Critical size bone defect reconstruction by an autologous 3D osteogenic-like tissue derived from differentiated adipose MSCs

Schubert, Thomas; Lafont, Sébastien; Beaurin, Gwen; Grisay, Guillaume; Behets, Catherine; Gianello, Pierre; Dufrane, Denis

doi:10.1016/j.biomaterials.2013.02.053

Cited by 65 publications

(60 citation statements)

References 54 publications

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“…[34][35][36][37][38][39][40][41][42] Although the MSCs from bone marrow and adipose tissue are quite similar, there are some innate differences in their biology (bone marrow MSCs and ASCs can be differentiated with regard to functional gene expression) and release of growth factors in a hypoxic environment (as found in the bone nonunion tissue with <1% oxygen). 43,44 In a pig preclinical model, Schubert et al confirmed the superiority of ASCs in comparison to bone marrow MSCs. Indeed, they demonstrated the importance of osteogenic differentiation before implantation to promote better in vitro and in vivo angiogenesis (by VEGF release) and osteogenesis of the ASCs.…”

Section: Impact Of Age On Bone Marrow Msc Cellular Mechanism For Bonementioning

confidence: 63%

“…43 Schubert et al also investigated the potential of osteogenic ASCs with juvenile pig. [43][44][45] As with bone marrow-derived stem cells, questions remain about the impact of donor age in terms of ASC content, isolation, and efficacy (growth factor release by ASCs) to cure bone nonunion. Aside from financial considerations, the impact of age is the most important factor when determining if an autologous or allogeneic approach is better in clinical terms.…”

Section: Impact Of Age On Bone Marrow Msc Cellular Mechanism For Bonementioning

confidence: 99%

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Impact of age on human adipose stem cells for bone tissue engineering

Dufrane

2017

cell transplant

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Bone nonunion is a pathological condition in which all bone healing processes have stopped, resulting in abnormal mobility between 2 bone segments. The incidence of bone-related injuries will increase in an aging population, leading to such injuries reaching epidemic proportions. Tissue engineering and cell therapy using mesenchymal stem cells (MSCs) have raised the possibility of implanting living tissue for bone reconstruction. Bone marrow was first proposed as the source of stem cells for bone regeneration. However, as the quantity of MSCs in the bone marrow decreases, the capacity of osteogenic differentiation of bone marrow stem cells is also impaired by the donor's age in terms of reduced MSC replicative capacity; an increased number of apoptotic cells; formation of colonies positive for alkaline phosphatase; and decreases in the availability, growth potential, and temporal mobilization of MSCs for bone formation in case of fracture. Adipose-derived stem cells (ASCs) demonstrate several advantages over those from bone marrow, including a less invasive harvesting procedure, a higher number of stem cell progenitors from an equivalent amount of tissue harvested, increased proliferation and differentiation capacities, and better angiogenic and osteogenic properties in vivo. Subcutaneous native adipose tissue was not affected by the donor's age in terms of cellular senescence and yield of ASC isolation. In addition, a constant mRNA level of osteocalcin and alkaline phosphatase with a similar level of matrix mineralization of ASCs remained unaffected by donor age after osteogenic differentiation. The secretome of ASCs was also unaffected by age when aiming to promote angiogenesis by vascular endothelial growth factor (VEGF) release in hypoxic conditions. Therefore, the use of adipose cells for bone tissue engineering is not limited by the donor's age from the isolation of stem cells up to the manufacturing of a complex osteogenic graft.

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Section: Impact Of Age On Bone Marrow Msc Cellular Mechanism For Bonementioning

confidence: 63%

Section: Impact Of Age On Bone Marrow Msc Cellular Mechanism For Bonementioning

confidence: 99%

Impact of age on human adipose stem cells for bone tissue engineering

Dufrane

2017

cell transplant

Self Cite

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“…12,[16][17] Due to the biomimetic ECMs of a nanofiber scaffold, it might have significant effects on cell behavior. 2,10,18,19 In addition to the topographical features of a scaffold, the mechanical properties of a scaffold are also an important aspect, which could be regulated by the design of the structure and the material itself. A designed scaffold should not only provide a surface for cell prolif- eration but also maintain sufficient biomechanical support during tissue regeneration and structure degradation.…”

