Cross‐Link‐Functionalized Nanoparticles for Rapid Excretion in Nanotheranostic Applications

Ma, Zhuoran; Wang, Feifei; Zhong, Yongwang; Salazar, Felix B.; Li, Jiachen; Zhang, Mingxi; Ren, Fuqiang; Wu, Anna M.; Dai, Hongjie

doi:10.1002/ange.202008083

Cited by 8 publications

(6 citation statements)

References 73 publications

(76 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There is significant room for deploying NIR-II multiplexed molecular imaging for biology and medicine (17,(27)(28)(29)(30)(31)(32)(33)(34)(35)(36)(37)(38)(39)(40)(41)(42). This work demonstrated three-plex in vivo molecular imaging by NIR-II SIM to investigate immune cells in response to immunotherapy with high spatial resolution.…”

Section: Discussionmentioning

confidence: 97%

“…Twenty-four hours later, erbium-based rare-earth down-conversion nanoparticles (ErNPs) (30, 31) (excitation, 975 nm; emission, 1,500 to 1,700 nm) conjugated to anti-CD8 cys-diabody (61) (aCD8-ErNPs) and PEGylated PbS/CdS CSQD (excitation, 1,540 nm; emission, 1,600 to 1,700 nm) conjugated to anti-OX40 (aOX40-PbS) were injected intravenously through the tail vein. The blood circulation half times of PbS and ErNPs were ∼4.8 and ∼5.2 h, respectively (31,42), over which these nanoprobes extravasated in the tumor as clearly imaged by in vivo LSM imaging in a noninvasive manner (SI Appendix, Fig. S6).…”

Section: Noninvasive In Vivo Nir-ii Sim Of Tumor Microenvironment Post-mentioning

confidence: 99%

“…Recently we developed near-infrared II (NIR-II) LSM employing one-photon excitation and fluorescence emission detection in the near-infrared II window (1,000 to 1,700 nm) using fluorescent or luminescent organic dyes, quantum dots, and rare-earth downconversion nanoparticles (17,(27)(28)(29)(30)(31)(32)(33)(34)(35)(36)(37)(38)(39)(40)(41)(42). The suppressed light scattering and reduced tissue autofluorescence allowed noninvasive in vivo NIR-II LSM imaging through the scalp and skull of intact mouse head without craniotomy and through skin imaging of tumors without installing invasive optical windows, with a penetration depth up to ∼750 μm (17).…”

mentioning

confidence: 99%

See 2 more Smart Citations

In vivo NIR-II structured-illumination light-sheet microscopy

Wang

Zhong

et al. 2021

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

View full text Add to dashboard Cite

Noninvasive optical imaging with deep tissue penetration depth and high spatiotemporal resolution is important to longitudinally studying the biology at the single-cell level in live mammals, but has been challenging due to light scattering. Here, we developed near-infrared II (NIR-II) (1,000 to 1,700 nm) structured-illumination light-sheet microscopy (NIR-II SIM) with ultralong excitation and emission wavelengths up to ∼1,540 and ∼1,700 nm, respectively, suppressing light scattering to afford large volumetric three-dimensional (3D) imaging of tissues with deep-axial penetration depths. Integrating structured illumination into NIR-II light-sheet microscopy further diminished background and improved spatial resolution by approximately twofold. In vivo oblique NIR-II SIM was performed noninvasively for 3D volumetric multiplexed molecular imaging of the CT26 tumor microenvironment in mice, longitudinally mapping out CD4, CD8, and OX40 at the single-cell level in response to immunotherapy by cytosine-phosphate-guanine (CpG), a Toll-like receptor 9 (TLR-9) agonist combined with OX40 antibody treatment. NIR-II SIM affords an additional tool for noninvasive volumetric molecular imaging of immune cells in live mammals.

show abstract

Section: Discussionmentioning

confidence: 97%

Section: Noninvasive In Vivo Nir-ii Sim Of Tumor Microenvironment Post-mentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

In vivo NIR-II structured-illumination light-sheet microscopy

Wang

Zhong

et al. 2021

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Moreover, the long-term stabilities of PAA-coated Nd@Y NPs (Nd@Y-PAA) and Er@Y NPs (Er@Y-PAA) were verified by monitoring the NIR-II brightness after storing them in PBS and FBS for over 5 days, and the plotted result indicates that these NPs are very stable for long-term storage and in vivo illumination (Figure 1I). With the water-soluble lanthanide-doped nanoparticles in hand, we further grafted a polymeric layer of mixed methoxy polyethylene glycol amine (mPEG-NH 2 ) and 8 Arm-PEG-NH 2 (ratio = 1:5, see methods in Supplementary Material) on both two PAA-coated nanoparticles, aiming to form high cross-linking hydrophilic polymeric layers and simultaneously impart amine groups for the following targeting-moiety conjugation (Figure S6) [55][56][57]. To achieve the targeted components of NIR-II ratiometric probes, we conjugated FA (a small molecule for targeting folate receptors) to PEGylated Nd 3+ -doped nanoparticles through 1-(3-dimethyl-aminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) chemistry (see methods in Supplementary Material).…”

