (P<0.05) than antibodies in birds infected with the attenuated strain. The differences in the antibody responses are probably due to changes in the attenuated strain as a result of selection for precocious development and the less severe tissue damage and inflammation of the intestine resulting from infection with the attenuated strain.

show abstract

“…Coccidiosis is one of the most common and problematic parasitic diseases in all classes of domestic animals caused by several species of coccidia (Tolebi and Mulcahy, 1995). It is a ubiquitous protozoan infection of animals seriously impairing their growth and food utilization (Abdel-Megeed et al, 2005) and causes significant mortality in domestic rabbits (Hauptman et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Some heterocyclic thione derivatives exhibit anticoccidial activity by inhibiting glycosidases.

Balbaa

El-Hady²,

Taha³

et al. 2012

Acta Biochim Pol

View full text Add to dashboard Cite

Coccidiosis is one of the most common parasitic diseases affecting many species of domestic animals. This disease has a major economic significance and the search for new compounds having anticoccidial activity is of great importance. In this article, different levels of protection from coccidian infection by Eimeria stiedae were developed in rabbits by treatment with compounds incorporating the skeleton of thiourea. These compounds include 4,5-diphenylimidazole-2-thione (1), 4,5-Diphenyl- 1,2,4-triazole-3-thiol (2) and 5-(2-Hydroxyphenyl)-4-phenyl-1,2,4-triazole-3-thiol (3) compared to the anticoccidial drug toltrazuril as a reference compound. Compounds 1-3 inhibit coccidiosis-induced activity of α-glucosidase.The protection from coccidial infection by compound 1 was higher than that shown for compounds 2 and 3. These data suggest that diazole and triazole thione derivatives have a mimetic effect for anticoccidial drugs through their inhibition of glycosidases.

show abstract

Cross‐reactivity among antisera raised against five avianEimeriaspecies in the natural host and in rabbits

Cited by 11 publications

References 22 publications

Protective Immunity Induced by an Eimeria tenella Whole Sporozoite Vaccine Elicits Specific B-Cell Antigens

Protective Immunity Induced by an Eimeria tenella Whole Sporozoite Vaccine Elicits Specific B-Cell Antigens

Characterization of the antibody response in birds following infection with wild-type and attenuated strains of Eimeria tenella and Eimeria necatrix

Some heterocyclic thione derivatives exhibit anticoccidial activity by inhibiting glycosidases.

Contact Info

Product

Resources

About