Critical role of zinc in hardening of <i>Nereis</i> jaws

Broomell, Chris C.; Mattoni, Mike A.; Zok, Frank W.; Waite, J. Herbert

doi:10.1242/jeb.02373

Cited by 121 publications

(130 citation statements)

References 19 publications

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“…Alignment of sequences from all clones revealed an additional variant similar to NVjp1a (hereafter termed "NVjp-1a 1 and NVjp-1a 2 "), which consisted of the deletion of two GH repeats (residues [40][41][42][43] and an insertion of one additional GYGGHG repeat (residues 83-88, based on the sequence of NVjp-1a 1 ). The combined frequencies of NVjp-1a 1 , NVjp-1b, and NVjp-1a 2 from all cDNA preparations were 0.56, 0.33, and 0.11, respectively. The predicted amino acid compositions of all variants were nearly identical to each other (not shown) and to that of purified Nvjp-1 (Table 1, predicted cDNA sequence represents NVjp-1a 1 ).…”

Section: Resultsmentioning

confidence: 89%

“…The combined frequencies of NVjp-1a 1 , NVjp-1b, and NVjp-1a 2 from all cDNA preparations were 0.56, 0.33, and 0.11, respectively. The predicted amino acid compositions of all variants were nearly identical to each other (not shown) and to that of purified Nvjp-1 (Table 1, predicted cDNA sequence represents NVjp-1a 1 ). The range of predicted molecular weights of all three variants spans from ∼32 to ∼40 kDa.…”

Section: Resultsmentioning

confidence: 89%

“…1,3 The jaws are the worm's primary tools for feeding and defense and, as such, must be optimized for grasping, piercing, and tearing prey in an abrasive environment. Hardness and stiffness properties in the jaw are comparable to human dentin and superior to synthetic polymers.…”

Section: Introductionmentioning

confidence: 99%

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Cutting Edge Structural Protein from the Jaws of Nereis virens

et al. 2008

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The fang-like jaws of the marine polychaete Nereis Virens possess remarkable mechanical properties considering their high protein content and lack of mineralization. Hardness and stiffness properties in the jaw tip are comparable to human dentin and are achieved by extensive coordination of Zn 2+ by a histidine-rich protein framework. In the present study, the predominant protein in the jaw tip, NVjp-1, was purified and characterized by partial peptide mapping and molecular cloning of a partial cDNA from a jaw pulp library. The deduced amino acid sequence revealed an ∼38 kDa histidine-rich protein rich in glycine and histidine (∼36 and 27%, respectively) with no well-defined repetitive motifs. The effects of pH and metal treatment on aggregation, secondary structure, and hydrodynamic properties of recombinant Nvjp-1 are described. Notably, Zn treatment induced the formation of amyloid-like fibers.

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Section: Resultsmentioning

confidence: 89%

Section: Resultsmentioning

confidence: 89%

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Cutting Edge Structural Protein from the Jaws of Nereis virens

et al. 2008

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“…[60] Both Ala and Gly-rich motifs also exhibit resemblance to peptide sequences from the wing cuticles of migratory locusts, [64] oyster shell matrix proteins (Pinctada genus) [65] and chicken claw keratins. [66] Finally, His-rich SRT motifs exhibit similarity to His-rich proteins [67] of the King ragworm jaw (Nereis virens), another hard tissues made of sclerotized proteins that are mechanically reinforced via His-Zn coordination bonds [68][69][70] and dense covalent cross-linking. [71,72] Generally speaking, our combinatorial assay showed that Ala-rich and His-rich peptides exhibit the strongest homo-peptidic interactions and such interactions are pH-dependent, being weaker under acidic conditions and the strongest near neutral pH.…”

