Cross-linked enzyme crystals of organophosphate hydrolase for electrochemical detection of organophosphorus compounds

Laothanachareon, Thanaporn; Champreda, Verawat; Sritongkham, Pornpimol; Somasundrum, Mithran; Surareungchai, Werasak

doi:10.1007/s11274-008-9851-y

Cited by 38 publications

(23 citation statements)

References 34 publications

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“…The optimum pH for present method was 8.0 ( Figure 5 ) which is higher than pH-7.4 [ 19 , 23 , 30 , 31 ], pH 7.0 [ 32 , 33 ], and lower than pH 8.8 [ 34 ] and pH 9.5 [ 17 ]. This pH is similar to those reported in previous study [ 35 ].…”

Section: Resultssupporting

confidence: 92%

Preparation of Electrochemical Biosensor for Detection of Organophosphorus Pesticides

Gothwal

Beniwal

Dhull

et al. 2014

International Journal of Analytical Chemistry

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Polyvinyl chloride (PVC) can be used to develop reaction beaker which acts as electrochemical cell for the measurement of OP pesticides. Being chemically inert, corrosion resistant, and easy in molding to various shapes and size, PVC can be used for the immobilization of enzyme. Organophosphorus hydrolase was immobilized covalently onto the chemically activated inner surface of PVC beaker by using glutaraldehyde as a coupling agent. The carbon nanotubes paste working electrode was constructed for amperometric measurement at a potential of +0.8 V. The biosensor showed optimum response at pH 8.0 with incubation temperature of 40°C. K m and I max for substrate (methyl parathion) were 322.58 µM and 1.1 µA, respectively. Evaluation study showed a correlation of 0.985, which was in agreement with the standard method. The OPH biosensor lost 50% of its initial activity after its regular use for 25 times over a period of 50 days when stored in 0.1 M sodium phosphate buffer, pH 8.0 at 4°C. No interference was observed by interfering species.

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Section: Resultssupporting

confidence: 92%

Preparation of Electrochemical Biosensor for Detection of Organophosphorus Pesticides

Gothwal

Beniwal

Dhull

et al. 2014

International Journal of Analytical Chemistry

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“…For both free and immobilized enzymes compare to previous works where GOx were entrapped on the electrode surface via different CPs, lower K m value was observed. This may as well be due to the CLEC‐like structure on the PTBT matrix that exhibits higher affinity toward the glucose substrate as the other CLEC or CLEA structures 35…”

Section: Resultsmentioning

confidence: 99%

Electrochemical Polymerization of (2‐Dodecyl‐4, 7‐di (thiophen‐2‐yl)‐2H‐benzo[d][1,2,3] triazole): A Novel Matrix for Biomolecule Immobilization

Ekiz

Yüksel

Balan

et al. 2010

Macromolecular Bioscience

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A recently synthesized conducting polymer [poly(2-dodecyl-4,7-di(thiophen-2-yl)-2H-benzo[d][1,2,3]triazole (PTBT)] was tested as a platform for biomolecule immobilization. After electrochemical polymerization of the monomer (TBT) on graphite electrodes, immobilization of glucose oxidase (GOx,β-D-glucose: oxygen-1-oxidoreductase, EC 1.1.3.4) was carried out. To improve the interactions between the enzyme and hydrophobic alkyl chain on the polymeric structure, GOx and isoleucine (Ile) amino acid were mixed in sodium phosphate buffer (pH 7.0) with a high ionic strength (250 × 10(-3) M). The solution is then casted on the polymer film, and the amino groups in the protein structure were crosslinked using glutaraldehyde (GA) as the bifunctional agent. Finally, the surface was covered with a perm-selective membrane. Consequently, cross-linked enzyme crystal (CLEC) like assembles with regular shapes were observed after immobilization. Microscopic techniques such as scanning electron microscopy (SEM) and fluorescence microscopy were used to monitor the surface morphologies of both the polymer and the bioactive layer. Electrochemical responses of the enzyme electrodes were measured by monitoring O(2) consumption in the presence of glucose at -0.7 V. The optimized biosensor showed a very good linearity between 0.05 and 2.5 × 10(-3) M with a 52 s response time and a detection limit (LOD) of 0.029 × 10(-3) M to glucose. Also, kinetic parameters, operational and storage stabilities were determined. K(m) and I(max) values were found as 4.6 × 10(-3) M and 2.49 µA, respectively. It was also shown that no activity was lost during operational and storage conditions. Finally, proposed system was applied for glucose biomonitoring during fermentation in yeast culture where HPLC was used as the reference method to verify the data obtained by the proposed biosensor.

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“…entrapment) [43]. Microorgani ms have several advantages s [194] Paraoxon Amperometry Cross-linking MWNT 5 × 10 -7 -2 × 10 -6 0.314 × 10 -6 [195] Paraoxon Optic Cross-linking -10 -5 -4.8 × 10 -4 2 × 10 -6 [196] Ethyl Parathion Amperometry Covalent binding -ND <3.4 × 10 -9 [197] Methyl parathion Amperometry Covalent binding AuNPs -MWCN-QDs 1.9 × 10 -8 -7.6 × 10 -7 3.8 × 10 -9 [39] Methyl parathion Amperometry Entrapment MCNT Up to 2 × 10 -6 8 × 10 -7 [194] Parathion Optic Cross-linking -10 -5 -2 × 10 -4 2 × 10 -6 [196] Paraoxon Fluorescence Affinity -10 -6 -8 × 10 -4 ND [198] Diisopropyl phosphorofluoridate (DFP) Fluorescence Affinity -2 × 10 -6 -4 × 10 -4 ND [198] as sensing elements in the development of biosensors. They are able to metabolise a wide range of chemical compounds.…”

Section: Microbial Biosensorsmentioning

confidence: 99%

Biosensors for Pesticide Detection: New Trends

Sassolas¹,

Prieto‐Simón²,

Marty³

2012

AJAC

185

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Due to the large amounts of pesticides commonly used and their impact on health, prompt and accurate pesticide analysis is important. This review gives an overview of recent advances and new trends in biosensors for pesticide detection. Optical, electrochemical and piezoelectric biosensors have been reported based on the detection method. In this review biosensors have been classified according to the immobilized biorecognition element: enzymes, cells, antibodies and, more rarely, DNA. The use of tailor-designed biomolecules, such as aptamers and molecularly imprinted polymers, is reviewed. Artificial Neural Networks, that allow the analysis of pesticide mixtures are also presented. Recent advances in the field of nanomaterials merit special mention. The incorporation of nanomaterials provides highly sensitive sensing devices allowing the efficient detection of pesticides

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Cross-linked enzyme crystals of organophosphate hydrolase for electrochemical detection of organophosphorus compounds

Cited by 38 publications

References 34 publications

Preparation of Electrochemical Biosensor for Detection of Organophosphorus Pesticides

Preparation of Electrochemical Biosensor for Detection of Organophosphorus Pesticides

Electrochemical Polymerization of (2‐Dodecyl‐4, 7‐di (thiophen‐2‐yl)‐2H‐benzo[d][1,2,3] triazole): A Novel Matrix for Biomolecule Immobilization

Biosensors for Pesticide Detection: New Trends

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