Section: Design and Synthesis Of Nir-ii Ratiometric Fluorescence Probesmentioning

confidence: 99%

Near-infrared-II ratiometric fluorescence probes for non-invasive detection and precise navigation surgery of metastatic sentinel lymph nodes

Li¹,

Xue²,

Han³

et al. 2022

Theranostics

View full text Add to dashboard Cite

Sentinel lymph node (SLN) biopsy is the key diagnostic procedure to determine tumor metastasis and treatment plan. Current SLN biopsy has considerable drawbacks in that SLNs (both malignant and normal) must be removed by navigation surgery, followed by a time-consuming pathological examination. The selective, non-invasive, and real-time diagnosis of metastatic status in SLNs is becoming essential. Methods: Here, we design two lanthanide-doped nanoparticles as a pair of NIR-II ratiometric fluorescence probes, one of which is conjugated with tumor-targeting moiety, while the other is conjugated with PEG as an internal reference. The NIR-II ratiometric fluorescence signal (I 1060 nm /I 1525 nm ) from two well-separated channels were used to identify the tumor-draining SLNs. The precise navigation surgery of metastatic SLNs was performed and we further evaluated their surgery outcomes. Results: The NIR-II ratiometric fluorescence facilitates an ideal fluorescence-guided surgery with only resection of tumor-positive SLNs, thereby avoiding unnecessary removal of the normal SLNs. In addition, our system has a time-saving operation procedure and can be performed under the operation light without altering the appearance of surgical settings. Conclusion: The present study enables non-invasive and real-time detection metastatic status in SLNs with high sensitivity and selectivity. Our investigations will provide a new direction for SLN biopsy and substantially improve cancer surgery outcomes.

show abstract

“…2b), larger than our previous ~ 6.9 nm QD with emission peak in NIR-IIb (named 'QDb') 12 . To transfer the QDc from organic phase to aqueous phase, a hydrophilic polymeric cross-linked network (P 3 coating) developed by our group was coated on the QDc to impart biocompatibility in physiological environments and biliary excretion in ~ 2 weeks without apparent toxic effects 14,26 . The final aqueous stabilized P 3 -QDc exhibited a peak emission wavelength of ~ 1820 nm in NIR-IIc (Fig.…”

mentioning

confidence: 99%

Non-Invasive Confocal Fluorescence Imaging of Mice Beyond 1700 nm Using Superconducting Nanowire Single-Photon Detectors

Wang

Ren

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

Light scattering by biological tissues sets a limit to the penetration depth of high-resolution optical microscopy imaging of live mammals in vivo. An effective approach to reduce light scattering and increase imaging depth is by extending the excitation and emission wavelengths to the > 1000 nm second near-infrared (NIR-II), also called the short-wavelength infrared (SWIR) window. Here, we developed biocompatible core-shell lead sulfide/cadmium sulfide (PbS/CdS) quantum dots emitting at ~1880 nm and superconducting nanowire single photon detectors (SNSPD) for single-photon detection up to 2000 nm, enabling one-photon fluorescence imaging window in the 1700-2000 nm (NIR-IIc) range. Confocal fluorescence imaging in NIR-IIc reached an imaging depth of ~ 800 μm through intact mouse head, and enabled non-invasive imaging of inguinal lymph nodes (LNs) without any surgery. In vivo molecular imaging of high endothelial venules (HEVs) with diameter down to ~ 6.6 μm in the lymph nodes was achieved, opening the possibility of non-invasive imaging of immune trafficking in lymph nodes at the single-cell/vessel level longitudinally.

show abstract

Cross‐Link‐Functionalized Nanoparticles for Rapid Excretion in Nanotheranostic Applications

Cited by 8 publications

References 73 publications

In vivo NIR-II structured-illumination light-sheet microscopy

In vivo NIR-II structured-illumination light-sheet microscopy

Near-infrared-II ratiometric fluorescence probes for non-invasive detection and precise navigation surgery of metastatic sentinel lymph nodes

Non-Invasive Confocal Fluorescence Imaging of Mice Beyond 1700 nm Using Superconducting Nanowire Single-Photon Detectors

Contact Info

Product

Resources

About