Cross-reaction between Streptococcus pneumoniae and group C streptococcal latex reagent

Lee, Pik-Chen; Wetherall, B L

doi:10.1128/jcm.25.1.152-153.1987

Cited by 13 publications

(3 citation statements)

References 13 publications

(11 reference statements)

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“…Technical decisions as to what colonial appearances merit picking from culture plates and whether to consider the number of colonies present on the plate may have some influence on the isolates selected for a collection. The cross reactions between group C streptococcal latex reagents and S. pneumoniae and Klebsiella pneumoniae may further lead to misidentification of isolates if they are not tested pure (Lee & Wetherall, 1987;Gordon, Damm & Anderson, 1987). We did not specify the procedures to be used for primary isolation or identification of group C streptococci in the laboratories contributing to this study.…”

Section: Discussionmentioning

confidence: 99%

Group C streptococci in human infection: a study of 308 isolates with clinical correlations

et al. 1989

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A collection of 308 clinical isolates of beta-haemolytic Lancefield group C streptococci was assembled from laboratories in England, Nigeria and New Zealand. Of these, 276 isolates were Streptococcus equisimilis, 23 S. milleri and nine S. zooepidemicus. Isolates of S. equisimilis in the African collection, though few, gave higher rates of lactose and raffinose fermentation, aesculin hydrolysis and positive alpha-galactosidase reactions than those from elsewhere. Erythromycin resistance was found in 1.9% of the English isolates of S. equisimilis. Strains from superficial infections accounted for 88% of the collection and were most commonly isolated from the upper respiratory tract, skin or wounds. Amongst the 36 patients yielding isolates from deep sites S. equisimilis was found in septicaemia, cellulitis, abscess, peritonitis, septic arthritis, pneumonia, mycotic aneurysm and acute epiglottitis, S. milleri was found in abdominal abscesses, peritonitis, pleural empyema and osteomyelitis and S. zooepidemicus was found in septicaemia, pneumonia, meningitis and septic arthritis. Within the collection an unselected general catchment of 214 isolates of group C streptococci from the laboratories in Yorkshire showed the following species: from 199 superficial infections 94% S. equisimilis, 5% S. milleri and 1% S. zooepidemicus and 15 patients with deeper, more aggressive infections 67, 27 and 6.7% of these species respectively.

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Section: Discussionmentioning

confidence: 99%

Group C streptococci in human infection: a study of 308 isolates with clinical correlations

et al. 1989

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“…The streptococcal groups B and D gave no false-positive reactions, and polyagglutination was rare. However, as previously described (8), the group C streptococcal reagent gave many false-positive reactions, and it seems not to be applicable for the rapid identification of streptococci from positive blood culture.…”

Section: Discussionmentioning

confidence: 91%

Rapid immunodiagnosis of streptococci and enterococci in blood cultures^Note

Kristensen¹,

Højbjerg²,

Schønheyder³

2001

APMIS

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We evaluated a procedure for rapid identification of blood culture isolates of pneumococci, enterococci and beta-haemolytic streptococci groups A, B, C, and G. Immunological tests were applied directly to blood culture medium and included a quellung reaction for pneumococci, and latex agglutination tests (LAT) for pneumococcal antigen and Lancefield antigens A, B, C, D and G. During a one-year trial period with approximately 12,000 blood culture sets (BacT/Alert, Organon-Teknika), 208 sets showed pure growth of gram-positive cocci in pairs or chains by direct microscopy. Overall, a correct diagnosis was noted for 103 (63.2%, 95% CI: 55.3-70.6%) of 163 sets yielding pneumococci, beta-haemolytic streptococci, or enterococci. A procedure excluding Lancefield antigens only A, B, and D was implemented during a one-year follow-up period. With this simplified procedure positive and negative predictive values, respectively, were 1.00 and 0.87 for pneumococci, 1.00 and 0.94 for beta-haemolytic streptococci group A, 0.60 and 1.00 for beta-haemolytic streptococci group B, and 0.91 and 0.88 for enterococci. We conclude that rapid identification of gram-positive cocci is feasible and may improve the information given to clinicians at the first notification of positive blood cultures.

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“…There are several possible explanations for the few positive rapid tests which were not confirmed by culture. Antigenic cross-reactions between group C BHS and S. pneumoniae and Klebsiella pneumoniae have been reported by using latex agglutination methods with blood culture and skin swab specimens (9,12,17). False-positive results of the trivalent color test for group C organisms have also been reported for individuals with blood group A (13).…”

mentioning

confidence: 99%

Latex agglutination testing directly from throat swabs for rapid detection of beta-hemolytic streptococci from Lancefield serogroup C

et al. 1992

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A latex agglutination method for the rapid detection of beta-hemolytic streptococci from Lancefield serogroup C in throat swabs from 403 university students with symptomatic pharyngitis was evaluated. Compared with culture, the rapid test was poorly sensitive (34.4%) but very specific (98.4%) in detecting group C beta-hemolytic streptococci. The sensitivity of the rapid test improved with an increasing quantity of growth on culture.

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Cross-reaction between Streptococcus pneumoniae and group C streptococcal latex reagent

Cited by 13 publications

References 13 publications

Group C streptococci in human infection: a study of 308 isolates with clinical correlations

Group C streptococci in human infection: a study of 308 isolates with clinical correlations

Rapid immunodiagnosis of streptococci and enterococci in blood cultures^Note

Latex agglutination testing directly from throat swabs for rapid detection of beta-hemolytic streptococci from Lancefield serogroup C

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Cross-reaction between Streptococcus pneumoniae and group C streptococcal latex reagent

Cited by 13 publications

References 13 publications

Group C streptococci in human infection: a study of 308 isolates with clinical correlations

Group C streptococci in human infection: a study of 308 isolates with clinical correlations

Rapid immunodiagnosis of streptococci and enterococci in blood culturesNote

Latex agglutination testing directly from throat swabs for rapid detection of beta-hemolytic streptococci from Lancefield serogroup C

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Rapid immunodiagnosis of streptococci and enterococci in blood cultures^Note