Cross-Linked Collagen-Based Scaffold: Anti-infective Potential, Hydrophilic, and Biocompatible

Abstract

Porcine decellularized dermal matrix (pADM) was a type of collagen-based medical scaffold material with excellent biological properties. Because of the lack of antibacterial properties, the wound infection caused by bacteria during the treatment process greatly affected the recovery and even the life safety of patients. In this paper, a creative design idea of antimicrobial material was proposed based on free radical polymerization reaction. The amphoteric monomer sulfobetaine methacrylate (SBMA) was employed to cross-link with methacrylated pADM (MA-pADM) by free radical polymerization to obtain cross-linked pADM grafted with amphoteric ions (C-MA-SBMA-pADM). The collagen scaffold after cross-linking had sufficient collagen stability, proper mechanical properties, and retained the natural structure of collagen while introducing hydrophilic sulfonic acid groups and quaternary ammonium groups, and C-MA-SBMA-pADM exhibited excellent hydrophilic properties by water contact angle detection. The results of cellular experiments showed that the scaffold had good cytocompatibility. Escherichia coli and Staphylococcus aureus were used to test the antibacterial properties of the material, and the results showed that the cross-linked C-MA-SBMA-pADM had good antibacterial effects. In conclusion, a cross-linked collagen-based scaffold was prepared with good mechanical properties, cytocompatibility, and also excellent antibacterial properties, which was expected to be more widely used in the field of tissue engineering scaffold materials.

Balanced chemical reactivity, antimicrobial properties and biocompatibility of decellularized dermal matrices for wound healing

“…8,9 The pADM material can close the wound surface, avoid wound exposure and maintain local wetness of the wound, and also provide support for epidermal cell growth, which promotes epidermal growth and wound healing, and therefore it is widely used in clinics for skin restoration. [10][11][12] However, the main component of the pADM is collagen fibers, 13 which do not have antimicrobial properties by themselves and have the disadvantage of being susceptible to bacterial infection in the treatment of incompletely debrided wounds, 14,15 so they need to be given the ability to resist bacterial infection in order to improve the quality of wound healing and reduce patient pain. 16,17 Chitosan quaternary ammonium salt is the product of grafting a quaternary ammonium group from chitosan, and not only solves the solubility problem of chitosan but also maintains the natural structure of chitosan with good biocompatibility.…”