Cross-dressing: an alternative mechanism for antigen presentation

Campana, Stefania; Pasquale, Claudia De; Carrega, Paolo; Ferlazzo, Guido; Bonaccorsi, Irene

doi:10.1016/j.imlet.2015.11.002

Cited by 88 publications

(81 citation statements)

References 75 publications

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“…Moreover, the addition of blocking antibodies to adhesion molecules, such as ICAM‐1 or CD11a, to the co‐culture suppressed the MHC‐II transfer from DCs to basophils, suggesting the importance of cell interaction via adhesion molecules in the MHC‐II transfer. These observations were consistent with the phenomenon known as “trogocytosis” …”

Section: Basophils Can Function As Th2‐oriented Antigen‐presenting Cesupporting

confidence: 89%

Recent advances in understanding basophil‐mediated Th2 immune responses

Yamanishi

Miyake

Iki

et al. 2017

Immunological Reviews

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Basophils, the least common granulocytes, represent only ~0.5% of peripheral blood leukocytes. Because of the small number and some similarity with mast cells, the functional significance of basophils remained questionable for a long time. Recent studies using newly-developed analytical tools have revealed crucial and non-redundant roles for basophils in various immune responses, particularly Th2 immunity including allergy and protective immunity against parasitic infections. In this review, we discuss the mechanisms how basophils mediate Th2 immune responses and the nature of basophil-derived factors involved in them. Activated basophils release serine proteases, mouse mast cell protease 8 (mMCP-8), and mMCP-11, that are preferentially expressed by basophils rather than mast cells in spite of their names. These proteases elicit microvascular hyperpermeability and leukocyte infiltration in affected tissues, leading to inflammation. Basophil-derived IL-4 also contributes to eosinophil infiltration while it acts on tissue-infiltrating inflammatory monocytes to promote their differentiation into M2 macrophages that in turn dampen inflammation. Although basophils produce little or no MHC class II (MHC-II) proteins, they can acquire peptide-MHC-II complexes from dendritic cells via trogocytosis and present them together with IL-4 to naive CD4 T cells, leading to Th2 cell differentiation. Thus, basophils contribute to Th2 immunity at various levels.

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Section: Basophils Can Function As Th2‐oriented Antigen‐presenting Cesupporting

confidence: 89%

Recent advances in understanding basophil‐mediated Th2 immune responses

Yamanishi

Miyake

Iki

et al. 2017

Immunological Reviews

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“…These observations appeared to be consistent with the phenomenon of trogocytosis that has been demonstrated in lymphocytes when interacted with APCs (26,27). In trogocytosis, receptor-ligand interactions are thought to play an important role in the cell-cell interaction to trigger the transfer of proteins (28). Both BMBAs and BMDCs expressed LFA-1 (CD11a/ CD18), Mac-1 (CD11b/CD18), and their ligand ICAM-1 (Fig.…”

Section: Cd11csupporting

confidence: 86%

“…Acquisition of MHC-I by NK cells from tumor cells leads to a reduced NK cytotoxic function (41). Acquisition of MHC-II from DCs via trogocytosis has been shown in CD4 T cells, NK cells, DCs, macrophages, and lymph node stromal cells (28,(42)(43)(44)(45). MHC-II-dressed recipient cells either stimulate or suppress T cells, perhaps depending on the expression and acquisition of costimulatory molecules (28).…”

Section: Cd11cmentioning

confidence: 99%

“…Acquisition of MHC-II from DCs via trogocytosis has been shown in CD4 T cells, NK cells, DCs, macrophages, and lymph node stromal cells (28,(42)(43)(44)(45). MHC-II-dressed recipient cells either stimulate or suppress T cells, perhaps depending on the expression and acquisition of costimulatory molecules (28). Innate lymphoid cell (ILC)2s can also acquire MHC-II from DCs, although ILC2s express endogenous MHC-II, and process and present protein antigens to T cells (46).…”

Section: Cd11cmentioning

confidence: 99%

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Trogocytosis of peptide–MHC class II complexes from dendritic cells confers antigen-presenting ability on basophils

