CRL4RBBP7 is required for efficient CENP-A deposition at centromeres

Mouysset, Julien; Gilberto, Samuel; Meier, Michelle G.; Lampert, Fabienne; Belwal, Mukta; Meraldi, Patrick; Peter, Matthias

doi:10.1242/jcs.162305

Cited by 23 publications

(19 citation statements)

References 76 publications

(104 reference statements)

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“…However, contradictory experimental evidence for the roles of CENP-A PTMs in this process means that the importance of the regulatory step remains somewhat murky. The ubiquitin ligase CUL4 in complex with RDX1-COPS8 or DDB1 has been shown to be important for CENP-A nucleosome deposition, without leading to CENP-A degradation [14,40,41]. Ubiquitylation of Lys124 of CENP-A is proposed to be required for CENP-A binding to HJURP.…”

Section: Pre-nucleosomal Posttranslational Modification Of Centromerimentioning

confidence: 99%

Posttranslational mechanisms controlling centromere function and assembly

Srivastava

Zasadzińska

Foltz

2018

Current Opinion in Cell Biology

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Accurate chromosome segregation is critical to ensure the faithful inheritance of the genome during cell division. Human chromosomes distinguish the location of the centromere from general chromatin by the selective assembly of CENP-A containing nucleosomes at the active centromere. The location of centromeres in most higher eukaryotes is determined epigenetically, independent of DNA sequence. CENP-A containing centromeric chromatin provides the foundation for assembly of the kinetochore that mediates chromosome attachment to the microtubule spindle and controls cell cycle progression in mitosis. Here we review recent work demonstrating the role of posttranslational modifications on centromere function and CENP-A inheritance via the direct modification of the CENP-A nucleosome and pre-nucleosomal complexes, the modification of the CENP-A deposition machinery and the modification of histones within existing centromeres.

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Section: Pre-nucleosomal Posttranslational Modification Of Centromerimentioning

confidence: 99%

Posttranslational mechanisms controlling centromere function and assembly

Srivastava

Zasadzińska

Foltz

2018

Current Opinion in Cell Biology

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“…19 Likewise, CRL4 RBBP7 was shown to aid timely deposition of the centromere-specific histone variant CENP-A during S-phase. 35 Furthermore, CRL4s at multiple steps coordinate timely cell cycle progression with DNA damage repair. Most prominently, CRL4…”

Section: Discussionmentioning

confidence: 99%

Guard the guardian: A CRL4 ligase stands watch over histone production

Lampert

Brodersen

Peter

2017

Nucleus

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Histones are evolutionarily conserved proteins that together with DNA constitute eukaryotic chromatin in a defined stoichiometry. Core histones are dynamic scaffolding proteins that undergo a myriad of post-translational modifications, which selectively engage chromosome condensation, replication, transcription and DNA damage repair. Cullin4-RING ubiquitin E3 ligases are known to hold pivotal roles in a wide spectrum of chromatin biology ranging from chromatin remodeling and transcriptional repression, to sensing of cytotoxic DNA lesions. Our recent work uncovers an unexpected function of a CRL4 ligase upstream of these processes in promoting histone biogenesis. The CRL4WDR23 ligase directly controls the activity of the stem-loop binding protein (SLBP), which orchestrates elemental steps of canonical histone transcript metabolism. We demonstrate that nonproteolytic ubiquitination of SLBP ensures sufficient histone reservoirs during DNA replication and is vital for genome integrity and cellular fitness.

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“…Four proteins (DDX39A, NUMA1, RBBP7, and DDX5) had a logFC greater than 0.5 in LECs and a unique peptide detection greater than 5 ( Figure 7b). Among these proteins, we identified the histone-binding protein RBBP7, which has previously been reported to be involved in the regulation of many cellular functions, including proliferation and migration 59,60 .…”

Section: Letr1 Interacts With Several Protein Complexes To Exert Its mentioning

confidence: 99%

LETR1 is a lymphatic endothelial-specific lncRNA that governs cell proliferation and migration through KLF4 and SEMA3C

Ducoli

Agrawal

Sibler

et al. 2020

Preprint

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Recent studies have revealed the importance of long noncoding RNAs (lncRNAs) as tissue-specific regulators of gene expression. There is ample evidence that distinct types of vasculature undergo tight transcriptional control to preserve their structure, identity, and functions. We determined, for the first time, the global lineage-specific lncRNAome of human dermal blood and lymphatic endothelial cells (BECs and LECs), combining RNA-Seq and CAGE-Seq. A subsequent genome-wide antisense oligonucleotide-knockdown screen of a robust set of BEC-and LEC-specific lncRNAs identified LESR2 as a critical gatekeeper of the global LEC transcriptome. Deep RNA-DNA, RNA-protein, and phenotype rescue analyses revealed that LESR2 acts as a nuclear trans-acting lncRNA modulating, via key epigenetic factors, the expression of essential target genes, including KLF4 and SEMA3C, governing the growth and migratory ability of LECs. Together, our study provides new evidence supporting the intriguing concept that every cell type expresses precise lncRNA signatures to control lineage-specific regulatory programs.The blood and lymphatic vascular systems are essential for the efficient transport of oxygen, nutrients, signaling molecules, and leukocytes to and from peripheral tissues, the removal of waste products, and the preservation of fluid homeostasis. Increased activation or impaired function of these vascular networks represent a hallmark of many pathological conditions, including cancer progression, chronic inflammatory diseases, and diseases leading to blindness 1-3 .During development, the blood vascular system arises from endothelial cell progenitors that differentiate from mesodermal cells, mostly through the expression of the transcription factor (TF) ETV2. Activation of the VEGFA/VEGFR2 signaling and expression of blood vascular endothelial cell (BEC) markers, such as NRP1 and EphrinB2, further differentiate these precursor cells into BECs, which then form the hierarchical network of blood vessels 4 . In contrast, lymphatic vasculogenesis starts after the establishment of the blood circulatory system. Thereafter, a distinct subpopulation of endothelial cells lining the cardinal vein starts differentiating by expressing the TF PROX1, the master regulator of lymphatic endothelial cell (LEC) identity, via the TFs SOX18 and COUPTFII. Once exiting the veins, LECs starts expressing other lymphatic-specific markers, such as podoplanin, VEGFR3, and NRP2, and they migrate, in a VEGFC-dependent manner, to form the primary lymph sacs from which the lymphatic vascular system further develops following sprouting, branching, proliferation, and remodeling processes 5 . However, a nonvenous origin of LECs has also been described in the skin, mesenteries, and heart 6-8 . In adulthood, while the blood and lymphatic vasculature are generally quiescent, they can be readily activated in pathological conditions such as wound healing, inflammation, and cancer by disturbance of the natural balance of pro-and anti-(lymph)angiogenic factors 1,9 . Therefor...

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CRL4RBBP7 is required for efficient CENP-A deposition at centromeres

Cited by 23 publications

References 76 publications

Posttranslational mechanisms controlling centromere function and assembly

Posttranslational mechanisms controlling centromere function and assembly

Guard the guardian: A CRL4 ligase stands watch over histone production

LETR1 is a lymphatic endothelial-specific lncRNA that governs cell proliferation and migration through KLF4 and SEMA3C

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