Cross-kingdom microbial interactions in dental implant-related infections: is Candida albicans a new villain?

Souza, João Gabriel Silva; Costa, Raphael Cavalcante; Sampaio, Aline Araújo; Abdo, Victoria L.; Nagay, B; Castro, Nidia; Retamal‐Valdes, Belén; Shibli, Jamil Awad; Feres, Magda; Barão, Valentim Adelino Ricardo; Bertolini, Martinna

doi:10.1016/j.isci.2022.103994

Cited by 24 publications

(11 citation statements)

References 159 publications

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“…Previous studies have shown that Ca and Porphyromonas gingivalis form a community through InlJ-Als3-dependent binding and reported that P. gingivalis within the community may increase pathogenicity [ 37 ]. João et al [ 38 ] reported that the interaction between Ca and bacteria involves the environment, the composition of the microbiota, and the host immune response and pointed out that most studies have focused on the interaction between Ca and individual oral bacterial species. However, few studies have investigated the interactions between Ca and polymicrobial biofilms.…”

Section: Discussionmentioning

confidence: 99%

Effects of Candidalysin Derived from Candida albicans on the Expression of Pro-Inflammatory Mediators in Human Gingival Fibroblasts

Nishikawa

Tomotake

Kawano

et al. 2023

IJMS

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Candida albicans (Ca) is frequently detected in the peri-implant sulcus with peri-implantitis, a major postoperative complication after oral implant therapy. However, the involvement of Ca in the pathogenesis of peri-implantitis remains unclear. In this study, we aimed to clarify Ca prevalence in the peri-implant sulcus and investigated the effects of candidalysin (Clys), a toxin produced by Ca, on human gingival fibroblasts (HGFs). Peri-implant crevicular fluid (PICF) was cultured using CHROMagar and Ca colonization rate and colony numbers were calculated. The levels of interleukin (IL)-1β and soluble IL-6 receptor (sIL-6R) in PICF were quantified by enzyme-linked immunosorbent assay (ELISA). Pro-inflammatory mediator production and intracellular signaling pathway (MAPK) activation in HGFs were measured by ELISA and Western blotting, respectively. The Ca colonization rate and the average number of colonies in the peri-implantitis group tended to be higher than those in the healthy group. IL-1β and sIL-6R levels in the PICF were significantly higher in the peri-implantitis group than in the healthy group. Clys significantly induced IL-6 and pro-matrix metalloproteinase (MMP)-1 productions in HGFs, and co-stimulation with Clys and sIL-6R increased IL-6, pro-MMP-1, and IL-8 production levels in HGFs compared with Clys stimulation alone. These findings suggest that Clys from Ca plays a role in the pathogenesis of peri-implantitis by inducing pro-inflammatory mediators.

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Section: Discussionmentioning

confidence: 99%

Effects of Candidalysin Derived from Candida albicans on the Expression of Pro-Inflammatory Mediators in Human Gingival Fibroblasts

Nishikawa

Tomotake

Kawano

et al. 2023

IJMS

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“…aureus can be associated with human diseases, as dental implant-related infections, and C. albicans is a yeast related to infections, including periodontal and dental implant-related infections . For this, a pool of fresh stimulated human saliva was processed according to the abovementioned methods (0.22 μm filter, 0.45 μm filter, and pasteurization).…”

Section: Methodsmentioning

confidence: 99%

“…S. mutans is a bacterium that metabolizes sucrose to produce an extracellular polysaccharide matrix that stimulates microbial colonization, 22 A. naeslundii is a well-known first colonizer of oral surfaces, 20 S. aureus can be associated with human diseases, as dental implant-related infections, 42 and C. albicans is a yeast related to infections, including periodontal 43 and dental implant-related infections. 44 For this, a pool of fresh stimulated human saliva was processed according to the abovementioned methods (0.22 μm filter, 0.45 μm filter, and pasteurization). Then, processed saliva was used for salivary pellicle adsorption on a plastic (polystyrene) surface in a 24well plate.…”