Section: Discussionmentioning

confidence: 99%

“…Briefly, PCEC copolymer was synthesized by introducing a known amount of ε-caprolactone and PEG (weight ratio =8:1) into a dry glass ampoule under N 2 atmosphere, with Sn(Oct) 2 (0.5% w/w of total feed stock) added as catalyst. During reaction, the system was stirred slowly, with the reaction temperature kept at 135°C.…”

Section: Materials and Methods Materialsmentioning

confidence: 99%

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Preparation and characterization of polylactide/poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) hybrid fibers for potential application in bone tissue engineering

Wang

Guo²,

Chen

et al. 2014

IJN

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The aim of this study was to develop a kind of osteogenic biodegradable composite graft consisting of human placenta-derived mesenchymal stem cell (hPMSC) material for site-specific repair of bone defects and attenuation of clinical symptoms. The novel nano- to micro-structured biodegradable hybrid fibers were prepared by electrospinning. The characteristics of the hybrid membranes were investigated by a range of methods, including Fourier transform infrared spectroscopy, X-ray diffraction, and differential scanning calorimetry. Morphological study with scanning electron microscopy showed that the average fiber diameter and the number of nanoscale pores on each individual fiber surface decreased with increasing concentration of poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) (PCEC). The prepared polylactide (PLA)/PCEC fibrous membranes favored hPMSC attachment and proliferation by providing an interconnected, porous, three-dimensional mimicked extracellular environment. What is more, hPMSCs cultured on the electrospun hybrid PLA/PCEC fibrous scaffolds could be effectively differentiated into bone-associated cells by positive alizarin red staining. Given the good cellular response and excellent osteogenic potential in vitro, the electrospun PLA/PCEC fibrous scaffolds could be one of the most promising candidates for bone tissue engineering.

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Iota‐carrageenan/chitosan/gelatin scaffold for the osteogenic differentiation of adipose‐derived MSCs in vitro

Yang

Qian

et al. 2014

J Biomed Mater Res

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In this study, we have developed ι-carrageenan/chitosan/gelatin (CCG) scaffold containing multiple functional groups (-NH2 , -OH, -COOH, and -SO3 H) to resemble the native extracellular matrix (ECM), using the ion-shielding technology and ultrasonic dispersion method. Fourier transform infrared spectroscopy (FTIR) of the CCG scaffolds suggests that the formation of CCG network involves electrostatic interactions between ι-carrageenan (ι-CA) and chitosan/gelatin, and the covalent cross-linking among amino groups of chitosan and/or gelatin. Scanning electron microscopic (SEM) observation reveals that the porous structure of scaffolds can be modulated by the ratio of ι-CA to chitosan/gelatin. The swelling ratio of the hydrogels increases as the ι-CA contents increase. Using differential scanning calorimetry, we found that the double helix structure of ι-CA is only stabilized at low contents of ι-CA in the CCG scaffolds (e.g., 5 wt %). The scaffolds containing 5% ι-CA showed the best protein adsorption capacity (4.46 ± 0.63 μg protein/mg scaffold) and elastic modulus (5.37 ± 1.03 MPa). In addition, the CCG scaffolds exhibit excellent support for adipose-derived mesenchymal stem cells (ADMSCs) attachment and proliferation, and they can improve the osteogenic differentiation and neovascularization capacities of ADMSCs. Overall, we conclude that the CCG may represent an ideal scaffold material for bone tissue engineering.

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Critical size bone defect reconstruction by an autologous 3D osteogenic-like tissue derived from differentiated adipose MSCs

Cited by 65 publications

References 54 publications

Impact of age on human adipose stem cells for bone tissue engineering

Impact of age on human adipose stem cells for bone tissue engineering

Preparation and characterization of polylactide/poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) hybrid fibers for potential application in bone tissue engineering

Iota‐carrageenan/chitosan/gelatin scaffold for the osteogenic differentiation of adipose‐derived MSCs in vitro

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Critical size bone defect reconstruction by an autologous 3D osteogenic-like tissue derived from differentiated adipose MSCs

Cited by 65 publications

References 54 publications

Impact of age on human adipose stem cells for bone tissue engineering

Impact of age on human adipose stem cells for bone tissue engineering

Preparation and characterization of polylactide/poly(&epsilon;-caprolactone)-poly(ethylene glycol)-poly(&epsilon;-caprolactone) hybrid fibers for potential application in bone tissue engineering

Iota‐carrageenan/chitosan/gelatin scaffold for the osteogenic differentiation of adipose‐derived MSCs in vitro

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Preparation and characterization of polylactide/poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) hybrid fibers for potential application in bone tissue engineering