Cronobacter spp., foodborne pathogens threatening neonates and infants

Chen, Qiming; Zhu, Yan; Qin, Zhen; Qiu, Yongjun; Zhao, Liming

doi:10.15302/j-fase-2018208

Cited by 5 publications

(7 citation statements)

References 110 publications

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“…Among the upregulated genes, the 6-phospho-glucosidase gene glvA was the most significantly upregulated by 5.24-fold (Supplementary Tables 1, 3, 4). 6-phosphoglucosidase has oxidoreductase activity, acting on CH-OH donors, NAD or NADP as receptors, and can bind to metal ions to express corresponding functions (Chen et al, 2018). The differential gene expression of the SdiA gene deletion strain was markedly downregulated, which involved the VI secretion system and ABC transport system (Papenfort and Bassler, 2016).…”

Section: Lack Of Sdia Alters the Expression Of Genes Involved In Moti...mentioning

confidence: 99%

“…In the downregulated gene expression, the key function of the VI secretory system was the ATP-active membrane-associated protein VasK, whose gene expression was downregulated by 1.53-fold. The type VI secretion system plays an important role in the cell adhesion, virulence, invasion and proliferation of C. sakazakii; this system also produces protein toxin, which is a potential toxic factor (Chen et al, 2018). Moreover, the expression level of related genes in the ABC transport system was downregulated by 1.5-fold, which may affect the pathogenesis and virulence of C. sakazakii and reduce its ability to infect host cells and bacteria in drug resistance (Supplementary Tables 2-4) (Wichelecki et al, 2015;Christoph and Robert, 2018).…”

Section: Lack Of Sdia Alters the Expression Of Genes Involved In Moti...mentioning

confidence: 99%

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SdiA Enhanced the Drug Resistance of Cronobacter sakazakii and Suppressed Its Motility, Adhesion and Biofilm Formation

et al. 2022

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Cronobacter sakazakii is a common foodborne pathogen, and the mortality rate of its infection is as high as 40–80%. SdiA acts as a quorum sensing regulator in many foodborne pathogens, but its role in C. sakazakii remains unclear. Here, we further determined the effect of the sdiA gene in C. sakazakii pathogenicity. The SdiA gene in C. sakazakii was knocked out by gene editing technology, and the biological characteristics of the ΔsdiA mutant of C. sakazakii were studied, followed by transcriptome analysis to elucidate its effects. The results suggested that SdiA gene enhanced the drug resistance of C. sakazakii but diminished its motility, adhesion and biofilm formation ability and had no effect on its growth. Transcriptome analysis showed that the ΔsdiA upregulated the expression levels of D-galactose operon genes (including dgoR, dgoK, dgoA, dgoD and dgoT) and flagella-related genes (FliA and FliC) in C. sakazakii and downregulated the expression levels of related genes in the type VI secretion system (VasK gene was downregulated by 1.53-fold) and ABC transport system (downregulated by 1.5-fold), indicating that SdiA gene was related to the physiological metabolism of C. sakazakii. The results were useful for clarifying the pathogenic mechanism of C. sakazakii and provide a theoretical basis for controlling bacterial infection.

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Section: Lack Of Sdia Alters the Expression Of Genes Involved In Moti...mentioning

confidence: 99%

Section: Lack Of Sdia Alters the Expression Of Genes Involved In Moti...mentioning

confidence: 99%

SdiA Enhanced the Drug Resistance of Cronobacter sakazakii and Suppressed Its Motility, Adhesion and Biofilm Formation

et al. 2022

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“…Among the upregulated genes, the 6-phospho-glucosidase gene glvA was the most significantly upregulated by 5.24-fold (Tables S1, S3 and S4). 6-Phosphoglucosidase has oxidoreductase activity, acting on CH-OH donors, NAD or NADP as receptors, and can bind to metal ions to express corresponding functions (31).…”

Section: Lack Ofmentioning

confidence: 99%

“…In the downregulated gene expression, the key function of the VI secretory system was the ATP-active membrane-associated protein VasK, whose gene expression was downregulated by 1.53-fold. The type VI secretion system plays an important role in the cell adhesion, virulence, invasion and proliferation of C. sakazakii; this system also produces protein toxin, which is a potential toxic factor (31). Moreover, the expression level of related genes in the ABC transport system was downregulated by 1.5-fold, which may affect the pathogenesis and virulence of C. sakazakii and reduce its ability to infect host cells and bacteria in drug resistance (Tables S2, S3 and S4) (29,30).…”

Section: Lack Of Sdia Alters the Expression Of Genes Involved In Moti...mentioning

confidence: 99%

SdiA, a Quorum Sensing Transcriptional Regulator, Enhanced the Drug Resistance of Cronobacter sakazakii and Suppressed its Motility, Adhesion and Biofilm Formation

