Critical Comparison of Liquid Chromatography Coupled to Mass Spectrometry and Three Different Ion Mobility Spectrometry Systems on Their Separation Capability for Small Isomeric Compounds

Werres, Tobias; Leonhardt, Juri; Jäger, Martin; Teutenberg, Thorsten

doi:10.1007/s10337-018-3640-z

Cited by 12 publications

(8 citation statements)

References 18 publications

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“…This example shows that the sole use of additives in the drift gas is likely not sufficient to separate isomers. This finding is supported by a comparative study in which directly infused steroid hormones, using different ion mobility instruments and SR (DMA with acetonitrile and DMS with 2-propanol), showed no difference in resolution with the use of additives ( Werres et al, 2019 ).…”

Section: Ims-ms Approaches Towards the Separation Of Geometrical Lipi...mentioning

confidence: 70%

Integrating the potential of ion mobility spectrometry-mass spectrometry in the separation and structural characterisation of lipid isomers

Camunas‐Alberca

Morán-Garrido

Sáiz

et al. 2023

Front. Mol. Biosci.

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It is increasingly evident that a more detailed molecular structure analysis of isomeric lipids is critical to better understand their roles in biological processes. The occurrence of isomeric interference complicates conventional tandem mass spectrometry (MS/MS)-based determination, necessitating the development of more specialised methodologies to separate lipid isomers. The present review examines and discusses recent lipidomic studies based on ion mobility spectrometry combined with mass spectrometry (IMS-MS). Selected examples of the separation and elucidation of structural and stereoisomers of lipids are described based on their ion mobility behaviour. These include fatty acyls, glycerolipids, glycerophospholipids, sphingolipids, and sterol lipids. Recent approaches for specific applications to improve isomeric lipid structural information using direct infusion, coupling imaging, or liquid chromatographic separation workflows prior to IMS-MS are also discussed, including: 1) strategies to improve ion mobility shifts; 2) advanced tandem MS methods based on activation of lipid ions with electrons or photons, or gas-phase ion-molecule reactions; and 3) the use of chemical derivatisation techniques for lipid characterisation.

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Section: Ims-ms Approaches Towards the Separation Of Geometrical Lipi...mentioning

confidence: 70%

Integrating the potential of ion mobility spectrometry-mass spectrometry in the separation and structural characterisation of lipid isomers

Camunas‐Alberca

Morán-Garrido

Sáiz

et al. 2023

Front. Mol. Biosci.

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“…As already demonstrated for the combination of LC and IM‐MS, the CE separation through the measurement of the effective mobility values ( μ eff ) appeared to be more powerful than the IM separation [31]. For isomeric analytes and due to the strong correlation of CCS with m/z , most of the isomers could not be distinguished by their CCS (relative CCS difference <2%).…”

Section: Resultsmentioning

confidence: 99%

Evaluation of ion mobility in capillary electrophoresis coupled to mass spectrometry for the identification in metabolomics

et al. 2020

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Currently, feature annotation remains one of the main challenges in untargeted metabolomics. In this context, the information provided by high‐resolution mass spectrometry (HRMS) in addition to accurate mass can improve the quality of metabolite annotation, and MS/MS fragmentation patterns are widely used. Accurate mass and a separation index, such as retention time or effective mobility (μeff), in chromatographic and electrophoretic approaches, respectively, must be used for unequivocal metabolite identification. The possibility of measuring collision cross‐section (CCS) values by using ion mobility (IM) is becoming increasingly popular in metabolomic studies thanks to the new generation of IM mass spectrometers. Based on their similar separation mechanisms involving electric field and the size of the compounds, the complementarity of DTCCSN2 and μeff needs to be evaluated. In this study, a comparison of DTCCSN2 and μeff was achieved in the context of feature identification ability in untargeted metabolomics by capillary zone electrophoresis (CZE) coupled with HRMS. This study confirms the high correlation of DTCCSN2 with the mass of the studied metabolites as well as the orthogonality between accurate mass and μeff, making this combination particularly interesting for the identification of several endogenous metabolites. The use of IM‐MS remains of great interest for facilitating the annotation of neutral metabolites present in the electroosmotic flow (EOF) that are poorly or not separated by CZE.

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Section: Im‐ms To Separate Metabolites and Lipidsmentioning

confidence: 99%

“…The separation of isomers usually requires high IMS resolving power due to the minimal structural differences that are often associated with isomeric ions in gas phase (Causon & Hann, 2015;Kaufmann et al, 2020;Werres et al, 2019) (Table 1). Hofmann et al (2015) reported the IMS separation of six trisaccharide isomers that differed in composition, connectivity, or configuration.…”

Section: Isomers Separationmentioning

confidence: 99%

Ion mobility mass spectrometry in the omics era: Challenges and opportunities for metabolomics and lipidomics

Paglia

Smith

Astarita

2021

Mass Spectrometry Reviews

106

100

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Researchers worldwide are taking advantage of novel, commercially available, technologies, such as ion mobility mass spectrometry (IM‐MS), for metabolomics and lipidomics applications in a variety of fields including life, biomedical, and food sciences. IM‐MS provides three main technical advantages over traditional LC‐MS workflows. Firstly, in addition to mass, IM‐MS allows collision cross‐section values to be measured for metabolites and lipids, a physicochemical identifier related to the chemical shape of an analyte that increases the confidence of identification. Second, IM‐MS increases peak capacity and the signal‐to‐noise, improving fingerprinting as well as quantification, and better defining the spatial localization of metabolites and lipids in biological and food samples. Third, IM‐MS can be coupled with various fragmentation modes, adding new tools to improve structural characterization and molecular annotation. Here, we review the state‐of‐the‐art in IM‐MS technologies and approaches utilized to support metabolomics and lipidomics applications and we assess the challenges and opportunities in this growing field.

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Critical Comparison of Liquid Chromatography Coupled to Mass Spectrometry and Three Different Ion Mobility Spectrometry Systems on Their Separation Capability for Small Isomeric Compounds

Cited by 12 publications

References 18 publications

Integrating the potential of ion mobility spectrometry-mass spectrometry in the separation and structural characterisation of lipid isomers

Integrating the potential of ion mobility spectrometry-mass spectrometry in the separation and structural characterisation of lipid isomers

Evaluation of ion mobility in capillary electrophoresis coupled to mass spectrometry for the identification in metabolomics

Ion mobility mass spectrometry in the omics era: Challenges and opportunities for metabolomics and lipidomics

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