Crohnʼs Disease Mimicking Perforated Appendicitis

Bhattacharya, Sumona; Moradi, Dovid; Bhandari, Bhavik

doi:10.14309/00000434-201710001-02919

Cited by 1 publication

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“…Among them, Bifidobacterium longum ( B. longum ), as one of the initial microorganisms colonize at host intestinal tract and also the predominant taxa of human gastrointestinal tract, exhibits an instrumental role in maintaining normal GI microenvironment [ 11 ]. Mounting animal trails and clinical studies have demonstrated that B. longum can alleviate colitis symptoms and further reduce chronic inflammation [ 12 , 13 ]. Oral supplement of B. longum 51A has been reported to be effectively protect mice from irinotecan-induced intestinal damage [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Bifidobacterium longum SX-1326 ameliorates gastrointestinal toxicity after irinotecan chemotherapy via modulating the P53 signaling pathway and brain-gut axis

Yue,

Zeng,

Wang

et al. 2024

BMC Microbiol

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Background Colorectal cancer (CRC) is a prevalent malignant malignancy affecting the gastrointestinal tract that is usually treated clinically with chemotherapeutic agents, whereas chemotherapeutic agents can cause severe gastrointestinal toxicity, which brings great pain to patients. Therefore, finding effective adjuvant agents for chemotherapy is crucial. Methods In this study, a CRC mouse model was successfully constructed using AOM/DSS, and the treatment was carried out by probiotic Bifidobacterium longum SX-1326 (B. longum SX-1326) in combination with irinotecan. Combining with various techniques of modern biomedical research, such as Hematoxylin and Eosin (H&E), Immunohistochemistry (IHC), Western blotting and 16S rDNA sequencing, we intend to elucidate the effect and mechanism of B. longum SX-1326 in improving the anticancer efficacy and reducing the side effects on the different levels of molecules, animals, and bacteria. Results Our results showed that B. longum SX-1326 enhanced the expression of Cleaved Caspase-3 (M vs. U = p < 0.01) and down-regulated the expression level of B-cell lymphoma-2 (Bcl-2) through up-regulation of the p53 signaling pathway in CRC mice, which resulted in an adjuvant effect on the treatment of CRC with irinotecan. Moreover, B. longum SX-1326 was also able to regulate the gut-brain-axis (GBA) by restoring damaged enterochromaffin cells, reducing the release of 5-hydroxytryptamine (5-HT) in brain tissue (I vs. U = 89.26 vs. 75.03, p < 0.05), and further alleviating the adverse effects of nausea and vomiting. In addition, B. longum SX-1326 reversed dysbiosis in CRC model mice by increasing the levels of Dehalobacterium, Ruminnococcus, and Mucispirillum. And further alleviated colorectal inflammation by downregulating the TLR4/MyD88/NF-κB signaling pathway. Conclusions In conclusion, our work reveals that B. longum SX-1326 has a favorable effect in adjuvant irinotecan for CRC and amelioration of post-chemotherapy side effects, and also provides the theoretical basis and data for finding a safe and efficient chemotherapeutic adjuvant.

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Section: Introductionmentioning

confidence: 99%