Crocetin ameliorates non-alcoholic fatty liver disease by modulating mitochondrial dysfunction in L02 cells and zebrafish model

Xu, Zijin; Lin, Susu; Tong, Zheren; Chen, Suhong; Cao, Yifeng; Li, Qiaoqiao; Jiang, Yuli; Cai, Weiguo; Tong, Yingpeng; Bathaie, S. Zahra; Wang, Ping

doi:10.1016/j.jep.2021.114873

Cited by 12 publications

(4 citation statements)

References 37 publications

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“…CRCs at doses of 0.078–0.156 mg/mL protected primary retinal cell cultures, containing a mixture of retinal cells enriched in photoreceptors, Müller and bipolar cells, against blue or white light–mediated apoptosis [ 93 ]. In normal human liver cell lines (L02 cells) CRT doses up to 13.13 μg/mL did not affect cell viability after 48 h of treatment and restored mitochondrial morphology and function in cells with FFA (free fatty acid)-induced lipid accumulation [ 94 ]. CRCs in a dose up to 0.977 mg/mL on human keratinocytes NHEKs (CC-2507) and dermal fibroblasts (C-013-5C) exhibited strong antioxidant capacity, comparable to vitamin E and higher than vitamin C, protecting against UVA-induced peroxidation and demonstrated significant anti-inflammatory properties [ 95 ].…”

Section: Discussionmentioning

confidence: 99%

An In Vitro Study of Saffron Carotenoids: The Effect of Crocin Extracts and Dimethylcrocetin on Cancer Cell Lines

et al. 2022

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Crocus sativus L. has various pharmacological properties, known for over 3600 years. These properties are attributed mainly to biologically active substances, which belong to the terpenoid group and include crocins, picrocrocin and safranal. The aim of the current work was to examine the effects of crocins (CRCs) and their methyl ester derivate dimethylcrocetin (DMCRT) on glioblastoma and rhabdomyosarcoma cell lines, in terms of cytotoxicity and gene expression, implicated in proapoptotic and cell survival pathways. Cell cytotoxicity was assessed with Alamar Blue fluorescence assay after treatment with saffron carotenoids for 24, 48 and 72 h and concentrations ranging from 22.85 to 0.18 mg/mL for CRCs and 11.43 to 0.09 mg/mL for DMCRT. In addition, BAX, BID, BCL2, MYCN, SOD1, and GSTM1 gene expression was studied by qRT-PCR analysis. Both compounds demonstrated cytotoxic effects against glioblastoma and rhabdomyosarcoma cell lines, in a dose- and time-dependent manner. They induced apoptosis, via BAX and BID upregulation, MYCN and BCL-2, SOD1, GSTM1 downregulation. The current research denotes the possible anticancer properties of saffron carotenoids, which are considered safe phytochemicals, already tested in clinical trials for their health promoting properties.

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Section: Discussionmentioning

confidence: 99%

An In Vitro Study of Saffron Carotenoids: The Effect of Crocin Extracts and Dimethylcrocetin on Cancer Cell Lines

et al. 2022

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“…Another round of PBS washing was performed before observation under a stereomicroscope (Ming Mei SZX7; Ming Mei, Guangzhou, China). The oil red O staining area in the liver was quantified using ImageJ 9.0 software [43] in a double-blinded manner.…”

Section: Methodsmentioning

confidence: 99%

Lian-Mei-Yin formula alleviates diet-induced hepatic steatosis by suppressing Yap1/FOXM1 pathway-dependent lipid synthesis

Zhang,

Cao,

Liang

et al. 2024

ABBS

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Non-alcoholic fatty liver disease (NAFLD) is the most common chronic liver disease, with a global prevalence of 25%. Patients with NAFLD are more likely to suffer from advanced liver disease, cardiovascular disease, or type II diabetes. However, unfortunately, there is still a shortage of FDA-approved therapeutic agents for NAFLD. Lian-Mei-Yin (LMY) is a traditional Chinese medicine formula used for decades to treat liver disorders. It has recently been applied to type II diabetes which is closely related to insulin resistance. Given that NAFLD is another disease involved in insulin resistance, we hypothesize that LMY might be a promising formula for NAFLD therapy. Herein, we verify that the LMY formula effectively reduces hepatic steatosis in diet-induced zebrafish and NAFLD model mice in a time-and dose-dependent manner. Mechanistically, LMY suppresses Yap1-mediated Foxm1 activation, which is crucial for the occurrence and development of NAFLD. Consequently, lipogenesis is ameliorated by LMY administration. In summary, the LMY formula alleviates diet-induced NAFLD in zebrafish and mice by inhibiting Yap1/Foxm1 signaling-mediated NAFLD pathology.

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“…Peroxisome proliferator-activated receptor γ coactivator-1α (PGC-1α) is the downstream effectors of AMPK which contributes to the regulation of biogenesis and function of mitochondria [32] . According to a previous study, crocetin could effectively restore the mitochondrial function [33] . It is notable that CLN significantly augmented the PGC-1α expression on fatigue mice, while elevated exhaustive swimming time compared with MC group.…”

Section: Cln Activates the Ampk/pgc-1α Signaling Pathway Of Fatigue Micementioning

confidence: 99%

Anti-fatigue activity and mechanism of crocetin loaded nanoliposome in acute exercise-treated mice

Nan,

Peng,

et al. 2024

Food Science and Human Wellness

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Crocetin displays strong antioxidant, anti-inflammatory and anti-depression activity which is promising to relieve symptoms of fatigue. But crocetin is difficult to dissolve in water and highly unstable against many environmental factors. Nanoliposome is used to encapsulate crocetin to improve its dispersion. The antifatigue activities and potential mechanism of crocetin loaded nanoliposome (CLN) was extensively investigated. The potential pathway of CLN on exerting antifatigue ability was investigated. Furthermore, impact of CLN on the gut microbiota structure was examined which contributes to its antifatigue functions. CLN significantly increases exhaustive swimming time of fatigue mice, decreases the blood contents of lactic, BUN and MDA. At the same time, CLN improves the activity of GSH-PX and SDH enzyme, attenuates the oxidant stress in mice. CLN activates the AMPK/PGC-1α signaling pathway of fatigue mice, increases the mRNA expression of ATP synthase. It also increases mRNA expression of mitochondrial transcription factor A (TFAM) which promotes mitochondrial biogenesis. Additionally, CLN ameliorates the gut microbiota structure by increasing the abundance of genus such as Lactobacillus in fatigue mice. In summary, CLN exerts strong anti-fatigue properties by decreasing the oxidant stress and the contents of harmful metabolites, augmenting the production of ATP, and potentially ameliorating the gut microbiota structure.

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Crocetin ameliorates non-alcoholic fatty liver disease by modulating mitochondrial dysfunction in L02 cells and zebrafish model

Cited by 12 publications

References 37 publications

An In Vitro Study of Saffron Carotenoids: The Effect of Crocin Extracts and Dimethylcrocetin on Cancer Cell Lines

An In Vitro Study of Saffron Carotenoids: The Effect of Crocin Extracts and Dimethylcrocetin on Cancer Cell Lines

Lian-Mei-Yin formula alleviates diet-induced hepatic steatosis by suppressing Yap1/FOXM1 pathway-dependent lipid synthesis

Anti-fatigue activity and mechanism of crocetin loaded nanoliposome in acute exercise-treated mice

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