Critical Field Appraisal for the Use of Bovine Tuberculosis’ Antibody Detecting-Serodiagnostics

Soliman, Rafik; Waheeb, Neven; Nasr, Eman; Elhariri, Mahmoud; Abo-Elyazeed, Heidy; Aboul-Ella, Hassan

doi:10.17582/journal.aavs/2023/11.2.278.287

Cited by 2 publications

(1 citation statement)

References 27 publications

(29 reference statements)

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“…This classification, whether depending on concentration detection or on OD value interpretation, is not strict and with testing more samples wider range may be figured out. The current research emphasizes the necessity for tests that assess humoral immune response in addition to the reference standardized tuberculin skin test, such as the lateral flow immunochromatographic (LFIC) strips or ELISA (Alonso et al 2021;Soliman et al 2023). The newly developed detection method created in this study may help bTB management programs and strategies be improved by reducing the number of positive Mycobacterium bovis-infected cattle that remain on dairy farms as well as continuously shed and spread the disease.…”

Section: Discussionmentioning

confidence: 99%

Development, Preparation and Evaluation of a Murine Monoclonal Antibodies Based Interferon-Gamma Enzyme-Linked Immunosorbent Assay (ELISA) for Rapid and Accurate Diagnosis of Bovine Tuberculosis

2023

Int J Vet Sci

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Section: Discussionmentioning

confidence: 99%

Development, Preparation and Evaluation of a Murine Monoclonal Antibodies Based Interferon-Gamma Enzyme-Linked Immunosorbent Assay (ELISA) for Rapid and Accurate Diagnosis of Bovine Tuberculosis

2023

Int J Vet Sci

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Development of novel Streptococcus equi vaccines with an assessment of their immunizing potentials and protective efficacies

Soliman,

Yousef,

gelil

et al. 2024

BMC Vet Res

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Strangles is a highly contagious disease of the equine upper respiratory tract caused by Streptococcus equi subspecies. Streptococcus equi subsp. equi (S. equi) and Streptococcus equi subsp. zooepidemicus (S. zooepidemicus) was isolated, as local, hot, and field strains, from horses clinically suffering from respiratory distress. The isolated Streptococci were identified using bacteriological and molecular techniques. Four formulations of inactivated S. equi vaccines were developed and evaluated. The first formulation was prepared using the S. equi isolates, adjuvanted with MONTANIDE GEL adjuvant, while the second formulation was adjuvanted with MONTANIDE ISA-70 adjuvant. The other 2 formulations were inactivated combined vaccines prepared from both S. equi and S. zooepidemicus isolates. The 3rd formulation was the combined isolates adjuvanted with MONTANIDE GEL while the 4th formulation was the combined isolates adjuvanted with MONTANIDE ISA-70. The developed vaccines’ physical properties, purity, sterility, safety, and potency were ensured. The immunizing efficacy was determined in isogenic BALB/c mice and white New Zealand rabbits using the passive hemagglutination test. Also, the antibodies’ titer of the combined S. equi and S. zooepidemicus vaccine adjuvanted with MONTANIDE ISA-70 in foals was tracked using an indirect enzyme-linked immunosorbent assay. The protective efficacy of the developed vaccines was determined using a challenge test in both laboratory and field animal models, where a 75% protection rate was achieved. The combined vaccine proved to be more efficacious than the monovalent vaccine. Also, the MONTANIDE ISA-70 adjuvant provided significant protective efficacy than the MONTANIDE GEL. The current work is introducing a very promising mitigative and strategic controlling solution for strangles.

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Critical Field Appraisal for the Use of Bovine Tuberculosis’ Antibody Detecting-Serodiagnostics

Abstract: The intradermal tuberculin skin test has been in use for almost a century in the diagnosis of bovine tuberculosis

Cited by 2 publications

References 27 publications

Development, Preparation and Evaluation of a Murine Monoclonal Antibodies Based Interferon-Gamma Enzyme-Linked Immunosorbent Assay (ELISA) for Rapid and Accurate Diagnosis of Bovine Tuberculosis

Development, Preparation and Evaluation of a Murine Monoclonal Antibodies Based Interferon-Gamma Enzyme-Linked Immunosorbent Assay (ELISA) for Rapid and Accurate Diagnosis of Bovine Tuberculosis

Development of novel Streptococcus equi vaccines with an assessment of their immunizing potentials and protective efficacies

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