Cronobacter condimenti sp. nov., isolated from spiced meat, and Cronobacter universalis sp. nov., a species designation for Cronobacter sp. genomospecies 1, recovered from a leg infection, water and food ingredients

Abstract

A re-evaluation of the taxonomic position of five strains, one assigned to Cronobacter sakazakii (strain 1330T, isolated from spiced meat purchased in Slovakia), two previously assigned to Cronobacter genomospecies 1 (strains NCTC 9529T and 731, isolated from water and a leg infection, respectively) and two previously assigned to Cronobacter turicensis (strains 96 and 1435, isolated from onion powder and rye flour, respectively) was carried out. The analysis included phenotypic characterization, 16S rRNA gene sequencing and multilocus sequence analysis (MLSA) of seven housekeeping genes (atpD, fusA, glnS, gltB, gyrB, infB, ppsA; 3036 bp). 16S rRNA gene sequence analysis and MLSA showed that strain 1330T formed an independent phylogenetic lineage in the MLSA, with Cronobacter dublinensis LMG 23823T as the closest neighbour. DNA–DNA reassociation and phenotypic analysis revealed that strain 1330T represented a novel species, for which the name Cronobacter condimenti sp. nov. is proposed (type strain 1330T = CECT 7863T = LMG 26250T). Strains NCTC 9529T, 731, 96 and 1435 clustered together within an independent phylogenetic lineage, with C. turicensis LMG 23827T as the closest neighbour in the MLSA. DNA–DNA reassociation and phenotypic analysis confirmed that these strains represent a novel species, for which the name Cronobacter universalis sp. nov. is proposed (type strain NCTC 9529T = CECT 7864T = LMG 26249T).

Occurrence of <i>Cronobacter</i> spp. in Dried Foods, Fresh Vegetables and Soil

“…lausannensis, C. malonaticus, C. muytjensii, C. turicensis and the unnamed genotype species I. Thereafter, C. condimenti and C. univeralis were added in 2012 Joseph et al, 2012, and C. helventicus, C. pulveris and C. zurichensis were added in 2013Brady el al., 2013 . The natural habitat of these bacteria is unknown, but the various growth properties suggests that Cronobacter spp.…”

Cronobacter spp. as emerging causes of healthcare-associated infection

“…63 Pertinent to this, is the recognition of bacterial 31 colonisation of the nasogastric enteral feeding tube, and the isolation of C. sakazakii from tubes from 32 neonates feed breast milk, and (sterile) ready to feed formula. Breast milk can contain the bacterium, 33 and the C. malonaticus type strain (LMG 23826 T ) was isolated from a breast abscess.…”

Identification of the recently described Cronobacter condimenti by an rpoB-gene-based PCR system

“…In a study by Stoop et al (2009), an rpoB-genetargeted PCR assay was developed, which enables the identification of members of the genus Cronobacter to the species level. In a recent study by Joseph et al (2012) an additional species, C. condimenti, was described within the genus Cronobacter, and a novel species designation, C. universalis, was proposed for the formerly named Cronobacter sp.genomospecies 1. However, as of yet, no species-specific identification systems are available for C. condimenti.…”