Critical assessment of recent trends related to screening and confirmatory analytical methods for selected food contaminants and allergens

Tsagkaris, Aristeidis S.; Nelis, Joost L.D.; Ross, G.M.S.; Jafari, S.; Guercetti, Julian; Kopper, Klaudia; Zhao, Yunfeng; Rafferty, Karen; Salvador, J.‐Pablo; Migliorelli, Davide; Salentijn, Gert Ij; Campbell, Katrina; Marco, Marı́a Pilar; Elliot, Christopher; Nielen, Michel W. F.; Pulkrabová, Jana; Hajšlová, Jana

doi:10.1016/j.trac.2019.115688

Cited by 70 publications

(61 citation statements)

References 97 publications

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“…Two recent review papers have extensively outlined commercially-available and proof-of-concept single-plex and multiplex allergen immunoassays and biosensors, and the assays reported in this study have matched or surpassed these previously-reported LODs [9,51]. All the developed multiplex assays were able to detect both analytes in the low ppm range within minutes.…”

Section: Discussionsupporting

confidence: 60%

“…Additionally, the PHC assay was validated as a screening method in spiked matrix extract, blank matrix extract (n = 20) and incurred spiked flour, proving its capability of detecting the target even in complex matrices. The majority of commercially-available allergen detection LFIA test kits can detect a single analyte at 1-10 ppm [9,51]. Comparatively, PHC was able to detect both analytes at 0.5 ppm of THP and TPP spiked into a blank biscuit matrix extract, affirming its place as one of the most sensitive allergen LFIAs.…”

Section: Discussionmentioning

confidence: 79%

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A Critical Comparison between Flow-through and Lateral Flow Immunoassay Formats for Visual and Smartphone-Based Multiplex Allergen Detection

2019

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(1) Background: The lack of globally standardized allergen labeling legislation necessitates consumer-focused multiplexed testing devices. These should be easy to operate, fast, sensitive and robust. (2) Methods: Herein, we describe the development of three different formats for multiplexed food allergen detection, namely active and passive flow-through assays, and lateral flow immunoassays with different test line configurations. (3) Results: The fastest assay time was 1 min, whereas even the slowest assay was within 10 min. With the passive flow approach, the limits of detection (LOD) of 0.1 and 0.5 ppm for total hazelnut protein (THP) and total peanut protein (TPP) in spiked buffer were reached, or 1 and 5 ppm of THP and TPP spiked into matrix. In comparison, the active flow approach reached LODs of 0.05 ppm for both analytes in buffer and 0.5 and 1 ppm of THP and TPP spiked into matrix. The optimized LFIA configuration reached LODs of 0.1 and 0.5 ppm of THP and TPP spiked into buffer or 0.5 ppm for both analytes spiked into matrix. The optimized LFIA was validated by testing in 20 different blank and spiked matrices. Using device-independent color space for smartphone analysis, two different smartphone models were used for the analysis of optimized assays.

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Section: Discussionsupporting

confidence: 60%

Section: Discussionmentioning

confidence: 79%

A Critical Comparison between Flow-through and Lateral Flow Immunoassay Formats for Visual and Smartphone-Based Multiplex Allergen Detection

2019

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“…However, AChE activity is reduced in the presence of CMs or OPs, due to carbamylation or phosphorylation of serine hydroxyl group in the enzyme active cite [3], respectively. This results in acetylcholine accumulation, which can Liquid and/or gas chromatography tandem mass spectrometry (LC-MS/MS and/or GC-MS/MS) are commonly applied for pesticide residues monitoring in food matrices [8]. Although these methods provide selectivity, sensitivity with high-throughput capabilities, LC/MS and GC/MS are also costly and time-consuming methodologies that require skilled personnel [9].…”

Section: Introductionmentioning

confidence: 99%

“…During assay optimization, various parameters were investigated including a critical comparison of commonly applied AChE substrates for the first time. Following our recently published paper, which addressed the issues related to the validation and benchmarking of biosensors in food safety [8], comprehensive validation datasets for carbofuran, carbofuran-3-hydroxy and dichlorvos in strawberry and lettuce extracts are presented. The optimized AChE assay was benchmarked towards an accredited LC-MS/MS method, following the regulatory requirement (Decision 2002/657/EC) of confirming bioassays results with an instrumental method.…”