Miyake

Shiozawa

Nagao

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

110

101

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Th2 immunity plays important roles in both protective and allergic responses. Nevertheless, the nature of antigen-presenting cells responsible for Th2 cell differentiation remains ill-defined compared with the nature of the cells responsible for Th1 and Th17 cell differentiation. Basophils have attracted attention as a producer of Th2-inducing cytokine IL-4, whereas their MHC class II (MHC-II) expression and function as antigen-presenting cells are matters of considerable controversy. Here we revisited the MHC-II expression on basophils and explored its functional relevance in Th2 cell differentiation. Basophils generated in vitro from bone marrow cells in culture with IL-3 plus GM-CSF displayed MHC-II on the cell surface, whereas those generated in culture with IL-3 alone did not. Of note, these MHC-II–expressing basophils showed little or no transcription of the corresponding MHC-II gene. The GM-CSF addition to culture expanded dendritic cells (DCs) other than basophils. Coculture of basophils and DCs revealed that basophils acquired peptide–MHC-II complexes from DCs via cell contact-dependent trogocytosis. The acquired complexes, together with CD86, enabled basophils to stimulate peptide-specific T cells, leading to their proliferation and IL-4 production, indicating that basophils can function as antigen-presenting cells for Th2 cell differentiation. Transfer of MHC-II from DCs to basophils was also detected in draining lymph nodes of mice with atopic dermatitis-like skin inflammation. Thus, the present study defined the mechanism by which basophils display MHC-II on the cell surface and appears to reconcile some discrepancies observed in previous studies.

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“…TNTs have been shown to establish cytoplasmic bridges between variety of immune cells including human peripheral blood natural killer (NK) cells, EBV-transformed B-cells and the macrophages (Onfelt et al, 2004). Indeed, TNT formation in the context of immunity and inflammation such as antigen presentation (MHC complexes) has been widely reported in recent years (Chinnery et al, 2008; Schiller et al, 2013b; Seyed-Razavi et al, 2013; Campana et al, 2015; Osteikoetxea-Molnar et al, 2016). Arguably, such functional connectivity between immune cells may circumvent host defense against pathogens (Watkins and Salter, 2005; Zaccard et al, 2016).…”

Section: Tnts and Evs: Roles In Immunoregulation And Inflammatory Resmentioning

confidence: 99%

Extracellular Vesicles, Tunneling Nanotubes, and Cellular Interplay: Synergies and Missing Links

Nawaz

Fatima

2017

Front. Mol. Biosci.

103

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The process of intercellular communication seems to have been a highly conserved evolutionary process. Higher eukaryotes use several means of intercellular communication to address both the changing physiological demands of the body and to fight against diseases. In recent years, there has been an increasing interest in understanding how cell-derived nanovesicles, known as extracellular vesicles (EVs), can function as normal paracrine mediators of intercellular communication, but can also elicit disease progression and may be used for innovative therapies. Over the last decade, a large body of evidence has accumulated to show that cells use cytoplasmic extensions comprising open-ended channels called tunneling nanotubes (TNTs) to connect cells at a long distance and facilitate the exchange of cytoplasmic material. TNTs are a different means of communication to classical gap junctions or cell fusions; since they are characterized by long distance bridging that transfers cytoplasmic organelles and intracellular vesicles between cells and represent the process of heteroplasmy. The role of EVs in cell communication is relatively well-understood, but how TNTs fit into this process is just emerging. The aim of this review is to describe the relationship between TNTs and EVs, and to discuss the synergies between these two crucial processes in the context of normal cellular cross-talk, physiological roles, modulation of immune responses, development of diseases, and their combinatory effects in tissue repair. At the present time this review appears to be the first summary of the implications of the overlapping roles of TNTs and EVs. We believe that a better appreciation of these parallel processes will improve our understanding on how these nanoscale conduits can be utilized as novel tools for targeted therapies.

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Cross-dressing: an alternative mechanism for antigen presentation

Cited by 88 publications

References 75 publications

Recent advances in understanding basophil‐mediated Th2 immune responses

Recent advances in understanding basophil‐mediated Th2 immune responses

Trogocytosis of peptide–MHC class II complexes from dendritic cells confers antigen-presenting ability on basophils

Extracellular Vesicles, Tunneling Nanotubes, and Cellular Interplay: Synergies and Missing Links

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