Cross-grating phase microscopy (CGM): In silico experiment (insilex) algorithm, noise and accuracy

Wavefront Microscopy Using Quadriwave Lateral Shearing Interferometry: From Bioimaging to Nanophotonics

“…The further the adjustment, the better the sensitivity is. However, d has to remain within a reasonable range to avoid artifacts and loss of resolution …”

“…), which implies a camera pixel size of p = 7.4 μm and a grating pitch of Γ = 59.2 μm (i.e., a zeta-factor ζ = Γ/2 p = 4). More recent CGM cameras with ζ = 3 and Γ = 39 μm yield higher spatial resolution (Figure k) . In theory, because CGM and DHM image processing methods are both based on a filtering of spatial frequencies in the Fourier space, they both reduce the image definition in the exact same way.…”

“…By acquiring a series of 4 images corresponding to 4 different phase shifts (0, π/2, π, 3π/2), a quantitative phase image of the sample can be obtained. SLIM was shown to feature a noise amplitude of 0.3 nm on the OPD, similar to CGM The use of an SLM in SLIM makes the system not only significantly more complex but also polarization dependent.…”

“…However, d has to remain within a reasonable range to avoid artifacts and loss of resolution. 29 In practice, upon wavefront distortion, the bright-spot pattern gets distorted and displaced, and the displacements of the dots (barely visible to the naked eye) follow a simple shadow description as in ray-optics (Figure 3d). Note that this is exactly how SH wavefront sensing works.…”

“…However, after treatment of the DHM images, both techniques yielded 7k). 29 In theory, because CGM and DHM image processing methods are both based on a filtering of spatial frequencies in the Fourier space, they both reduce the image definition in the exact same way. It does not mean that the spatial resolution gets affected.…”