Cross-linked xenogenic collagen implantation in the sheep model for vaginal surgery

Endo, Masayuki; Urbánková, Iva; Vlacil, J; Sengupta, Siddarth; Deprest, Thomas; Klosterhalfen, B.; Feola, Andrew; Deprest, Jan

doi:10.1007/s10397-015-0883-7

Cited by 17 publications

(20 citation statements)

References 45 publications

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“…Lastly, the ovine levator ani muscle retracts the anus yet not truly supporting pelvic organs, Gynecol Obstet Invest 2017;82:582-591 DOI: 10.1159/000454771 590 which rest rather on the bony pelvic floor. Our dissections confirmed the presence of extravaginal spaces of relevance to surgical experimentation [10][11][12]. Given the different urethral insertion point, dissection of the ventral vaginal wall is more difficult in its lower part, yet feasible above the urethra.…”

Section: Resultssupporting

confidence: 50%

“…Those ewes display collagen metabolism changes, yet the role of estrogens remains unclear [9]. Translational researchers increasingly use this model; however, there is a need for a detailed comparative anatomical study [10][11][12]. Although anatomical descriptions of relevant pelvic structures can be found in veterinarian text books, we found they had insufficient detail for the translational and clinical researchers.…”

Section: Introductionmentioning

confidence: 78%

“…Sheep are cheap, easily accessible, and there are less ethical constraints than with non-human primates. Consequently, we and others have started to use this model for vaginal surgery and biomechanics [10,11,24,25].…”

Section: Resultsmentioning

confidence: 99%

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Comparative Anatomy of the Ovine and Female Pelvis

Urbánková

Vdoviaková²,

Rynkevic³

et al. 2017

Gynecol Obstet Invest

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Background: Pelvic organ prolapse affects half of vaginally parous women. Several animal models are used to study its pathophysiology and treatment. Sheep are interesting because they develop spontaneously prolapse with similar risk factors as women and can be used for vaginal surgery. This study describes ovine pelvis anatomy and compares it to women's pelvis to provide anatomical tools for translational researchers. Methods: MRI, pelvic dissections, and histology were used for detailed macro- and microscopic analysis of relevant anatomical structures in 6 nulliparous ewes. Results: Although sheep are quadrupeds, the gross and microscopic anatomies are similar to the female pelvis. Principal differences are the shape and its orientation, the absence of the sacrospinous ligament and the internal obturator. The levator ani (except for the puborectalis) and the coccygeus muscle are present, yet the latter is more developed - coinciding with the tail. The dimensions and morphology of the ovine vagina is comparable. The retropubic and the rectovaginal space are accessible transvaginally. There is a wide expression of estrogen receptors with low or absent immunoreactivity in the urethral epithelium, bladder, anus and internal anal sphincter. Conclusion: The ovine pelvic floor has many anatomical and ultrastructural similarities to the female pelvic floor.

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Section: Resultssupporting

confidence: 50%

Section: Introductionmentioning

confidence: 78%

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Comparative Anatomy of the Ovine and Female Pelvis

Urbánková

Vdoviaková²,

Rynkevic³

et al. 2017

Gynecol Obstet Invest

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“…These studies reported discordant results [17,46]. Another study in which contractility was tested, did so for the purpose of documenting the impact of foreignmaterial implantation in pelvic floor reconstruction [47]. In our sheep, we saw an increase in contractility when the ewes became reproductive and a drop at menopause.…”

Section: Discussionmentioning

confidence: 62%

Morphological and Functional Changes in the Vagina following Critical Lifespan Events in the Ewe

Hympánová

Rynkevic

Urbánková

et al. 2019

Gynecol Obstet Invest

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Background/Aims: The ewe is increasingly being used as an animal model for pelvic floor disorders. The aim was to further characterize changes in the vaginal properties during its entire lifespan. Methods: Vaginal tissues were collected at different stages of reproductive life (neonatal, prepubescence, nulliparous, primiparous, multiparous, and menopausal; ≥6 ewes/group). Vaginal size, as well as active and passive biomechanics, was measured. Microscopy included thickness of glycogen, epithelium, lamina propria and muscularis thickness, densities of collagen, elastin, smooth muscle, and nerves. Results: Vaginal dimensions increase during adolescence, peak at reproductive levels, and decrease sharply after ovariectomy. One year after first delivery, the distal vagina gets more compliant, yet this is reversed later in life. The thickness of glycogen staining epithelial layers changed with puberty and menopause. The epithelium was markedly thicker after multiple deliveries. The thickness of lamina propria and muscularis increased in puberty and in nulliparous. Semi-quantitative collagen assessment demonstrated a lower collagen and higher elastin content after first and multiple deliveries. Conclusion: The changes in the ovine vaginal wall during representative moments of her lifespan parallel those observed in women.

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“…Biologically derived materials are classed as acellular collagen matrices and are often derived from the dermis, pericardium, small intestinal submucosa or urinary bladder matrix of bovine or porcine origin [1, 22, 59]. These materials can undergo crosslinking, the formation of excessive intra- and inter-molecular bonds, to improve the longevity of the material [59].…”

Section: Materials Discussionmentioning

confidence: 99%

In vivo response to polypropylene following implantation in animal models: a review of biocompatibility

Kelly¹,

Macdougall²,

Olabisi³

et al. 2016

Int Urogynecol J

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Introduction and hypothesisPolypropylene is a material that is commonly used to treat pelvic floor conditions such as pelvic organ prolapse (POP) and stress urinary incontinence (SUI). Owing to the nature of complications experienced by some patients implanted with either incontinence or prolapse meshes, the biocompatibility of polypropylene has recently been questioned. This literature review considers the in vivo response to polypropylene following implantation in animal models. The specific areas explored in this review are material selection, impact of anatomical location, and the structure, weight and size of polypropylene mesh types.MethodsAll relevant abstracts from original articles investigating the host response of mesh in vivo were reviewed. Papers were obtained and categorised into various mesh material types: polypropylene, polypropylene composites, and other synthetic and biologically derived mesh.ResultsPolypropylene mesh fared well in comparison with other material types in terms of host response. It was found that a lightweight, large-pore mesh is the most appropriate structure.ConclusionThe evidence reviewed shows that polypropylene evokes a less inflammatory or similar host response when compared with other materials used in mesh devices.Electronic supplementary materialThe online version of this article (doi:10.1007/s00192-016-3029-1) contains supplementary material, which is available to authorized users

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Cross-linked xenogenic collagen implantation in the sheep model for vaginal surgery

Cited by 17 publications

References 45 publications

Comparative Anatomy of the Ovine and Female Pelvis

Comparative Anatomy of the Ovine and Female Pelvis

Morphological and Functional Changes in the Vagina following Critical Lifespan Events in the Ewe

In vivo response to polypropylene following implantation in animal models: a review of biocompatibility

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