Crocetin ester improves myocardial ischemia via Rho/ROCK/NF-κB pathway

Huang, Zhiheng; Chen, Nan; Wang, Hanqing; Su, Qiang; Xue, Wenhao; Chen, Yanyan; Shan, Xin; Duan, Jin‐Ao; Chen, Gang; Tao, Weiwei

doi:10.1016/j.intimp.2016.05.025

Cited by 25 publications

(20 citation statements)

References 44 publications

(43 reference statements)

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“…Myocardial ischemia refers to the reduction of blood perfusion in the heart, resulting in reduction of heart oxygenation and abnormal myocardial energy metabolism, so that the normal functioning of the heart cannot be well supported (2,3). Essential to myocardial cell activity, oxygen is transported to cells through blood.…”

Section: Introductionmentioning

confidence: 99%

CIRBP protects H9C2 cells against myocardial ischemia through inhibition of NF-κB pathway

Long

Jiang

Feng

et al. 2017

Braz J Med Biol Res

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Myocardial ischemia is a major cause of death and remains a disease with extremely deficient clinical therapies and a major problem worldwide. Cold inducible RNA-binding protein (CIRBP) is reported to be involved in multiple pathological processes, including myocardial ischemia. However, the molecular mechanisms of myocardial ischemia remain elusive. Here, we first overexpressed CIRBP by transfection of pc-CIRBP (pcDNA3.1 containing coding sequenced for CIRBP) and silenced CIRBP by transfection of small interfering RNA targeting CIRBP (siCIRBP). pcDNA3.1 and the negative control of siCIRBP (siNC) were transfected into H9C2 cells to act as controls. We then constructed a cell model of myocardial ischemia through culturing cells in serum-free medium with hypoxia in H9C2 cells. Subsequently, AlamarBlue assay, flow cytometry and western blot analysis were used, respectively, to assess cell viability, reactive oxygen species (ROS) level and apoptosis, and expression levels of IκBα, p65 and Bcl-3. We demonstrated that CIRBP overexpression promoted cell proliferation (P<0.001), inhibited cell apoptosis (P<0.05), reduced ROS level (P<0.001), down-regulated phosphorylated levels of IκBα and p65 (P<0.01 or P<0.001), and up-regulated expression of Bcl-3 (P<0.001) in H9C2 cells with myocardial ischemia. The influence of CIRBP knockdown yielded opposite results. Our study revealed that CIRBP could protect H9C2 cells against myocardial ischemia through inhibition of NF-κB pathway.

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Section: Introductionmentioning

confidence: 99%

CIRBP protects H9C2 cells against myocardial ischemia through inhibition of NF-κB pathway

Long

Jiang

Feng

et al. 2017

Braz J Med Biol Res

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“…In recent years, the uPr system, also mitochondrial uPr, has become a very important cellular adaptive system to regulate the stress-induced signaling pathway, especially in I/R injury (18,30). Based on the beneficial effect of CRO to the heart and previous references (21)(22)(23), it was hypothesized that CRO can regulate the inflammation response and the UPR system.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies have demonstrated that CRO reduced cardiac cytotoxicity and apoptosis by regulating cardiac enzymes and their function (19,20). Additional previous studies demonstrated that cro mechanistically regulated the mitogen associated protein kinase pathway and nuclear factor-κB signaling to alleviate myocardial ischemia injury (21,22). Moreover, CRO significantly reversed spatial learning dysfunction and attenuated hippocampal injury in an in vivo model of vascular dementia, which suggested that CRO exhibits more versatile functions than was previously known (23).…”

Section: Introductionmentioning

confidence: 86%

Crocetin alleviates myocardial ischemia/reperfusion injury by regulating inflammation and the unfolded protein response

et al. 2019

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crocetin, a natural compound, has been demonstrated to exhibit beneficial effects in cardiovascular diseases. Previous studies demonstrated that crocetin reduced ischemia/reperfusion (I/r) injury by attenuating cytotoxicity and cellular apoptosis. However, the previous mechanistic studies did not fully elucidate its pharmacological effects on cardiac damage, especially i/R injury. The present study verified its cardioprotective effects in a Langendorff perfusion system, an ex vivo model of I/R. It was demonstrated that crocetin significantly attenuated the activities of pro-inflammatory cytokines and nuclear factor erythroid-2 related factor 2 (Nrf2)/heme oxygenase-1 signaling. The present study provided novel insight that crocetin regulated the unfolded protein response (UPR) and decreased associated protein levels to protect the heart. Furthermore, it was identified that Nrf2 played a key role in the cardioprotective effect of crocetin by attenuating inflammation and the UPR.

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“…It is well known that the Rho/Rock signal involves a variety of dysfunction. Rho kinase is a small G protein of Rho family serine/threonine kinase, which regulates the biosynthesis of inflammatory cytokines . The key target effector molecule of Rho is rock.…”

Section: Discussionmentioning

confidence: 99%

Protective effect of betulinic acid on Freund's complete adjuvant‐induced arthritis in rats

Ding

Chen

Shen

et al. 2019

J Biochem & Molecular Tox

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The purpose of the experiment was to study the effects of betulinic acid (BA) on adjuvant‐induced arthritis in rats. The rat model of rheumatoid arthritis (AA) was established by Freund's complete adjuvant. Arthritis index, joint pathology, toe swelling, hemorheology, related cytokines and ROCK/NF‐κB signaling pathway were measured in rats. BA can significantly inhibit the arthritis index, improve joint pathology, reduce toe swelling, improve blood rheology, improve synovial cell apoptosis, and restore related cytokine negative regulation of ROCK/NF‐κB signaling pathways. BA has an obvious therapeutic effect on joint inflammation of toes in AA model rats, which may be due to the regulation of ROCK/NF‐κB signaling pathway.

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Crocetin ester improves myocardial ischemia via Rho/ROCK/NF-κB pathway

Cited by 25 publications

References 44 publications

CIRBP protects H9C2 cells against myocardial ischemia through inhibition of NF-κB pathway

CIRBP protects H9C2 cells against myocardial ischemia through inhibition of NF-κB pathway

Crocetin alleviates myocardial ischemia/reperfusion injury by regulating inflammation and the unfolded protein response

Protective effect of betulinic acid on Freund's complete adjuvant‐induced arthritis in rats

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