Critical Factors Governing the Competitive Behaviour of Lactic Acid Bacteria in Mixed Cultures

Vandevoorde, L.; Woestyne, M. Vande; Bruyneel, B.; Christiaens, H.; Verstraete, Willy

doi:10.1007/978-1-4615-3522-5_18

Cited by 10 publications

(5 citation statements)

References 92 publications

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“…It is part of their cytochromes and of various iron‐sulfur proteins which are involved in energy metabolism . In contrast to most clostridia, LAB are devoid of cytochromes . Moreover, enzymes of the Embden‐Meyerhof‐Parnas (EMP) pathway used by LAB do not need iron as a cofactor.…”

Section: Discussionmentioning

confidence: 99%

Carboxylic acid production from ensiled crops in anaerobic solid‐state fermentation ‐ trace elements as pH controlling agents support microbial chain elongation with lactic acid

Sträuber

Bühligen

Kleinsteuber

et al. 2018

Engineering in Life Sciences

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Background: For the production of carboxylic acid platform chemicals like medium‐chain fatty acids (MCFA) by anaerobic fermentation, pH control is required. However, adding buffer solutions is ineffective in leach‐bed reactors. Aim: In order to increase the MCFA production by maize silage fermentation and to engineer the process we investigated the effect of solid alkaline iron and manganese additives on the process performance and microbial community dynamics. Results: Without additives, the pH dropped to 3.9 and lactic acid bacteria were favored. Total product yields of 207 ± 5.4 g organic acids (C2‐C6) and alcohols per kg volatile solids were reached. The addition of trace elements increased the pH value and the product spectrum and yields changed. With a commercial iron additive, the product yields were higher (293 ± 15.2 g/kgvolatile solids) and supposedly clostridia used lactic acid for microbial chain elongation of acetic acid producing n‐butyric acid. With the addition of pure Fe(OH)3 or Mn(OH)2, the total product yields were lower than in the other reactors. However, increased production of MCFA and the occurrence of distinct bacterial taxa (Lachnospiraceae, Ruminococcaceae and Megasphaera) related to this metabolic function were observed.Conclusions: The application of alkaline trace metal additives as pH stabilizing agents can mitigate spatial metabolic heterogeneities when trace metal deficient substrates like specific crops or residues thereof are applied.

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Section: Discussionmentioning

confidence: 99%

Carboxylic acid production from ensiled crops in anaerobic solid‐state fermentation ‐ trace elements as pH controlling agents support microbial chain elongation with lactic acid

Sträuber

Bühligen

Kleinsteuber

et al. 2018

Engineering in Life Sciences

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“…The exact way in which these antagonistic bioregulators (probiotics) act is not fully understood. They may produce compounds such as lactic acid, which are inhibitory to the growth of other pathogens (Havenaar et al., 1992; Vandevoorde et al., 1992), or they may compete with other pathogens for nutrients (Freter, 1992; Raibaud, 1992), or for attachment sites (Pollmann et al., 1980; Pedersen and Tannock, 1989; Fuller, 1992; Johnson and Conway, 1992; Stewart et al., 1993). It was also suggested that piglets receiving probiotics appear to have more advanced systemic and local immunity, so these bioregulators may exert some role by improving immunological mechanisms (Lessard and Brisson, 1987; Perdigon et al., 1990; Rodriguez‐Ropon et al., 1998).…”

Section: Discussionmentioning

confidence: 99%

Field Evaluation of a Bioregulator Containing Live Bacillus cereus Spores on Health Status and Performance of Sows and their Litters

