Crop nitrogen use efficiency for sustainable food security and climate change mitigation

Madan, Bhumika; Malik, Aakansha; Raghuram, Nandula

doi:10.1016/b978-0-12-822916-3.00003-2

Cited by 10 publications

(12 citation statements)

References 62 publications

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“…Transporters are known to regulate N-response/NUE in many crops ( Mandal et al, 2018 ; Raghuram and Sharma, 2019 ; Madan et al, 2022 ), including rice ( Pathak et al, 2020 ; Kumari et al, 2021 ; Nazish et al, 2021 ; Mandal et al, 2022 ). But they were not validated in contrasting genotypes to the best of our knowledge.…”

Section: Discussionmentioning

confidence: 99%

“…Plant biology has a central role in understanding and improving crop NUE ( Raghuram and Sharma, 2019 ; Udvardi et al, 2021 ; Madan et al, 2022 ). This has to begin with cereals that dominate global crop production and fertilizer demand, of which rice is predominant, due to its lowest NUE ( Norton et al, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

“…All other metabolites containing N are generated by transamination of amino acids, which also provide the main organic N-pool for translocation and secondary remobilization during senescence, which is particularly important in cereals ( Snyder and Tegeder, 2021 ). All these processes have been targeted for understanding and improving NUE with varying results ( Mandal et al, 2018 ; Raghuram and Sharma, 2019 ; Sinha et al, 2016 , 2020 ; Madan et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

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Comparative Transcriptomic Analyses of Nitrate-Response in Rice Genotypes With Contrasting Nitrogen Use Efficiency Reveals Common and Genotype-Specific Processes, Molecular Targets and Nitrogen Use Efficiency-Candidates

et al. 2022

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The genetic basis for nitrogen (N)-response and N use efficiency (NUE) must be found in N-responsive gene expression or protein regulation. Our transcriptomic analysis of nitrate response in two contrasting rice genotypes of Oryza sativa ssp. Indica (Nidhi with low NUE and Panvel1 with high NUE) revealed the processes/functions underlying differential N-response/NUE. The microarray analysis of low nitrate response (1.5 mM) relative to normal nitrate control (15 mM) used potted 21-days old whole plants. It revealed 1,327 differentially expressed genes (DEGs) exclusive to Nidhi and 666 exclusive to Panvel1, apart from 70 common DEGs, of which 10 were either oppositely expressed or regulated to different extents. Gene ontology analyses revealed that photosynthetic processes were among the very few processes common to both the genotypes in low N response. Those unique to Nidhi include cell division, nitrogen utilization, cytoskeleton, etc. in low N-response, whereas those unique to Panvel1 include signal transduction, protein import into the nucleus, and mitochondria. This trend of a few common but mostly unique categories was also true for transporters, transcription factors, microRNAs, and post-translational modifications, indicating their differential involvement in Nidhi and Panvel1. Protein-protein interaction networks constructed using DEG-associated experimentally validated interactors revealed subnetworks involved in cytoskeleton organization, cell wall, etc. in Nidhi, whereas in Panvel1, it was chloroplast development. NUE genes were identified by selecting yield-related genes from N-responsive DEGs and their co-localization on NUE-QTLs revealed the differential distribution of NUE-genes between genotypes but on the same chromosomes 1 and 3. Such hotspots are important for NUE breeders.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Comparative Transcriptomic Analyses of Nitrate-Response in Rice Genotypes With Contrasting Nitrogen Use Efficiency Reveals Common and Genotype-Specific Processes, Molecular Targets and Nitrogen Use Efficiency-Candidates

et al. 2022

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“…Engineering any multi-genic trait through transgenics can be a challenge, unless one or a few genes account for most of the potential for its improvement. Efforts for transgenic improvement of NUE over the last three decades have met with limited success, mostly due to the trial and error approach in the choice of genes (Raghuram and Sharma, 2019 ; Madan et al, 2022 ). However, things seem to be improving, as Chen et al show here that the co-overexpression of OsNAR2.1 and OsNRT2.3a could increase rice yield, N transport efficiency, N recovery efficiency, and agronomic N use efficiency in rice.…”

Section: From Transgenic To Genome Editing For Nuementioning

confidence: 99%

Editorial: Nitrogen Use Efficiency and Sustainable Nitrogen Management in Crop Plants

et al. 2022

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“…Sustainable nitrogen (N) management is crucial for sustainable food systems and mandated by two UN resolutions (UNEP/EA.4/Res.14 and UNEP/EA.5/Res.2), to prevent N-pollution and its impacts on health, biodiversity and climate change (Abrol et al, 2017, Sutton et al, 2019, 2020, 2021, Raghuram et al, 2021). Plant biologists have a major role in improving crop nitrogen use efficiency to minimize fertilizer wastage and pollution (Mandal et al, 2018, Raghuram and Sharma 2019, Udvardi et al, 2021, Madan et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

Genomewide basis for nitrogen use efficiency in contrasting genotypes of rice

Sharma

Jaiswal

Kumari

et al. 2022

Preprint

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Rice is an ideal crop with huge germplasm diversity and post-genomic resources for improvement of nitrogen (N) use efficiency (NUE). There is a paucity of comparative studies on rice genotypes contrasting for NUE, especially with urea, the predominant fertilizer in rice growing countries. In this study, low urea-responsive transcriptomes of contrasting rice genotypes namely Nidhi (low NUE) and Panvel1 (high NUE) were compared. They were based on whole plants grown for 21 days in pots containing nutrient-depleted soil fertilized with normal (15 mM) and low urea (1.5 mM) media. There were 1497 and 2819 differentially expressed genes (DEGs) in Nidhi and Panvel1, respectively, of which 271 were common. Though 1226 DEGs were genotype-specific in Nidhi and 2548 in Panvel1, there was far higher commonality in underlying processes. High NUE is associated with the urea-responsive regulation of other nutrient transporters, miRNAs, transcription factors and better photosynthesis, water use efficiency and post translational modifications. Many of their genes co-localized to NUE QTLs on chromosomes 1, 3 and 9. Field evaluation of the contrasting genotypes under different doses of urea revealed better performance of Panvel1 in different agronomic parameters including grain yield, transport/uptake efficiencies and NUE. Comparison of our urea-based transcriptomes with our previous nitrate-based transcriptomes from the same contrasting rice genotypes revealed many common processes despite large differences in their expression profiles. Our model proposes that differential involvement of transporters and transcription factors among others contributes to better urea uptake, translocation, utilization, flower development and yield for high NUE.SummaryRice genotypes with contrasting urea use efficiency differ in the role of transporters, transcription factors, miRNAs, post-translational modifications, photosynthesis and water use efficiency

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Crop nitrogen use efficiency for sustainable food security and climate change mitigation

Cited by 10 publications

References 62 publications

Comparative Transcriptomic Analyses of Nitrate-Response in Rice Genotypes With Contrasting Nitrogen Use Efficiency Reveals Common and Genotype-Specific Processes, Molecular Targets and Nitrogen Use Efficiency-Candidates

Comparative Transcriptomic Analyses of Nitrate-Response in Rice Genotypes With Contrasting Nitrogen Use Efficiency Reveals Common and Genotype-Specific Processes, Molecular Targets and Nitrogen Use Efficiency-Candidates

Editorial: Nitrogen Use Efficiency and Sustainable Nitrogen Management in Crop Plants

Genomewide basis for nitrogen use efficiency in contrasting genotypes of rice

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