Critical role of SDF-1/CXCR4 signaling pathway in stem cell homing in the deafened rat cochlea after acoustic trauma

Peyvandi, Ali Asghar; Roozbahany, Navid Ahmady; Peyvandi, Hassan; Abbaszadeh, Hojjat-Allah; Majdinasab, Nastaran; Faridan, Mohammad; Niknazar, Somayeh

doi:10.4103/1673-5374.224382

Cited by 30 publications

(14 citation statements)

References 54 publications

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“…b Semiquantitative analysis of fluorescence signals. Signal at the fracture site region of interest [ 7 ] was normalized to the background signal found in a similar region of interest in the contralateral unfractured femur. ( n = 6 mice).…”

Section: Resultsmentioning

confidence: 99%

The optimal time to inject bone mesenchymal stem cells for fracture healing in a murine model

Wang

Gou

et al. 2018

Stem Cell Res Ther

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BackgroundBone marrow is an important source of stem cells, which can promote bone fracture healing.MethodsWe investigated the optimal time to inject bone marrow mesenchymal stem cells (BMSCs) in a C57 murine unilateral, transverse, femur fracture model. BMSCs transfected with red fluorescent protein (RFP-BMSCs) were injected via the tail vein on day 1, 7, or 14 post-fracture. AMD3100 (inhibitor of stromal cell-derived factor 1 [SDF-1]) was also injected before RFP-BMSCs in one group for comparison; a control group received saline injections. RFP-BMSC migration and fracture healing were evaluated by in vivo fluorescence assay. Micro-CT was performed and mechanical testing and histological analysis. Chemokine levels were evaluated by quantitative real-time PCR and western blotting.ResultsFollowing injection on day 7 post-fracture, RFP-BMSCs more frequently homed to the fracture site and remained for a longer duration. Bone volume and bone mineral density were increased when BMSCs were injected on day 7 post-fracture (P < 0.05). The mechanical properties of fractured femurs were improved following day-7 BMSC injection. Histology confirmed that BMSC injection improved the formation of new bones.ConclusionsChemokines that induce BMSC migration were highly expressed, and protein levels of osteogenesis-related factors were increased. Seven days after fracture may be the optimal time for injection of BMSCs to promote fracture healing. Additionally, the SDF-1/CXCR4 pathway may play an important role in fracture healing following BMSC injection.

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Section: Resultsmentioning

confidence: 99%

The optimal time to inject bone mesenchymal stem cells for fracture healing in a murine model

Wang

Gou

et al. 2018

Stem Cell Res Ther

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“…MSCs show self-renewal and can differentiate into multiple cell lineages [ 27 , 28 ]. In addition to these properties, homing, in which cells migrate to injured areas owing to chemical gradients which has been suggested as a therapeutic advantage, is a characteristic of MSCs [ 6 , 29 ]. Nevertheless, stem cell therapy still has several disadvantages, such as poor isolation of the cells, efficiencies of migration and survival of the transplanted cells, and obscure mode of action [ 30 ].…”

Section: Discussionmentioning

confidence: 99%

“…Mesenchymal stem cell (MSC) therapy is a promising strategy for hepatic diseases through overexpression of the hepatic growth factor (HGF) and reduction of collagen and matrix metalloproteinase (MMP) [ 6 ]. Additionally, the effect of umbilical cord-MSCs (UC-MSCs) on the attenuation of fibrosis via the TGFb-1 receptor through the TGFb-1/SMAD pathway was elucidated [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

Exosomes from Placenta-Derived Mesenchymal Stem Cells Are Involved in Liver Regeneration in Hepatic Failure Induced by Bile Duct Ligation

