Critical evaluation and statistical validation of a hydroponic culture system for Arabidopsis thaliana

Smeets, Karen; Ruytinx, Joske; Belleghem, Frank Van; Semane, Brahim; Lin, Dan; Vangronsveld, Jaco; Cuypers, Ann

doi:10.1016/j.plaphy.2007.09.014

Cited by 65 publications

(34 citation statements)

References 28 publications

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“…Columbia seedlings were grown on hydroponics for 3 weeks (12 h photoperiod) (Smeets et al, 2008b). At that stage Cd (5 or 10 M) and Cu (2 or 5 M) were supplied as sulfates to the roots.…”

Section: Methodsmentioning

confidence: 99%

The cellular redox state as a modulator in cadmium and copper responses in Arabidopsis thaliana seedlings

Cuypers

Smeets

Ruytinx

et al. 2011

Journal of Plant Physiology

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295

176

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“…Columbia seedlings were grown on hydroponics for 3 weeks (12 h photoperiod) (Smeets et al, 2008b). At that stage Cd (5 or 10 M) and Cu (2 or 5 M) were supplied as sulfates to the roots.…”

Section: Methodsmentioning

confidence: 99%

The cellular redox state as a modulator in cadmium and copper responses in Arabidopsis thaliana seedlings

Cuypers

Smeets

Ruytinx

et al. 2011

Journal of Plant Physiology

Self Cite

295

176

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“…Wildtype A. thaliana seeds (Columbia ecotype) were surfacesterilised and seedlings were grown on hydroponics as described by Smeets et al (2008a), except that purified sand was used instead of rock wool (Keunen et al, 2011b). A modified Hoagland nutrient solution was used (Smeets et al, 2008a) and growth conditions were set at a 12 h photoperiod, 65% relative humidity and day/night temperatures of 22 • C and 18 • C respectively.…”

Section: Plant Culture and Cadmium Exposurementioning

confidence: 99%

“…A modified Hoagland nutrient solution was used (Smeets et al, 2008a) and growth conditions were set at a 12 h photoperiod, 65% relative humidity and day/night temperatures of 22 • C and 18 • C respectively. Light was provided by Philips Green-Power LED modules.…”

Section: Plant Culture and Cadmium Exposurementioning

confidence: 99%

“…During sampling, root and leaf biological replicates were harvested from -depending on the required sample weight -one or more seedlings out of one pot at a given point in time. To avoid within-pot correlation (Smeets et al, 2008a), different biological replicates were sampled out of other pots containing the same Cd concentration.…”

Section: Plant Culture and Cadmium Exposurementioning

confidence: 99%

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Alternative respiration as a primary defence during cadmium-induced mitochondrial oxidative challenge in Arabidopsis thaliana

Keunen

Jozefczak

Remans

et al. 2013

Environmental and Experimental Botany

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a b s t r a c tPlant growth and development can be highly restricted by environmental stressors such as cadmium (Cd) pollution. The mitochondrial non-phosphorylating alternative respiratory pathway, mediated by alternative oxidase (AOX), alternative NAD(P)H dehydrogenases (NDs) and uncoupling protein (UCP), was suggested to be crucial in the acclimation of plants to fluctuating environmental conditions. Therefore, we examined the effects of environmentally realistic Cd exposure (5 and 10 M) on the alternative respiratory chain in Arabidopsis thaliana using a kinetic exposure setup. We demonstrated that during exposure to Cd, Arabidopsis seedlings show a mitochondrial oxidative challenge to which they acutely respond by increasing the transcript level of several AOX, ND and UCP isoforms in both roots and leaves. In addition, AOX protein levels increased during acute Cd exposure (2 and 24 h). Based on our data, we suggest the formation of a condensed non-phosphorylating electron transport chain (ETC) functioning through cytosolic NDs and AOX, with co-regulation of ND and AOX expression during Cd stress. Therefore, both enzymes might cooperate in the potential acclimation of Arabidopsis seedlings to environmentally realistic Cd exposure by modulating the extent of mitochondrial ROS production.

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“…The sterilised seeds were germinated on agar plates containing Murashige and Skoog (1962) salts and 1% (w/v) agar. After 1 week of germination, the seedlings were transferred to hydroponic growing facilities described by Smeets et al (2008) with day and night temperatures of 20 and 24°C, respectively. The light intensity was supplied by 4-foot Xuorescent tubes (Philip daylight tube, 36 W) in a 16 h light cycle.…”

Section: Plant Materials and Growth Conditionmentioning

confidence: 99%

The PAL2 promoter activities in relation to structural development and adaptation in Arabidopsis thaliana

Wong

Namasivayam

Abdullah

2011

Planta

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Phenylalanine ammonia lyase (PAL) plays a major role in plant growth, development and adaptation. In Arabidopsis thaliana, the enzyme is encoded by four genes, namely PAL1, PAL2, PAL3, and PAL4 with PAL1 and PAL2 being closely related phylogenetically and functionally. PAL1 promoter activities are associated with plant development and are inducible by various stress agents. However, PAL2 promoter activities have not been functionally analysed. Here, we show that the PAL2 promoter activities are associated with the structural development of a plant and its organs. This function was inducible in an organ-specific manner by the avirulent strain of Pseudomonas syringae pv. tomato (JL1065). The PAL2 promoter was active throughout the course of the plant development particularly in the root, rosette leaf, and inflorescence stem that provide the plant with structural support. In aerial organs, the levels of PAL2 promoter activities were negatively correlated with relative positions of the organs to the rosette leaves. The promoter was inducible in the root following an inoculation by JL1065 in the leaf suggesting PAL2 to be part of an induced defence system. Our results demonstrate how the PAL2 promoter activities are being coordinated and synchronised for the structural development of the plant and its organs based on the developmental programme. Under certain stress conditions the activity may be induced in favour of certain organs.

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Critical evaluation and statistical validation of a hydroponic culture system for Arabidopsis thaliana

Cited by 65 publications

References 28 publications

The cellular redox state as a modulator in cadmium and copper responses in Arabidopsis thaliana seedlings

The cellular redox state as a modulator in cadmium and copper responses in Arabidopsis thaliana seedlings

Alternative respiration as a primary defence during cadmium-induced mitochondrial oxidative challenge in Arabidopsis thaliana

The PAL2 promoter activities in relation to structural development and adaptation in Arabidopsis thaliana

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