Section: Introductionmentioning

confidence: 99%

Screening of Carbamate and Organophosphate Pesticides in Food Matrices Using an Affordable and Simple Spectrophotometric Acetylcholinesterase Assay

et al. 2020

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Carbamates (CMs) and organophosphates (OPs) are widely used pesticides with known neurotoxicity arising from the inhibition of acetylcholinesterase (AChE). When AChE is active, in vitro, it can hydrolyze certain substrates to colored products while in the presence of an inhibitor this color development is decreased. Based on this principle, an AChE assay for CM and OP compounds was optimized and validated for carbofuran, carbofuran-3-hydroxy and dichlorvos in lettuce and strawberry extracts. The analytical performance of the assay was confirmed by an accredited liquid chromatography tandem mass spectrometry (LC–MS/MS) method. The developed AChE assay achieved low limits of detection (LODs) at the part per billion (ppb) level, depending the analyte inhibitory strength, recovery rates higher than 70% and good repeatability. Moreover, the toxic unit (TU) approach was applied, for extracts containing the validated analytes, and antagonism was noticed in all cases. Overall, the developed method is rapid, simple, cost-effective and may find application as a low-cost pre-screening tool of AChE inhibitors presence. Last but not least, this study can be considered a guide on development, validation and benchmarking of bioassays in food safety, a topic, which is commonly mispresented in the available literature.

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“…Malachite green and its metabolites are toxic, mutagenic, and carcinogenic. A number of studies have been described for the determination of antibiotics, malachite green, and leucomalachite green in different sample treatment methods and instrumental determination methods [15][16][17][18]. However, these methods are focused only on single-drug class of veterinary residues, which leads to low efficiency and high cost when different physicochemical properties of various veterinary drugs are determined separately.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Determination of Malachite Green, Chloramphenicols, Sulfonamides, and Fluoroquinolones Residues in Fish by Liquid Chromatography-Mass Spectrometry

Chen¹,

Shuping

Han³

et al. 2020

Journal of Analytical Methods in Chemistry

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A fast-analytical method using simplified extraction has been developed for the simultaneous determination of 42 compounds from 4 different classes of veterinary drugs (amphenicols, triphenylmethane, fluoroquinolones, and sulfonamides) in fish by reverse phase liquid chromatography-tandem mass spectrometry. The selection of extraction reagents was optimized using different types of microfiltration membrane, mobile phase, and LC column. Samples were extracted using 0.4% hydrochloric acid in acetonitrile and ethyl acetate and then were cleaned up using solid-phase extraction Cleanert Alumina N columns (500 mg) and Oasis hydrophilic-lipophilic balance (HLB) cartridges. The chromatographic separation was performed on a XR-ODS C8 column using a mobile phase of (A) 0.1% formic acid and 2 mM ammonium acetate and (B) 0.1% formic acid acetonitrile at a flow rate of 0.25 mL·min−1. The results indicated 67.7–112.8% recovery of 42 compounds with an intra- and interday relative standard deviations less than 10%. The limits of quantification for analytes were in the range of 0.3–1.0 μg kg−1 for samples which were satisfactory to support future surveillance monitoring. The method applicability was checked by analyzing 30 fish samples collected from local markets. Two fish samples surpassed the established MRL of 100 μg kg−1 with values of 104 μg kg−1 and 112 μg kg−1.

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Critical assessment of recent trends related to screening and confirmatory analytical methods for selected food contaminants and allergens

Cited by 70 publications

References 97 publications

A Critical Comparison between Flow-through and Lateral Flow Immunoassay Formats for Visual and Smartphone-Based Multiplex Allergen Detection

A Critical Comparison between Flow-through and Lateral Flow Immunoassay Formats for Visual and Smartphone-Based Multiplex Allergen Detection

Screening of Carbamate and Organophosphate Pesticides in Food Matrices Using an Affordable and Simple Spectrophotometric Acetylcholinesterase Assay

Simultaneous Determination of Malachite Green, Chloramphenicols, Sulfonamides, and Fluoroquinolones Residues in Fish by Liquid Chromatography-Mass Spectrometry

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