Cross-Linking and Biodegradation of Native and Denatured Collagen

Tomihata, Kenji; K, Burczak; Shiraki, Kentaro; Ikada, Yoshito

doi:10.1021/bk-1994-0540.ch024

Cited by 57 publications

(52 citation statements)

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“…This biodegradability process depends on the type and concentration of the crosslinking agent, and also on the time and temperature of the crosslinking process. This was also shown by other scientists by using crosslinked gelatins used in biomedical applications (11,12).…”

Section: Introductionsupporting

confidence: 73%

Biodegradation of Chemically Modified Gelatin Films in a Simulated Natural Environment

Далев¹,

Staromanova²,

Dalev³

et al. 2001

Biotechnology & Biotechnological Equipment

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Section: Introductionsupporting

confidence: 73%

Biodegradation of Chemically Modified Gelatin Films in a Simulated Natural Environment

Далев¹,

Staromanova²,

Dalev³

et al. 2001

Biotechnology & Biotechnological Equipment

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“…EDC has the advantages of not being incorporated into the structure of the protein and not being toxic in comparison with cross-linkers such as formaldehyde and glutaraldehyde. 15 Chambi and Grosso evaluated the mechanical properties, morphology and water vapor permeability of gelatin and casein films cross-linked with TGase. 16 A synergistic effect on the Cas/Gel film properties, mainly in a 75:25 (m/m) formulation, was observed by the authors.…”

Section: Introductionmentioning

confidence: 99%

Protein-based films cross-linked with 1-ethyl-3-(3-dimethylamino-propyl) carbodiimide hydrochloride (EDC): effects of the cross-linker and film composition on the permeation rate of p-hydroxyacetanilide as a model drug

Cristiano¹,

Fayad²,

Porto³

et al. 2010

J. Braz. Chem. Soc.

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Filmes reticulados de gelatina (Gel), caseína (Cas) e a mistura Gel/Cas (1:1, m/m) foram estudados em termos das propriedades térmicas, morfológicas e absorção de água e quanto ao perfil de permeação de p-hidroxiacetanilida (p-HAA ) como fármaco modelo. O grau de reticulação por 1-etil-3-(3-cloridrato) dimetilaminopropil carbodiimida (EDC) foi de 90,6% para filmes de Gel e cerca de 70% para filmes de Cas e misturas Gel/Cas (1:1, m/m). A total solubilidade dos filmes não-reticulados foi reduzida para somente 21 -22% após reticulação com EDC. Apesar do alto grau de reticulação, o intumescimento dos filmes de Gel (87,2%) foi praticamente o dobro do determinado para filmes de Gel/Cas (1:1, m/m) e Cas. A taxa de permeação de p-HAA seguiu a ordem Gel > Gel/Cas @ Cas para filmes reticulados, ou seja, um comportamento consistente com o maior intumescimento da Gel em relação aos outros dois sistemas estudados. Os menores valores de permeação observados para filmes de Cas e Gel/Cas, sugerem que estes sistemas podem ser utilizados para controlar a permeação de fármacos. Considerando os diferentes modelos matemáticos aplicados, os sistemas estudados não mostraram um bom ajuste relativamente a equação de primeira ordem e somente uma parcial correlação com as equações de ordem zero e do modelo de Higuchi. No entanto, uma boa correlação (r 2 > 0,99) foi obtida utilizando a equação da lei das potências (valores de n = 0,70 -0,87), indicando que a permeação de p-HAA através dos filmes foi controlada principalmente pelo mecanismo de difusão.Cross-linked films of gelatin (Gel), casein (Cas) and their (1:1, m/m) mixture (Gel/Cas) were studied in terms of their thermal, morphological and water absorption properties and the permeation profile of p-hydroxyacetanilide (p-HAA) as a model drug. The degrees of cross-linking by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) were 90.6% for Gel films and approximately 70% for Cas and Gel/Cas films. The totally soluble non cross-linked films achieved only 21 -22% of solubility after cross-linking with EDC. Despite the high cross-linking degree, the swelling of Gel films (87.2%) was practically twice that determined for Gel/Cas and Cas films. The permeation rate of p-HAA followed the order Gel > Gel/Cas @ Cas for cross-linked films, which is consistent with the Gel film showing a greater swelling than the other two systems studied. Lower permeation rates were observed for Cas and Gel/Cas films, suggesting that these could be used as rate-controlling systems for drug permeation. For the different mathematical models applied, the systems studied did not show a good fit with the first-order equation and only a partial fit with the zero-order equation and Higuchi's model. However, good correlations (r 2 > 0.99) were obtained using the power law equation (n = 0.70 -0.87), indicating that the p-HAA permeation through the films was mainly controlled by a diffusion mechanism.Keywords: gelatin, casein, films, cross-linking, drug permeation Introduction Gelatin (Gel), which is obtain...

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“…According to Fig.5, we knew that utilizing cross-linking method of solution could produce cross-linked HA derivative hydrogel films with water contents as low as 60 wt%, although it took 5 days to obtain such films. It was likely that the optimal pH of the casting solution for HA cross-linked by PEG was around 8, so that the PEG concentration was higher than around 1% [7,8] .…”

Section: Rheology Characteristicmentioning

confidence: 99%

Rheological and biological characteristics of hyaluronic acid derivative modified by polyethylene glycol

Chen

Jingtao

Zheng

2008

J. Wuhan Univ. Technol.-Mat. Sci. Edit.

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Hyaluronic acid (HA) was chemically modified by polyethylene glycol. Meanwhile, the dynamic mechanics properties of HA derivative and its viscoelastic changes were measured on 3ARES3 Rheometer (Japan) at 25 ℃. Dried cross-linked films of 10×10 mm 2 were immersed in phosphate buffered saline(PBS: pH 7.4) at 37 ℃ with different time periods to measure its water content and in vitro degradation. Moreover, cell cultured solutions, which were in the different cultivation vesse with 1 mg/mL Solution of HA derivative as doing experimental sample for 2 d, 4 d and 7 d, were observed, respectively, by an inverted discrepancy microscope. The cell relative growth rate was analyzed with the SPSS10.0 mathematic statistic software. Based on the above experiments, structure-modified HA derivative can meet the requirements of biomaterials in view of rheological and degradation in vitro and cytotoxicity charactereistics from clinical medical aspect under this experiment conditions.

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Cross-Linking and Biodegradation of Native and Denatured Collagen

Cited by 57 publications

References 0 publications

Biodegradation of Chemically Modified Gelatin Films in a Simulated Natural Environment

Biodegradation of Chemically Modified Gelatin Films in a Simulated Natural Environment

Protein-based films cross-linked with 1-ethyl-3-(3-dimethylamino-propyl) carbodiimide hydrochloride (EDC): effects of the cross-linker and film composition on the permeation rate of p-hydroxyacetanilide as a model drug

Rheological and biological characteristics of hyaluronic acid derivative modified by polyethylene glycol

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