Cross-protective efficacy of dendritic cells targeting conserved influenza virus antigen expressed by Lactobacillus plantarum

Abstract

Avian influenza virus (AIV) can infect birds and mammals, including humans, and are thus a serious threat to public health. Vaccination is vital for controlling AIV circulation. In this study, we generated a recombinant lactobacillus expressing the NP-M1-DCpep of H9N2 avian influenza virus and evaluated the activation effect of NC8-pSIP409-NP-M1-DCpep on dendritic cells (DCs) in a mouse model. The specific mucosal antibody responses and B and T cell responses in lymphoid tissues were also characterized. Importantly, we confirmed that specific CD8 T cells presented in vitro and antigen-specific cytotoxicity (activated the expression of CD107a) and in vivo antigen-specific cytotoxicity after vaccination. The adoptive transfer of NC8-pSIP409-NP-M1-DCpep-primed CD8+ T cells into NOD-SCID mice resulted in effective protection against mouse-adapted AIV infection. In addition, we observed protection in immunized mice challenged with mouse-adapted H9N2 AIV and H1N1 influenza virus, as evidenced by reductions in the lung virus titers, improvements in lung pathology, and weight loss and complete survival. Our data are promising for the generation of effective, non-traditional influenza vaccines against AIVs.

Immune response characterization of mice immunized with Lactobacillus plantarum expressing spike antigen of transmissible gastroenteritis virus

“…Dendritic cells are a type of antigen-presenting cell, and they have a significant function in delivering antigens to T cells and B cells to improve the effects of vaccines. Studies conducted by our laboratory and others show that recombined bacteria could trigger the activation of DCs at mucosal sites (Mohamadzadeh et al 2009;Yang et al 2016). In this study, a clear and significant increase in the production of CD11c + CD80 + and CD11c + CD86 + was found in the group immunized with Lp-pSIP-409-17 when compared to the other vaccinated groups.…”

Immune responses induced by recombinant Lactobacillus plantarum expressing the spike protein derived from transmissible gastroenteritis virus in piglets

“…In previous reports, this vaccine strategy offered a variety of benefits in that the constructed L. plantarum expression of protein-fused DCpep could activate mucosal DCs, B cells, and T cells to induce the immunological response and could regulate inflammatory responses that took place in a mucosal microenvironment (Steinman and Idoyaga 2010). Our laboratory has previously studied Yang et al 2016) the effects of recombinant lactic acid bacteria expressing target antigens and DCpep on MHC-II + CD80 + DCs; however, the impact of recombinant lactic acid bacteria cells has not been studied in MHC-II + CD80 + B (such cells not only can secrete SIgA involved in mucosal immunity but also can act as antigen-presenting cells) (Adler et al 2017;Mizoguchi and Bhan 2017). Therefore, in this study, we mainly researched the effects of recombinant lactic acid bacteria NC8-pSIP409-pgsA-S-DCpep expressing DCpep and target antigen S protein on MHC-II + CD80 + B cells, CD3 + CD4 + cells related to mucosal immune responses, and anti-TGEVspecific antibodies.…”

New Progress Regarding the Use of Lactic Acid Bacteria as Live Delivery Vectors, Treatment of Diseases and Induction of Immune Responses in Different Host Species Focusing on Lactobacillus Species

“…The study of the effect of a probiotic supplement on the incidence of upper respiratory tract infections [61], and the metabolism of aromatic amino acids after exhaustive aerobic exercise in trained athletes daily supplementation with probiotics was found to be associated with a lower frequency of URTIs in athletes and seems to be beneficial in increasing training efficacy [79]. Respiratory viral infection via metabolic reprogramming and immune cell modulation by Lactobacillus johnsonii [24], Avian Infuenza infection [34,80,81]. In additional Lactobacillus plantarum GBLP2 isolated from Korean fermented vegetable exhibited preventive effect against the influenza virus infections in mice [82].…”