Crop breeding for salt tolerance in the era of molecular markers and marker‐assisted selection

Ashraf, Muhammad; Foolad, Majid R.

doi:10.1111/pbr.12000

Cited by 183 publications

(116 citation statements)

References 132 publications

(116 reference statements)

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“…Thus, proline content in plant tissues was used as a marker-assisted selection molecule in some breeding programs that endeavored to improve salinity and drought tolerance in plants (Flowers and Flowers, 2005;Ma et al, 2007;Ashraf and Foolad, 2013;Wu et al, 2013). In the present study, I determined whether proline accumulation in the date palm tree was specifically correlated to drought, and high salinity conditions, or was a general response to abiotic stresses.…”

Section: Introductionmentioning

confidence: 97%

Short Communication Proline accumulation is a general response to abiotic stress in the date palm tree (Phoenix dactylifera L.)

Yaish¹

2015

Genet. Mol. Res.

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ABSTRACT. Plants exposed to certain abiotic stress conditions tend to produce the amino acid proline, which acts as an active osmolyte, a metal chelator, an antioxidant, and a signaling molecule. There is increasing evidence that proline accumulates in plants due to a wide range of abiotic stress, in particular high soil salinity and drought. Therefore, proline content is often used as a marker-assisted breeding tool aimed at improving drought and salinity tolerance. In this study, it was investigated whether proline accumulation in date palm (Phoenix dactylifera L.) seedlings occurs solely due to high salinity and drought stresses or due to other unspecified abiotic stresses, including salinity and salinity shock, drought, extreme temperatures, and abscisic acid. The free proline assays revealed that this amino acid over-accumulated in the roots and leaves of each stresstreated plant, and was remarkably high when leaves were exposed to suboptimum temperatures and salinity stress. These results indicate that the production of proline is a common response to various abiotic stresses and its differential accumulation cannot be used as a molecular marker in date palm breeding programs aimed at improving drought or salinity 9943-9950 (2015) tolerance traits in date palms. This conclusion is consistent with the theory that the molecular outcomes of abiotic stresses are often non-specific.

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Section: Introductionmentioning

confidence: 97%

Short Communication Proline accumulation is a general response to abiotic stress in the date palm tree (Phoenix dactylifera L.)

Yaish¹

2015

Genet. Mol. Res.

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“…In the last few years, several molecular markers have been recognized that can scrutinize complex traits into individual components. Marker assisted breeding is one of the significant molecular approaches which accelerates and aids conventional breeding (Ashraf & Foolad, 2013). Application of SSRs in V. radiata and V. umbellata includes determining the identification of hybrids, phylogeny and gene mapping establishing marker-trait association using segregating populations (Michelmore et al, 1991).…”

Section: Introductionmentioning

confidence: 99%

Assessment of Genetic Purity of Inter-Specific F1 Hybrids Involving Vigna Radiata and Vigna Umbellata

Bhanu¹,

Kumar²,

Singh³

et al. 2017

JEBAS

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Genetic purity test of true hybrids from controlled crosses before further generations of selfing or crossing and selection is essential for Mungbean improvement. The present study was conducted to transfer mungbean yellow mosaic (MYM) disease resistance in mungbean from ricebean and to assess the genetic purity of developed inter-specific F1 hybrids using morphological features and microsatellite markers. Inter-specific crosses were made involving two genotypes of mungbean viz., K 851 and TM 96-2 and one genotype of ricebean (RBL 1) where the crossability results revealed significant differences. Crossability was recorded 8.2% (TM 96-2 × RBL 1) and 4.6% (K 851 × RBL 1). Pollen fertility was recorded 1.6% and 3.4% in TM 96-2 × RBL 1 and K 851 × RBL 1, respectively. Morphological features such as epicotyl colour, hypocotyl length, petiole length, germination habit, etc., were used as indicators of true hybridity. Further, the microsatellite markers were used to confirm the genetic purity of the developed inter-specific hybrid. These hybrids exhibited resistance against mungbean yellow mosaic disease under natural epiphytotic field conditions. The present study will be useful in developing high yielding varieties or lines of mungbean coupled with stable MYMV resistance.

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“…Instead of directly selecting for the phenotype, selection takes place for the linked marker; in a later step, the presence of the phenotype is then verified. This procedure enables the selection process to proceed in the absence of conditions promoting the phenotype for example, low heritability or absence of the pathogen or pest; (Collard and Mackill, 2008) or when phenotypic screening is cumbersome for example, salt tolerance (Ashraf and Foolad, 2013).…”

Section: Introductionmentioning

confidence: 99%

Alternative markers linked to the Phg-2 angular leaf spot resistance locus in common bean using the Phaseolus genes marker database

Miller¹,

Gepts²,

Kimno³

et al. 2018

Afr. J. Biotechnol.

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Common bean (Phaseolus vulgaris) is a rich source of protein, vitamins, and micronutrients and is an important crop for food security throughout Latin America, Asia, and Africa. Among tropical and subtropical regions of the world, where the majority of beans are grown, yield losses due to the pathogenic fungus Pseudocercospora griseola, causing angular leaf spot (ALS), can be as high as 80%. The strategic use of marker-assisted selection (MAS) to pyramid multiple resistance genes into a single genetic background with preferred morphological and cooking characteristics is being implemented by six research groups throughout East Africa that make up the African Bean Consortium (ABC). Identifying unique markers that are polymorphic among multiple parents is a major source of marker attrition. In this study, an illustration of how 22 DNA sequences physically linked to the Phg-2 ALS resistance locus were identified using the PhaseolusGenes marker database and checked for amplification and polymorphism among 16 ABC breeding parents are given. Only three polymorphic markers could be identified following this procedure; one (g796), showed a polymorphism present only in the ALS resistance donor, Mexico 54. The PCR protocol developed to identify the g796 polymorphism was validated among five laboratories. Furthermore, co-segregation analysis of the marker and ALS resistance phenotype in a population of 100 F 2 individuals from the cross between French bean (that is, green bean) genotype Amy and ALS resistance donor Mexico 54 showed the marker is genetically linked (3 cM) to the Phg-2 locus, in addition to being physically linked. This study suggests that in the near future, genetic resequencing data of diverse common bean accessions, compiled within an easily accessible database, will facilitate identification of markers for MAS, marker/trait association, and candidate gene identification.

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Crop breeding for salt tolerance in the era of molecular markers and marker‐assisted selection

Cited by 183 publications

References 132 publications

Short Communication Proline accumulation is a general response to abiotic stress in the date palm tree (Phoenix dactylifera L.)

Short Communication Proline accumulation is a general response to abiotic stress in the date palm tree (Phoenix dactylifera L.)

Assessment of Genetic Purity of Inter-Specific F1 Hybrids Involving Vigna Radiata and Vigna Umbellata

Alternative markers linked to the Phg-2 angular leaf spot resistance locus in common bean using the Phaseolus genes marker database

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