Cross-amplification of nuclear microsatellite markers in two species of Cryptanthus Otto & A. Dietr. (Bromeliaceae)

Abstract

Thirty-eight nuclear microsatellite loci originally developed for Aechmea caudata Lindm., Orthophytum ophiuroides Louzada & Wand., Pitcairnia albiflos Herb., Vriesea gigantea (Gaud.) and V. simplex (Vell.) Beer were tested in Cryptanthus burle-marxii Leme and C. zonatus (Vis.) Vis. Of the 38 loci tested, 13 were polymorphic. Ten polymorphic microsatellite loci were selected to be amplified and genotyped in one population each of C. burle-marxii and C. zonatus. The observed and expected heterozygosity per locus in the C. burle-marxii population ranged from 0.050 to 0.850 and 0.050 to 0.770, respectively. In C. zonatus, the observed and expected heterozygosity per locus ranged from 0.167 to 0.846 and 0.290 to 0.692, respectively. The O. ophiuroides locus Op52 for the C. zonatus population and P. albiflos locus PaC05 for the two species showed significant departure from HWE. These ten polymorphic loci tested will be used to assess the genetic diversity and structure of the two species of Cryptanthus.

Transferability of nuclear microsatellite markers to the atmospheric bromeliads Tillandsia recurvata and T. aeranthos (Bromeliaceae)

“…For T. aeranthos, we successfully cross-amplified nine microsatellite loci, seven of them being polymorphic with a scorable pattern (Table 1). The percentages of successful amplification and polymorphic loci (81.2 and 38.9% in T. recurvata, and 60 and 46.7% in T. aeranthos, respectively) are in accordance with other studies with monocots ( Barbará et al 2007) and other bromeliads (e.g., Palma-Silva et al 2007;Wöhrmann and Weising 2011;de Miranda et al 2012;Goetze et al 2013;Ferreira et al 2017).…”

Transferability of nuclear microsatellite markers to Stigmatodon species: a bromeliad genus endemic to vertical cliffs of inselbergs in Brazil

“…Accordingly, the level of transferability obtained for the species studied here can be considered above average, since 12 of the 24 pairs of primers tested (50%) yielded positive results. Similarly successful results in marker transfer have also been reported for other species of Bromeliaceae (Barbará et al 2007a;Paggi et al 2008;Palma-Silva et al 2009Wöhrmann and Weising 2011;Zanella et al 2012;Goetze et al 2013;Lavor et al 2014;Neri et al 2015;Ferreira et al 2017;Pereira et al 2017;Chaves et al 2018;Godoy et al 2018Godoy et al , 2019Zenk et al 2018). High transferability between species of the same Bromeliaceae subfamily is probably due to the family's large adaptive radiation, leading to low levels of divergence in their DNA sequences (see Palma-Silva et al 2006;Barbará et al 2007b).…”