Cross-flow microfiltration of fermentation broth containing native corn starch

Białas, W.; Celińska, Ewelina; Dembczyński, Radosław; Szymanowska, Daria; Nowacka, Magdalena; Jesionowski, Teofil; Grajek, W.

doi:10.1016/j.memsci.2012.09.046

Cited by 14 publications

(6 citation statements)

References 41 publications

(66 reference statements)

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“…In contrast to the effect of TMP variation on the flux, the increase of CFV from 1.2 to 2.2 m·s −1 drove the flux up considerably from 29.0 to 53.0 L·m −2 ·h −1 . This effect corresponded to all the previous research efforts (19,45,77).…”

Section: Influence Of the Cross Flow Velocity On Filtrationsupporting

confidence: 85%

Lactic acid production in a membrane bioreactor system with thermophilicBacillus coagulans: fouling analysis of the used ceramic membranes

Fan

Ebrahimi

Quitmann

et al. 2015

Separation Science and Technology

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In lactic acid fermentation broth systems, membrane fouling is characterized as the crucial factor in limiting ceramic membrane lifetime and efficiency. This study aimed to find the limiting factor of this process and seek an effective solution. By quantitative analysis using resistance-in-series model, the cake layer resistance is demonstrated to be the dominant factor in a steady state. A cake compressibility index was measured as high as 2.09. Within the low transmembrane pressure range, the cake resistance was manipulated through crossflow velocity regulation to achieve a better membrane performance. A new correlation between flux and cell density was also introduced.

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Section: Influence Of the Cross Flow Velocity On Filtrationsupporting

confidence: 85%

Lactic acid production in a membrane bioreactor system with thermophilicBacillus coagulans: fouling analysis of the used ceramic membranes

Fan

Ebrahimi

Quitmann

et al. 2015

Separation Science and Technology

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“…Prior to SEM analysis, the samples of digested and nondigested starch granules were subjected to repeated washing with 20 mM NaOH and ddH 2 O, as previously described (Białas et al 2013), and subsequently lyophilized. Lyophilization was carried out in vials in Christ Beta lyophilizator (Christ, Germany), applying the following parameters: initial freezing −35 °C; drying: condenser −35 °C, shelf 15 °C, pressure 0.22 mbar, time 40 h; and final drying: shelf 20 °C, pressure 0.18 mbar, time 8 h. Samples were stored in a desiccator until analysis.…”

Section: Methodsmentioning

confidence: 99%

Cloning, expression, and purification of insect (Sitophilus oryzae) alpha-amylase, able to digest granular starch, in Yarrowia lipolytica host

Celińska

Białas

Borkowska

et al. 2014

Appl Microbiol Biotechnol

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Raw-starch-digesting enzymes (RSDE) are of major importance for industrial applications, as their usage greatly simplifies the starch processing pipeline. To date, only microbial RSDE have gained considerable attention, since only microbial production of enzymes meets industrial demands. In this study, α-amylase from rice weevil (Sitophilus oryzae), the major rice pest, was cloned and expressed in Yarrowia lipolytica Po1g strain. The enzyme was secreted into the culture medium, and the peak activity (81 AU/L) was reached after only 29 h of culturing in 5-L bioreactors. Through simple purification procedure of ammonium sulfate precipitation and affinity chromatography, it was possible to purify the enzyme to apparent homogeneity (25-fold purification factor, at 5 % yield). The optimal conditions for the α-amylase activity were pH 5.0 and a temperature of 40 °C. The α-amylase studied here did not show any obligate requirement for Ca2+ ions. The recombinant α-amylase appeared to efficiently digest granular starch from pea, amaranth, waxy corn, and waxy rice.

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“…Resistance analysis is a very effective method in order to the determine the phenomena leading to permeate flux decline during filtration processes [25]. Thus, in several previous studies [2,8,11,13,14,16,19,24,25,37,[42][43][44][45] the resistance-in series model has been successfully applied to analyze the reduction of permeate flux during cross-flow MF of various microbial media. This model classified fouling resistance into resistances of: membrane, polarization, adsorption, as well as cake built on the membrane surface.…”

Section: Introductionmentioning

confidence: 99%

Cross-Flow Microfiltration of Glycerol Fermentation Broths with Citrobacter freundii

Tomczak

Gryta

2020

Membranes

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This paper reports the study of the cross-flow microfiltration (MF) of glycerol fermentation broths with Citrobacter freundii bacteria. A single channel tubular ceramic membrane with a nominal pore size of 0.14 µm was used. It has been demonstrated that the MF ceramic membrane has been successfully applied to bacteria cell removal and to effectively eliminate colloidal particles from glycerol fermentation broths. However, due to fouling, the significant reduction of the MF performance has been demonstrated. In order to investigate the impact of transmembrane pressure (TMP) and feed flow rate (Q) on MF performance, 24 experiments have been performed. The highest steady state permeate flux (138.97 dm3/m2h) was achieved for 0.12 MPa and 1000 dm3/h. Fouling analysis has been studied based on the resistance-in series model. It has been found that the percentage of irreversible fouling resistance during the MF increases with increasing TMP and Q. The permeate flux regeneration has been achieved by membrane cleaning with 3 wt % NaOH and 3 wt % H3PO4 at 45 °C. The results of this study are expected to be useful in industrially employing the MF process as the first step of glycerol fermentation broth purification.

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Cross-flow microfiltration of fermentation broth containing native corn starch

Cited by 14 publications

References 41 publications

Lactic acid production in a membrane bioreactor system with thermophilicBacillus coagulans: fouling analysis of the used ceramic membranes

Lactic acid production in a membrane bioreactor system with thermophilicBacillus coagulans: fouling analysis of the used ceramic membranes

Cloning, expression, and purification of insect (Sitophilus oryzae) alpha-amylase, able to digest granular starch, in Yarrowia lipolytica host

Cross-Flow Microfiltration of Glycerol Fermentation Broths with Citrobacter freundii

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