Critical review of single-cell mechanotyping approaches for biomedical applications

Chapman, Max; Rajagopal, Vijay; Stewart, Alastair; Collins, David J.

doi:10.1039/d3lc00978e

Lab Chip

2024

DOI: 10.1039/d3lc00978e

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Critical review of single-cell mechanotyping approaches for biomedical applications

Max Chapman,

Vijay Rajagopal,

Alastair Stewart

et al.

Abstract: Current approaches for mechanical measurements of single cells compromise between fidelity and throughput. Development of non-contact technologies and optimized theoretical modelling will advance mechanical characterisation of large cell populations.

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A Miniature Modular Fluorescence Flow Cytometry System

Huang,

Li,

Wei

et al. 2024

Biosensors

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Fluorescence flow cytometry is a powerful instrument to distinguish cells or particles labelled with high-specificity fluorophores. However, traditional flow cytometry is complex, bulky, and inconvenient for users to adjust fluorescence channels. In this paper, we present a modular fluorescence flow cytometry (M-FCM) system in which fluorescence channels can be flexibly arranged. Modules for particle focusing and fluorescence detection were developed. After hydrodynamical focusing, the cells were measured in the detection modules, which were integrated with in situ illumination and fluorescence detection. The signal-to-noise ratio of the detection reached to 33.2 dB. The crosstalk among the fluorescence channels was eliminated. The M-FCM system was applied to evaluate cell viability in drug screening, agreeing well with the commercial cytometry. The modular cytometry presents several outstanding features: flexibility in setting fluorescence channels, cost efficiency, compact construction, ease of operation, and the potential to upgrade for multifunctional measurements. The modular cytometry provides a multifunctional platform for various biophysical measurements, e.g., electrical impedance and refractive-index detection. The proposed work paves an innovative avenue for the multivariate analysis of cellular characteristics.

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A Miniature Modular Fluorescence Flow Cytometry System

Huang,

Li,

Wei

et al. 2024

Biosensors

View full text Add to dashboard Cite

show abstract

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Critical review of single-cell mechanotyping approaches for biomedical applications

Abstract: Current approaches for mechanical measurements of single cells compromise between fidelity and throughput. Development of non-contact technologies and optimized theoretical modelling will advance mechanical characterisation of large cell populations.

Cited by 1 publication

References 211 publications

A Miniature Modular Fluorescence Flow Cytometry System

A Miniature Modular Fluorescence Flow Cytometry System

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