Critical role of eukaryotic elongation factor 1 alpha 1 (EEF1A1) in avian reovirus sigma-C-induced apoptosis and inhibition of viral growth

Zhang, Zhiqiang; Lin, Wencheng; Li, Xiaoqi; Cao, Hong; Wang, Yongqiang; Zheng, Shijun

doi:10.1007/s00705-015-2403-5

Cited by 18 publications

(14 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Four of these ten conservative amino acid mutations were located in the C-terminal fragment, which might contribute to differences among the ARV strains in their receptor-binding ability. In addition, ARV σC is known as an apoptosis inducer via interaction with eukaryotic elongation factor 1α1 (EEF1A1) 19 20 21 22 , and a domain including residues 210–246 of σC is involved in interactions with EEF1A1 19 . The conservative amino acid mutation at residue 221 might influence the interaction of σC with EEF1A1 and/or the induction of apoptosis by σC.…”

Section: Discussionmentioning

confidence: 99%

Genetic and pathogenic characterisation of 11 avian reovirus isolates from northern China suggests continued evolution of virulence

Zhong

Gao

Liu

et al. 2016

Sci Rep

View full text Add to dashboard Cite

Avian reovirus (ARV) infections characterised by severe arthritis, tenosynovitis, pericarditis, and depressed growth have become increasingly frequent in recent years. In this study, we isolated and identified 11 ARV field strains from chickens with viral arthritis and reduced growth in northern China. Comparative analysis of the σC nucleotide and amino acid sequences demonstrated that all isolates, except LN05 and JS01, were closely related to ARV S1133 and clustered in the first genetic lineage. LN05 and JS01 strains were clustered in the third lineage with the ARV 138 strain. Using S1133 as a reference, five isolates were selected to infect specific-pathogen-free chickens, and we found that the recent isolated Chinese ARV strains had higher replication ability in vivo and caused enhanced mortality than the S1133 strain. These findings suggest that the pathogenicity of Chinese ARVs has been changing in recent years and disease control may become more difficult. This study provides genetic and pathogenic characterisations of ARV strains isolated in northern China and calls for a sustained surveillance of ARV infection in China in order to support a better prevention and control of the disease.

show abstract

Section: Discussionmentioning

confidence: 99%

Genetic and pathogenic characterisation of 11 avian reovirus isolates from northern China suggests continued evolution of virulence

Zhong

Gao

Liu

et al. 2016

Sci Rep

View full text Add to dashboard Cite

show abstract

“…One of these is EEF1A1 (Elongation factor 1-alpha 1), which is involved in tRNA delivery to the ribosome, and is known to be activated upregulated upon inflammation [52]. This protein has been shown to physically interact to proteins of several viral species, specifically hepatitis delta [53] and avian reoviruses [54]; in the latter, knock-down of EEF1A1 has been shown to significantly impair the virus infection cycle. Therefore, the downregulation of EEF1A1 could be the result of an innate cell strategy to deprive SARS-CoV-2 of a key support for RNA replication.…”

Section: Human/covid-19 Interactome Response To Coronavirus Infectionmentioning

confidence: 99%

Master Regulator Analysis of the SARS-CoV-2/Human interactome

Guzzi

Mercatelli

Ceraolo

et al. 2020

Preprint

103

119

View full text Add to dashboard Cite

the recent epidemic outbreak of a novel human coronavirus called SARS-CoV-2 and causing the respiratory tract disease COVID-19 has reached worldwide resonance and a global effort is being undertaken to characterize the molecular features and evolutionary origins of this virus. In this paper, we set out to shed light on the SARS-CoV-2/host receptor recognition, a crucial factor for successful virus infection. Based on the current knowledge of the interactome between SARS-CoV-2 and host cell proteins, we performed Master Regulator Analysis to detect which parts of the human interactome are most affected by the infection. We detected, amongst others, affected apoptotic and mitochondrial mechanisms, and a downregulation of the ACE2 protein receptor, notions that can be used to develop specific therapies against this new virus.

show abstract

“…Recently, studies have shown that in addition to the role in protein translation, the two sister genes, eEF1A1 and eEF1A2, exhibit some non-canonical functions. eEF1A1 has been extensively studied [ 8 – 11 ], but eEF1A2 has not. eEF1A2, unlike eEF1A1 which is widely expressed, is mainly expressed in the brain, heart and skeletal muscle [ 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

MLIF Alleviates SH-SY5Y Neuroblastoma Injury Induced by Oxygen-Glucose Deprivation by Targeting Eukaryotic Translation Elongation Factor 1A2

et al. 2016

View full text Add to dashboard Cite

Monocyte locomotion inhibitory factor (MLIF), a heat-stable pentapeptide, has been shown to exert potent anti-inflammatory effects in ischemic brain injury. In this study, we investigated the neuroprotective action of MLIF against oxygen-glucose deprivation (OGD)-induced injury in human neuroblastoma SH-SY5Y cells. MTT assay was used to assess cell viability, and flow cytometry assay and Hoechst staining were used to evaluate apoptosis. LDH assay was used to exam necrosis. The release of inflammatory cytokines was detected by ELISA. Levels of the apoptosis associated proteins were measured by western blot analysis. To identify the protein target of MLIF, pull-down assay and mass spectrometry were performed. We observed that MLIF enhanced cell survival and inhibited apoptosis and necrosis by inhibiting p-JNK, p53, c-caspase9 and c-caspase3 expression. In the microglia, OGD-induced secretion of inflammatory cytokines was markedly reduced in the presence of MLIF. Furthermore, we found that eukaryotic translation elongation factor 1A2 (eEF1A2) is a downstream target of MLIF. Knockdown eEF1A2 using short interfering RNA (siRNA) almost completely abrogated the anti-apoptotic effect of MLIF in SH-SY5Y cells subjected to OGD, with an associated decrease in cell survival and an increase in expression of p-JNK and p53. These results indicate that MLIF ameliorates OGD-induced SH-SY5Y neuroblastoma injury by inhibiting the p-JNK/p53 apoptotic signaling pathway via eEF1A2. Our findings suggest that eEF1A2 may be a new therapeutic target for ischemic brain injury.

show abstract

Critical role of eukaryotic elongation factor 1 alpha 1 (EEF1A1) in avian reovirus sigma-C-induced apoptosis and inhibition of viral growth

Cited by 18 publications

References 41 publications

Genetic and pathogenic characterisation of 11 avian reovirus isolates from northern China suggests continued evolution of virulence

Genetic and pathogenic characterisation of 11 avian reovirus isolates from northern China suggests continued evolution of virulence

Master Regulator Analysis of the SARS-CoV-2/Human interactome

MLIF Alleviates SH-SY5Y Neuroblastoma Injury Induced by Oxygen-Glucose Deprivation by Targeting Eukaryotic Translation Elongation Factor 1A2

Contact Info

Product

Resources

About