Cross-Reaction of Lupus Anti-dsDNA Antibodies with Protein Translation Factor EF-2

Alberdi, Fernando; Dadone, Jorge; Ryazanov, Alexey G.; Isenberg, David; Reichlin, Morris

doi:10.1006/clim.2000.4970

Cited by 17 publications

(13 citation statements)

References 26 publications

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“…The list of proposed immunoproteins here presented includes α-enolase, elongation factor 2 and Glycyl Aminoacyl-tRNA Synthetase. Antibodies versus all the antigens above been reported in patients with various pathologies including rheumatic diseases, myositis and dermatomyositis [25][26][27][28][29][30] but in spite a general consensus in considering these antigens in some way linked to the autoimmune-response several questions remain unresolved. A critical point in defying new auto-antigens in glomerulonephritis is the necessity that they satisfy at least three criteria.…”

Section: Discussionmentioning

confidence: 99%

Direct characterization of target podocyte antigens and auto-antibodies in human membranous glomerulonephritis: Alfa-enolase and borderline antigens

Bruschi

Carnevali

Murtas

et al. 2011

Journal of Proteomics

104

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Section: Discussionmentioning

confidence: 99%

Direct characterization of target podocyte antigens and auto-antibodies in human membranous glomerulonephritis: Alfa-enolase and borderline antigens

Bruschi

Carnevali

Murtas

et al. 2011

Journal of Proteomics

104

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“…These include nucleosomes on human leukocytes, Fc receptors on human T-cells, DNA on mouse and human mononuclear cells, Hp8 on human and murine tubular cells, ribosomal P protein on rat and human glomerular mesangial cells, brush border myosin (110 kDa) on rat hepatoma cells, and some membrane proteins of molecular weights 102, 80, 42, 35 and 31 kDa on a series of human tumor cell lines [36]. Some proteins have been identified as receptors for anti-dsDNA penetrating into live cells.…”

Section: Discussionmentioning

confidence: 99%

Origin and anti-tumor effects of anti-dsDNA autoantibodies in cancer patients and tumor-bearing mice

Zhang

et al. 2005

Immunology Letters

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“…The LPL preparation was characterized by 15% SDS-PAGE, as described previously (11). A complementary DNA encoding the human LPL sequence of 448 amino acids has previously been isolated (12); the calculated molecular weight for the mature protein is 50,394, and with 3 glycosylation sites, a total molecular weight of 60,000 is expected.…”

Section: Methodsmentioning

confidence: 99%

Autoantibodies to lipoprotein lipase and dyslipidemia in systemic lupus erythematosus

Reichlin

Fesmire

Quintero-Del-Rio

et al. 2002

Arthritis & Rheumatism

Self Cite

115

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Objective. To demonstrate the binding of bovine lipoprotein lipase (LPL) by IgG from sera obtained from patients with systemic lupus erythematosus (SLE) and other rheumatic diseases, and the relationship of anti-LPL to triglyceride levels in SLE.Method. Binding of LPL by IgG from sera obtained from patients with SLE and other rheumatic diseases was measured by an enzyme-linked immunosorbent assay technique. Lipid profiles for fasting blood samples obtained from SLE patients and control subjects were determined.Results. Sera obtained from 105 patients with SLE were assessed for reactivity with LPL, and 49 (47%) of the results were positive. Sera obtained from patients with rheumatoid arthritis (RA) (n ‫؍‬ 80), Sjögren's syndrome (n ‫؍‬ 30), polymyositis and dermatomyositis (n ‫؍‬ 30), and progressive systemic sclerosis (n ‫؍‬ 31) were also studied, and 10 (13%), 3 (10%), 12 (40%), and 13 (42%), respectively, were positive for reactivity with LPL. It was determined that all affinity-purified antidouble-stranded DNA (dsDNA) antibodies and 4 of 5 monoclonal anti-dsDNA antibodies bound to LPL. The binding of IgG depleted of anti-dsDNA to LPL indicates a second anti-LPL activity in SLE. Measurements of fasting lipid levels in SLE patients with anti-LPL revealed a strong positive correlation of antibody levels and total serum triglycerides, apolipoprotein B, and apolipoprotein E concentrations.Conclusion. Antibodies to LPL occurred in 47% of SLE patients and in a similar percentage of patients with polymyositis or systemic sclerosis. The prevalence of these antibodies was less in patients with RA or Sjögren's syndrome. It is hypothesized that the elevated triglyceride levels in SLE patients are in part attributable to anti-LPL, and this lipid abnormality could contribute to the premature atherosclerosis known to be present in patients with SLE.

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Cross-Reaction of Lupus Anti-dsDNA Antibodies with Protein Translation Factor EF-2

Cited by 17 publications

References 26 publications

Direct characterization of target podocyte antigens and auto-antibodies in human membranous glomerulonephritis: Alfa-enolase and borderline antigens

Direct characterization of target podocyte antigens and auto-antibodies in human membranous glomerulonephritis: Alfa-enolase and borderline antigens

Origin and anti-tumor effects of anti-dsDNA autoantibodies in cancer patients and tumor-bearing mice

Autoantibodies to lipoprotein lipase and dyslipidemia in systemic lupus erythematosus

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