2006
DOI: 10.1016/j.febslet.2006.08.038
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CRM1 mediates nuclear export of HDAC7 independently of HDAC7 phosphorylation and association with 14‐3‐3s

Abstract: CRM1, 14-3-3 proteins, and CaMK play important roles in trafficking of HDAC7, but the interplay between these proteins in this process is not clearly understood. Here, we show that CRM1 is capable of promoting cytoplasmic localization of wild-type and mutant HDAC7 (S178A/S344A/S479A), which is normally found in the nucleus. Using phospho-specific antibodies to HDAC7, we demonstrate that CaMK I promotes phosphorylation of S178, S344, and S479 of HDAC7. We also show that endogenous S178-phosphorylated HDAC7 is l… Show more

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Cited by 27 publications
(40 citation statements)
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“…Antibodies and Chemicals-␣-ACTN4 and ␣-HDAC7 antibodies have been previously described (5,16). Anti-HA-conjugated ␣-horseradish peroxidase was purchased from Roche Applied Science.…”
Section: Methodsmentioning
confidence: 99%
“…Antibodies and Chemicals-␣-ACTN4 and ␣-HDAC7 antibodies have been previously described (5,16). Anti-HA-conjugated ␣-horseradish peroxidase was purchased from Roche Applied Science.…”
Section: Methodsmentioning
confidence: 99%
“…Anti-HA-HRP antibody (12013819001) was from Roche. Purified HDAC7 and pS178-specific HDAC7 antibodies were previously described (20). The purified antibody did not cross-react with HDAC4 or HDAC5.…”
Section: Methodsmentioning
confidence: 99%
“…Expression plasmids for hemagglutinin (HA)-HDAC5 and -HDAC7, constitutively active CaMK I, green fluorescent protein (GFP)-HDAC7, glutathione S-transferase (GST)-HDAC7, HAubiquitin (Ub), FLAG-Ub, and the myocyte enhancer factor 2 (MEF2)-driven reporter construct have been previously described (19)(20)(21). HDAC7 constructs were generated from mouse cDNA.…”
Section: Methodsmentioning
confidence: 99%
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“…The HDAC7 residues critical for these processes are S181, S155, S358, and S486 (76). The latter three sites can be modified by CaMKI (77,78), whereas PKD can phosphorylate all four residues (76, 79 -83). In addition, hPar-1 kinases EMK and C-TAK1 phosphorylate S155 (84), and PRK1 targets S185 (68).…”
Section: Hdac5-although Numerous Hdac5mentioning
confidence: 99%