Section: Discussionmentioning

confidence: 99%

Modular peptides from the thermoplastic squid sucker ring teeth form amyloid-like cross-β supramolecular networks

et al. 2016

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. (2016). Modular peptides from the thermoplastic squid sucker ring teeth form amyloid-like cross-supramolecular networks. Acta Biomaterialia. https://doi.org/10.1016/j.actbio.2016.09.040Citing this paper Please note that where the full-text provided on King's Research Portal is the Author Accepted Manuscript or Post-Print version this may differ from the final Published version. If citing, it is advised that you check and use the publisher's definitive version for pagination, volume/issue, and date of publication details. And where the final published version is provided on the Research Portal, if citing you are again advised to check the publisher's website for any subsequent corrections. General rightsCopyright and moral rights for the publications made accessible in the Research Portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognize and abide by the legal requirements associated with these rights.•Users may download and print one copy of any publication from the Research Portal for the purpose of private study or research.•You may not further distribute the material or use it for any profit-making activity or commercial gain •You may freely distribute the URL identifying the publication in the Research Portal Take down policy If you believe that this document breaches copyright please contact librarypure@kcl.ac.uk providing details, and we will remove access to the work immediately and investigate your claim. This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. AbstractThe hard sucker ring teeth (SRT) from decapodiforme cephalopods, which are located inside the sucker cups lining the arms and tentacles of these squid species, have recently emerged as a unique model structure for biomimetic structural biopolymers. SRT are entirely composed of modular, block co-polymer-like proteins that self-assemble into a large supramolecular network. In order to unveil the molecular principles behind the SRT's self-assembly and robustness, we describe a combinatorial screening assay that maps the molecular-scale interactions between the most abundant modular peptide blocks of suckerin proteins. By selecting prominent interaction hotspots from this assay, we identified four peptides that exhibited the strongest homo-peptidic interactions, and conducted further in-depth biophysical characterizations complemented by molecular dynamic (MD) simulations to investigate the nature of these interactions. Circular Dichroism (CD) revealed conformations that transitioned from semi-extended poly-proline II (PII) towards β-sheet structure. The peptides s...

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“…The role of Cu as well as other metal ions in mechanical properties of bioorganic materials has been speculated to involve formation of intermolecular coordination complexes, creating a highly cross-linked molecular network. Although this effect has been clearly demonstrated in the case of Zn ions in the jaws of Nereis worms (17), it has been less compelling in the Glycera jaws because of the presence of Cucontaining atacamite fibers in neighboring regions. The present study indicates that Cu ions do indeed play a significant role in the mechanical properties of Glycera jaws, elevating the hardness and stiffness to levels that are about twice those obtained in the very best engineering polymers.…”

Section: Discussionmentioning

confidence: 99%

A nonmineralized approach to abrasion-resistant biomaterials

Pontin¹,

Moses

Waite

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

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The tooth-like mouthparts of some animals consist of biomacromolecular scaffolds with few mineral components, making them intriguing paradigms of biostructural materials. In this study, the abrasion resistance of the jaws of one such animal, the bloodworm Glycera dibranchiata, has been evaluated by nanoindentation, nanoscratching, and wear testing. The hardest, stiffest, and most abrasion-resistant materials are found within a thin (<3 m) surface layer near the jaw tip and a thicker (10 -20 m) subsurface layer, both rich in unmineralized Cu. These results are consistent with the supposition that Cu ions are involved in the formation of intermolecular coordination complexes between proteins, creating a highly cross-linked molecular network. The intervening layer contains aligned atacamite [Cu2(OH)3Cl] fibers and exhibits hardness and stiffness (transverse to the alignment direction) that are only slightly higher than those of the bulk material but lower than those of the two Cu-rich layers. Furthermore, the atacamitecontaining layer is the least abrasion-resistant, by a factor of Ϸ3, even relative to the bulk material. These observations are broadly consistent with the behavior of engineering polymer composites with hard fiber or particulate reinforcements. The alignment of fibers parallel to the jaw surface, and the fiber proximity to the surface, are both suggestive of a natural adaptation to enhance bending stiffness and strength rather than to endow the surface regions with enhanced abrasion resistance. glycera jaw ͉ nanoindentation ͉ scratching ͉ wear

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Critical role of zinc in hardening of Nereis jaws

Cited by 121 publications

References 19 publications

Cutting Edge Structural Protein from the Jaws of Nereis virens

Cutting Edge Structural Protein from the Jaws of Nereis virens

Modular peptides from the thermoplastic squid sucker ring teeth form amyloid-like cross-β supramolecular networks

A nonmineralized approach to abrasion-resistant biomaterials

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