Section: Microbial Adhesionmentioning

confidence: 99%

Effect of Processing Methods of Human Saliva on the Proteomic Profile and Protein-Mediated Biological Processes

et al. 2023

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The use of saliva as a protein source prior to microbiological and biological assays requires previous processing. However, the effect of these processing methods on the proteomic profile of saliva has not been tested. Stimulated human saliva was collected from eight healthy volunteers. Non-processed saliva was compared with 0.22 μm filtered, 0.45 μm filtered, and pasteurized saliva, by liquid chromatography−mass spectrometry. Data are available via ProteomeXchange with identifier PXD039248. The effect of processed saliva on microbial adhesion was tested using bacterial and fungus species and in biological cell behavior using HaCaT immortalized human keratinocytes. Two hundred and seventy-eight proteins were identified in non-processed saliva, of which 54 proteins (≈19%) were exclusive. Saliva processing reduced identified proteins to 222 (≈80%) for the 0.22 μm group, 219 (≈79%) for the 0.45 μm group, and 201 (≈72%) for the pasteurized saliva, compared to non-processed saliva. The proteomic profile showed similar molecular functions and biological processes. The different saliva processing methods did not alter microbial adhesion (ANOVA, p > 0.05). Interestingly, pasteurized saliva reduced keratinocyte cell viability. Saliva processing methods tested reduced the proteomic profile diversity of saliva but maintained similar molecular functions and biological processes, not interfering with microbial adhesion and cell viability, except for pasteurization, which reduced cell viability.

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“…are proficient colonizers of micro-rough Ti surfaces, there are serious implications with regard to infection. [35][36] Regardless, the antifungal mechanism of nano-and microstructured topographies, including tubes, wires, pillars, etc., has not been illuminated. C. albicans cells were not effectively killed by a microcone array on silicon (Si) substrata as they were observed to rest inbetween the microcones on silicon surfaces (height: 3.5 μm, base width: 630 nm) due to the vast distance (3.7 μm) between the microcones.…”

Section: Introductionmentioning

confidence: 99%

Apoptosis of Multi‐Drug Resistant Candida Species on Microstructured Titanium Surfaces

Le,

Linklater,

Aburto‐Medina

et al. 2023

Adv Materials Inter

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The proportion of hospital‐acquired medical device infections caused by pathogenic, multi‐drug resistant Candida species occurs in up to 10% of implantations. In this study, a unique antifungal micro‐pillared titanium surface pattern is developed, which demonstrates both fungicidal and fungistatic activity, persistently deterring biofilm formation by Candida albicans and multi‐drug resistant Candida auris fungi for up to 7 days. The Ti micropillars of 3.5 µm height are fabricated using maskless inductively coupled plasma reactive ion etching. The micro‐textured surface consistently kills ≈50% of Candida spp. irreversibly attached cells and prevent the proliferation of the remaining cells by inducing programmed cell death. Proteomic analysis reveals that Candida cells undergo extensive metabolic stress, preventing the transformation from yeast to the filamentous/hyphal cell phenotype that is essential for establishing a typical in vitro biofilm. The mechanical stress imparted following interaction with the micropillars injures attaching cells and induces apoptosis whereby the Candida cells are unable to be revived in a non‐stress environment. These findings shed new insight toward the design of durable antifungal surfaces that prevent biofilm formation by pathogenic, multi‐drug resistant yeasts.

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Cross-kingdom microbial interactions in dental implant-related infections: is Candida albicans a new villain?

Cited by 24 publications

References 159 publications

Effects of Candidalysin Derived from Candida albicans on the Expression of Pro-Inflammatory Mediators in Human Gingival Fibroblasts

Effects of Candidalysin Derived from Candida albicans on the Expression of Pro-Inflammatory Mediators in Human Gingival Fibroblasts

Effect of Processing Methods of Human Saliva on the Proteomic Profile and Protein-Mediated Biological Processes

Apoptosis of Multi‐Drug Resistant Candida Species on Microstructured Titanium Surfaces

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