Alexopoulos

Karagiannidis

Kritas

et al. 2001

Journal of Veterinary Medicine Series A

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The efficacy of Paciflor, a bioregulator containing live Bacillus cereus CIP 5832 spores, was assessed in sows during late pregnancy and lactation, as well as in their piglets up to the growing phase. Two groups each of 30 pregnant gilts and sows received normal feed (T1 group), or feed with 85 g Paciflor per ton feed (T2 group), from 15 days prior to farrowing up to the end of the lactation period. Furthermore, 15 litters of the T1 group and 15 litters of the T2 group, were offered normal feed from the 5th to the 70th days of life (T1.1 and T2.1 groups, respectively), while the remaining 15 litters each of the T1 and T2 groups received the same feed but including Paciflor at a dose of 100 g/ton (from day 5 to day 49) and 50 g/ton (from day 50 to day 70). These pig litters were T1.2 and T2.2, respectively. No differences were seen between the T1 and T2 groups with respect to the clinical observations (loss of appetite, fever, mastitis, metritis and returns to oestrus, treatments applied, deaths, or removals to the slaughterhouse), gestation length, bodyweight of sows at farrowing or litter-size at birth. However, during lactation, the fat content of the dam's milk was increased (0.46% more fat), the body weight loss of sows was reduced and the number of weaned pigs per sow was increased (0.6 more pigs per litter) after administration of Paciflor (P < 0.05). Weaning to service interval was also reduced by 1 day (P < 0.05). Moreover, piglets receiving Paciflor with their feed (T1.2 and T2.2 groups) showed less incidence of scours and lower mortality compared to the untreated piglets (T1.1 and T2.1 groups), particularly those pigs originating from Paciflor-treated dams (T2.2 group) (P < 0.05). Despite the fact that no difference was seen between groups with regard to the amount of feed consumed, the feed conversion ratio of Paciflor-treated piglets (T2.2 and T1.2) was significantly improved compared to that of the untreated piglets (T2.1 and T1.1) (P < 0.05). With respect to weight gain, for the Paciflor-treated piglets, those born to Paciflor-treated mothers (T2.2) were 0.56 kg heavier than those born to untreated dams (T1.2) (P < 0.05). It is concluded that administration of Paciflor in dams during the end of pregnancy and during lactation, as well as to their offspring during suckling and the flat-deck period is beneficial for the survival and growth of the piglets.

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“…Adherent stains may mask pathogen and toxin receptors without necessarily binding to the same epitope, and thus limit the ability of a pathogen to colonize and infect. Lactic acid bacteria are usually able to adhere to mucosal surfaces, a process mediated by carbohydrate, protein or lipoteichoic acid polymers depending on the specific strain studied (Vandervoorde et al 1992). Such bacteria form the basis of most probiotic preparations.…”

Section: Introductionmentioning

confidence: 99%

The effect of Lactobacillus spp. on the attachment of enterotoxigenic Escherichia coli to isolated porcine enterocytes

Spencer¹,

Chesson²

1994

Journal of Applied Bacteriology

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A total of 43 strains of lactobacilli were isolated from the gastrointestinal tract of piglets at the time of weaning. Isolates, grown on solid media, were allocated to strongly adherent or non/weakly adherent groups on the basis of numbers attaching to isolated porcine enterocytes. Strains of Lactobacillus fermentum were disproportionally represented amongst the strongly-adherent strains and Lact. acidophilus and Lact. salivarius amongst the non/weakly-adherent group. Lactobacilli showed significantly better attachment ability when grown on agar than when grown in broth culture. Strongly adherent strains were not found to effect the attachment of enterotoxigenic Escherichia coli to porcine enterocytes, tested under the conditions of exclusion (lactobacilli added to the enterocytes before E. coli), competition (lactobacilli and E. coli added simultaneously) and displacement (E. coli added before lactobacilli). Tests were made with [14C]-labelled E. coli. Suspensions of bacteria and enterocytes were passed through a filter selected to retain enterocytes but pass free bacterial cells. Counts (dpm) obtained from filters after solubilization were taken as a measure of E. coli attachment. Some strains of lactobacilli coaggregated with enterotoxigenic E. coli with K88 fimbriae, but not with a K88-negative mutants strain. These were excluded from the competitive exclusion experiments. In the apparent absence of a direct effect on the association of E. coli with host tissue, removal of potential gut pathogens by aggregation could contribute to the probiotic properties ascribed to lactic acid bacteria.

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Critical Factors Governing the Competitive Behaviour of Lactic Acid Bacteria in Mixed Cultures

Cited by 10 publications

References 92 publications

Carboxylic acid production from ensiled crops in anaerobic solid‐state fermentation ‐ trace elements as pH controlling agents support microbial chain elongation with lactic acid

Carboxylic acid production from ensiled crops in anaerobic solid‐state fermentation ‐ trace elements as pH controlling agents support microbial chain elongation with lactic acid

Field Evaluation of a Bioregulator Containing Live Bacillus cereus Spores on Health Status and Performance of Sows and their Litters

The effect of Lactobacillus spp. on the attachment of enterotoxigenic Escherichia coli to isolated porcine enterocytes

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