Cheng¹,

Yan²,

Liu³

et al. 2022

Preprint

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Cronobacter sakazakii is a common foodborne pathogen, and the mortality rate of its infection is as high as 40-80%. Quorum sensing is a regulation system of bacterial density-dependent multigene expression and is an important regulatory mechanism involved in adhesion, biofilm formation and virulence. C. sakazakii contains a QS signal molecular receiver, which is the LuxR receptor homolog SdiA, but its regulatory mechanism in C. sakazakii QS has not been defined. Here, we further determined the effect of SdiA on the QS system of C. sakazakii. The SdiA gene in C. sakazakii was knocked out by gene editing technology, and the biological characteristics of the ΔsdiA gene deletion strain of C. sakazakii were studied, followed by transcriptome analysis to elucidate its effects. The results suggested that SdiA enhanced the drug resistance of C. sakazakii but diminished its motility, adhesion and biofilm formation ability and had no effect on its growth. Transcriptome analysis showed that the deletion of the SdiA gene upregulated the expression levels of D-galactose operon genes (including dgoR, dgoK, dgoA, dgoD and dgoT) and flagella-related genes (FliA and FliC) in C. sakazakii and downregulated the expression levels of related genes in the type VI secretion system (VasK gene was downregulated by 1.53-fold) and ABC transport system (downregulated by 1.5-fold), indicating that SdiA was related to the physiological metabolism of C. sakazakii. The results of this study may be useful for clarifying the pathogenic mechanism of C. sakazakii and provide a theoretical basis for controlling bacterial infection.

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“…Because of its polysaccharide capsule, resistance to desiccation, and ability to produce a yellow carotenoid pigment that protects it from oxygen radicals, Cronobacter sakazakii may be found in a range of environments (Iversen and Forsythe, 2003). Cronobacter sakazakii infection usually attacks all age groups, especially prematurely born neonates and infants, via consuming contaminated powdered infant formula (MF) and milk powder, causing bacteremia, sepsis, necrotizing enterocolitis, meningitis and acute diarrhea, with a high mortality rate of up to 80% (Chen et al, 2018;Kadlicekova et al, 2018;Henry and Fouladkhah, 2019). Polymerase chain reaction (PCR) and other genotyping techniques have been seen as useful methods for performing epidemiological monitoring to determine the similarity of bacterial pathogens isolated from dietary, environmental, and clinical samples.…”

Section: Introductionmentioning

confidence: 99%

Isolation and Molecular identification of Cronobacter sakazakii isolated from different foodstuffs and infant stools

Abuelsoud,

Rizk,

Maarouf

2023

Benha Veterinary Medical Journal

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Cronobacter sakazakii is a foodborne pathogen that can cause serious infections (enterocolitis and meningitis) with high fatalities in neonatal infants. This work was designed to isolate Cronobacter sakazakii and explore its molecular identification and prevalence rate. The present study was performed on 225 random samples of powdered milk infant formula, milk powder, minced meat, fresh cow milk, and diarrheic infant stool (45 for each) collected from different shops and hospitals in Cairo, Kaliobia, and Monofia Governorates, Egypt. Cronobacter sakazakii isolates were characterized by conventional phenotypical methods and MICROBACT TM kits, then genotypic identification of the common 16S rRNA gene by PCR. Out of the fifteen Cronobacter sakazakii isolates obtained, the majority were isolated from powdered milk infant formula samples 5 isolates (11.1%), 4 from milk powder (8.9%), 3 from diarrheic infant stool (6.7%), 2 from minced meat (4.4%), and 1 from fresh cow milk samples (2.2%). The Cronobacter sakazakii 16S rRNA gene was detected in all tested isolates. Therefore, the results of this work highlight the importance of Cronobacter sakazakii as a high public health concern in newborns, indicating the potential risk of infant infection by this bacteria from mostly all sources of infant food.

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Cronobacter spp., foodborne pathogens threatening neonates and infants

Cited by 5 publications

References 110 publications

SdiA Enhanced the Drug Resistance of Cronobacter sakazakii and Suppressed Its Motility, Adhesion and Biofilm Formation

SdiA Enhanced the Drug Resistance of Cronobacter sakazakii and Suppressed Its Motility, Adhesion and Biofilm Formation

SdiA, a Quorum Sensing Transcriptional Regulator, Enhanced the Drug Resistance of Cronobacter sakazakii and Suppressed its Motility, Adhesion and Biofilm Formation

Isolation and Molecular identification of Cronobacter sakazakii isolated from different foodstuffs and infant stools

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