Cross-reacting and specific antigenic components in cystic fluid from metacestodes of Echinococcus granulosus and Taenia solium

Abstract

Sera from confirmed patients of 5 hydatidosis, 67 neurocysticercosis and 89 other parasitic diseases were tested for specific antibody (IgG) levels by ELISA to cystic fluid antigens from metacestodes of Echinococcus granulosus (HF) and Taenia solium (CF). All hydatidosis sera reacted positively to both HF and CF while neurocysticercosis sera did in 49.3% to HF and 85.1% to CF. The frequencies of cross-reactions were lower in other parasitic diseases to both antigens. By SDS-PAGE, protein bands of 64, 35, 22 and 7 kilodaltons (kDa) were found common in HF and CF. SDS-PAGE/immunoblot exhibited that hydatidosis sera reacted crossly to CF at 135, 110, 100, 86, 64, 45, 39, 35 and 24 kDa bands while neurocysticercosis sera did to HF at 135, 100, 86, 64, 52, 39, 35, 29 and 24 kDa bands. These results indicated that protein bands of 135, 100, 86, 64, 39, 35 and 24 kDa were major common components in HF and CF. Protein bands of 7 kDa in HF and 15, 10 and 7 kDa in CF did not react crossly and were specific components in respective antigens.

Application of Taenia saginata metacestodes as an alternative antigen for the serological diagnosis of human neurocysticercosis

“…These antigens are responsible for the cross-reactivity between the parasites. Kong et al (1989) identified seven immunodominant proteins (135,100,86,64,39,35, and 24 kDa) recognized in individuals infected with E. granulosus with WB using vesicle liquid from T. solium metacestodes. Similarly, five individuals infected by E. granulosus recognized the immunodominant proteins of 64-68, 39-42, and 24 kDa from the same reaction using SE from T. solium metacestodes and T. saginata metacestodes.…”

Novel antigens for neurocysticercosis: simple method for preparation and evaluation for serodiagnosis.

“…3) Cyst fluid had less cross-reactivity with other parasites, especially Echinococcus spp., than intact cysts. 7,8,15,16 Several studies have demonstrated that the 10-26-kD antigen components of cysticerci are the ones most specific for differential serodiagnosis of NCC. 6,8,9 The GPs identified in our study appear to be equivalent to them.…”

Hydrophobic fraction of Taenia saginata metacestodes, rather than hydrophilic fraction, contains immunodominant markers for diagnosing human neurocysticercosis

“…Seven immunodominant proteins (135, 100, 86, 64, 39, 35 and 24kDa) had already been recognized in serum samples from individuals infected with Echinococcus granulosus, in IB using vesicle liquid from Taenia solium metacestodes 37 . In the present study, various proteins from saline antigen were also recognized in patients infected with Echinococcus granulosus, as demonstrated previously by our group 18 in relation to using Taenia saginata metacestodes.…”