Biochemical Pharmacology volume 81, issue 10, P1255-1261 2011 DOI: 10.1016/j.bcp.2011.03.004 View full text
Liesbeth Ceelen, Ward De Spiegelaere, Michael David, Jurgen De Craene, Mathieu Vinken, Tamara Vanhaecke, Vera Rogiers

Abstract: AbstractThe human HepaRG cell line has shown to be a valuable in vitro tool for repeated exposure to chemical compounds and to evaluate their potential toxic outcome. Seen the importance given by the actual EU legislation of cosmetics and chemical substances to the use of in vitro methods in human safety evaluation, one can expect that HepaRG cells will gain importance as human-relevant cell source. At the transcriptional level, RT-qPCR assays are often used to obtain quantitative results. The choice of inter…

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