Jun

Kim

Choi

et al. 2020

Stem Cells International

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Although the liver has a regenerative capacity, hepatic failure is a severe and irreversible chronic disease. Placenta-derived mesenchymal stem cells (PD-MSCs) have distinctive features, such as recycling of the placenta waste after birth, ease of accessibility, abundant cell numbers, and strong immunosuppressive properties. Previously, we reported that PD-MSCs can regenerate the liver in hepatic failure through antifibrotic and autophagic mechanisms. Many reports have investigated whether exosomes, which are formed by the budding of vesicular bodies and are emitted into the blood, from stem cells have therapeutic potential in various diseases. C-reactive protein (CRP) is produced in hepatocytes and secreted via vessels. Therefore, the objectives of this study were to compare the expression of CRP in exosomes of a hepatic failure rat model (bile duct ligation, BDL) and to evaluate the therapeutic effect by their correlation between CRP and angiogenesis depending on PD-MSC transplantation. The exosomes were analyzed in a BDL rat model with transplantation of PD-MSCs through LC-MS analysis and precipitation solution. The exosomes, CRP, and factors related to these molecules were evaluated and quantified in exosomes as well as investigated by real-time PCR, Western blot, and immunofluorescence (IF) in vivo and in vitro. CRP was present in exosomes from serum of a rat model and increased by PD-MSC transplantation. In the exosomes, CRP upregulated the factors related to the Wnt signaling pathway and angiogenesis in the BDL rat liver-transplanted PD-MSCs. Also, CRP regulated the Wnt pathway and vascularization in rat hepatocytes by interacting with endothelial cells. Therefore, our findings indicate that CRP in exosomes excreted by PD-MSCs functions in angiogenesis via the Wnt signaling pathway.

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“…Cochlear hair cell regeneration could be caused by direct interaction with hUCB cells, as well as by local or systemic paracrine effects caused by hUCB infusion. The cochlea is known to have resident macrophages, and macrophages can be recruited to the cochlea from circulating monocytes to damaged and dying inner hair cells [ 21 , 22 ]. A subset of cochlear support cells have been characterized as “stem-like” progenitor cells [ 23 ].…”

Section: Discussionmentioning

confidence: 99%

Safety of Autologous Umbilical Cord Blood Therapy for Acquired Sensorineural Hearing Loss in Children

et al. 2018

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Background and ObjectivesSensorineural hearing loss (SNHL) in children is associated with neurocognitive morbidity. The cause of SNHL is a loss of hair cells in the organ of Corti. There are currently no reparative treatments for SNHL. Numerous studies suggest that cord blood mononuclear cells (human umbilical cord blood, hUCB) allow at least partial restoration of SNHL by enabling repair of a damaged organ of Corti. Our objective is to determine if hUCB is a safe treatment for moderate to severe acquired SNHL in children.Subjects and MethodsEleven children aged 6 months to 6 years with moderate to severe acquired SNHL were treated with intravenous autologous hUCB. The cell dose ranged from 8 to 30 million cells/kg body weight. Safety was assessed by measuring systemic hemodynamics during hUCB infusion. Infusion-related toxicity was evaluated by measuring neurologic, hepatic, renal and pulmonary function before and after infusion. Auditory function, auditory verbal language assessments and MRI with diffusion tensor imaging (DTI) were obtained before and after treatment.ResultsAll patients survived, and there were no adverse events. No infusionrelated changes in hemodynamics occurred. No infusion-related toxicity was recorded. Five subjects experienced a reduction in auditory brainstem response (ABR) thresholds. Four of those 5 subjects also experienced an improvement in cochlear nerve latencies. Comparison of MRI with DTI sequences obtained before and after treatment revealed increased fractional anisotropy in the primary auditory cortex in three of five subjects with reduced ABR thresholds. Statistically significant (p<0.05) reductions in ABR thresholds were identified.ConclusionsTIntravenous hUCB is feasible and safe in children with SNHL.

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Critical role of SDF-1/CXCR4 signaling pathway in stem cell homing in the deafened rat cochlea after acoustic trauma

Cited by 30 publications

References 54 publications

The optimal time to inject bone mesenchymal stem cells for fracture healing in a murine model

The optimal time to inject bone mesenchymal stem cells for fracture healing in a murine model

Exosomes from Placenta-Derived Mesenchymal Stem Cells Are Involved in Liver Regeneration in Hepatic Failure Induced by Bile Duct Ligation

Safety of Autologous Umbilical Cord Blood Therapy for Acquired Sensorineural Hearing Loss in Children

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