Cronobacter

Brandão, Marcelo Luiz Lima; Forsythe, Stephan

doi:10.1007/978-3-030-83217-9_3

Cited by 1 publication

(1 citation statement)

References 104 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…2022). Due to the lack of robustness, biochemical kits are no longer recom‐mended for the identification of Cronobacter isolates (Jackson and Forsythe 2016; Anonymous 2017; Brandão and Forsythe 2022).…”

Section: Resultsmentioning

confidence: 99%

Case report of Curtobacterium isolated from a catheter tip sample misidentified as Cronobacter

Braga

Saad

Oliveira

et al. 2022

Letters in Applied Microbiology

View full text Add to dashboard Cite

The Curtobacterium genus is a member of the family Microbacteriaceae, and Curtobacterium species are recognized as plant pathogens. The aim of this study was to investigate a dubious result of species identification for an infection located on a catheter tip of a patient with Covid‐19. A strain isolated from a catheter tip sample, identified by VITEK® 2 as Cronobacter spp., was submitted to polyphasic analysis: Matrix‐Assisted Laser Desorption Ionization‐Time of Flight Mass Spectrometry (MALDI‐TOF MS) using VITEK® MS, real‐time polymerase chain reaction targeting dnaG gene, and 16S rRNA full gene Sanger sequencing analysis for confirmation. The strain presented negative result using qPCR and could not identified by MALDI‐TOF MS. 16S rRNA full gene Sanger sequencing analysis identified the strain as Curtobacterium spp. The Gram‐variable characteristic (Gram‐negative instead of Gram‐positive) of the isolated strain was the responsible for the misidentification by VITEK® 2 and VITEK® MS did not identify the strain. 16S rRNA full gene sequencing analysis identified the strain as Curtobacterium genus, but other complementary techniques are necessary to identify at species level.

show abstract

Section: Resultsmentioning

confidence: 99%