Cross-Inoculation of Elite Commercial Bradyrhizobium Strains from Cowpea and Soybean in Mung Bean and Comparison with Mung Bean Isolates

Favero, Vinício Oliosi; Carvalho, Rita Hilário de; Leite, Ana Beatriz Carneiro; Santos, Dieini Melissa Teles dos; Freitas, Karine Moura de; Zilli, Jerri Édson; Xavier, Gustavo Ribeiro; Rumjanek, Norma Gouvêa; Caballero, Segundo Sacramento Urquiaga

doi:10.1007/s42729-022-01034-0

Cited by 2 publications

(2 citation statements)

References 34 publications

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“…The cross-section of the root nodules collected from the infected G. max indicated the presence of leghemoglobin (hemeprotein). Leghemoglobin is a symbiotic hemoglobin protein that creates the anaerobic conditions inside the nodules which is necessary for the effective symbiotic nitrogen fixation [ 11 , 12 , 45 ].…”

Section: Discussionmentioning

confidence: 99%

Nodulation Experiment by Cross-Inoculation of Nitrogen-Fixing Bacteria Isolated from Root Nodules of Several Leguminous Plants

Cho,

Joshi,

Hur

et al. 2023

J. Microbiol. Biotechnol.

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Root-nodule nitrogen-fixing bacteria are known for being specific to particular legumes. This study isolated the endophytic root-nodule bacteria from the nodules of legumes and examined them to determine whether they could be used to promote the formation of nodules in other legumes. Forty-six isolates were collected from five leguminous plants and screened for housekeeping (16S rRNA), nitrogen fixation ( nifH ), and nodulation ( nodC ) genes. Based on the 16S rRNA gene sequencing and phylogenetic analysis, the bacterial isolates WC15, WC16, WC24, and GM5 were identified as Rhizobium , Sphingomonas , Methylobacterium , and Bradyrhizobium , respectively. The four isolates were found to have the nifH gene, and the study confirmed that one isolate (GM5) had both the nifH and nodC genes. The Salkowski method was used to measure the isolated bacteria for their capacity to produce phytohormone indole acetic acid (IAA). Additional experiments were performed to examine the effect of the isolated bacteria on root morphology and nodulation. Among the four tested isolates, both WC24 and GM5 induced nodulation in Glycine max . The gene expression studies revealed that GM5 had a higher expression of the nifH gene. The existence and expression of the nitrogen-fixing genes implied that the tested strain had the ability to fix the atmospheric nitrogen. These findings demonstrated that a nitrogen-fixing bacterium, Methylobacterium (WC24), isolated from a Trifolium repens , induced the formation of root nodules in non-host leguminous plants ( Glycine max ). This suggested the potential application of these rhizobia as biofertilizer. Further studies are required to verify the N 2 -fixing efficiency of the isolates.

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Section: Discussionmentioning

confidence: 99%

Nodulation Experiment by Cross-Inoculation of Nitrogen-Fixing Bacteria Isolated from Root Nodules of Several Leguminous Plants

Cho,

Joshi,

Hur

et al. 2023

J. Microbiol. Biotechnol.

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“…Cross-nodulation with different Bradyrhizobium strains has also been observed. Three out of four Bradyrhizobium strains used in Brazil as a commercial inoculant for cowpea, B. viridifuturi , B. yuanmingense , and B. elkanii, successfully nodulate mung bean, but not B. pachyrhizi [ 93 ]. These results indicate the existence of some level of incompatibility between strains of the Bradyrhizobium genus in relation to the colonization of mung bean nodule colonization.…”

Section: Microsymbionts Inhabitants Of Pulse Nodulesmentioning

confidence: 99%

Contribution of Biofertilizers to Pulse Crops: From Single-Strain Inoculants to New Technologies Based on Microbiomes Strategies

Xavier

Jesus

Dias

et al. 2023

Plants

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Pulses provide distinct health benefits due to their low fat content and high protein and fiber contents. Their grain production reaches approximately 93,210 × 103 tons per year. Pulses benefit from the symbiosis with atmospheric N2-fixing bacteria, which increases productivity and reduces the need for N fertilizers, thus contributing to mitigation of environmental impact mitigation. Additionally, the root region harbors a rich microbial community with multiple traits related to plant growth promotion, such as nutrient increase and tolerance enhancement to abiotic or biotic stresses. We reviewed the eight most common pulses accounting for almost 90% of world production: common beans, chickpeas, peas, cowpeas, mung beans, lentils, broad beans, and pigeon peas. We focused on updated information considering both single-rhizobial inoculation and co-inoculation with plant growth-promoting rhizobacteria. We found approximately 80 microbial taxa with PGPR traits, mainly Bacillus sp., B. subtilis, Pseudomonas sp., P. fluorescens, and arbuscular mycorrhizal fungi, and that contributed to improve plant growth and yield under different conditions. In addition, new data on root, nodule, rhizosphere, and seed microbiomes point to strategies that can be used to design new generations of biofertilizers, highlighting the importance of microorganisms for productive pulse systems.

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Cross-Inoculation of Elite Commercial Bradyrhizobium Strains from Cowpea and Soybean in Mung Bean and Comparison with Mung Bean Isolates

Cited by 2 publications

References 34 publications

Nodulation Experiment by Cross-Inoculation of Nitrogen-Fixing Bacteria Isolated from Root Nodules of Several Leguminous Plants

Nodulation Experiment by Cross-Inoculation of Nitrogen-Fixing Bacteria Isolated from Root Nodules of Several Leguminous Plants

Contribution of Biofertilizers to Pulse Crops: From Single-Strain Inoculants to New Technologies Based on Microbiomes Strategies

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