Critical aspects of fluorescent age-pigment methodologies: Modification for accurate analysis and age assessments in aquatic organisms

Hill, Kevin T.; Womersley, Christopher

doi:10.1007/bf01320225

Cited by 30 publications

(16 citation statements)

References 57 publications

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“…The fluorescence intensity of each sample was determined at an emission maximum of 450 nm for Aequipecten opercularis and 435 nm for Adamussium colbecki. Following Hill & Womersley (1991), lipofuscin concentrations were expressed as relative fluorescent intensity (RFI) using 0.1 µg quinine sulphate ml -1 1 N H 2 SO 4 as standard. Protein content.…”

Section: Sampling and Maintenance Aequipecten Opercularismentioning

confidence: 99%

Physiological ageing in a temperate and a polar swimming scallop

Philipp¹,

Brey²,

Heilmayer³

et al. 2006

Mar. Ecol. Prog. Ser.

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We compared physiological ageing parameters in 2 scallops, the temperate Aequipecten opercularis and the Antarctic Adamussium colbecki. These 2 species are phylogenetically closely related and display a similar lifestyle but have distinctly different maximum lifespans (MLSP). A. opercularis does not live longer than 8 to 10 yr, whereas A. colbecki lives over 18 yr. The development of several physiological ageing parameters over time, chosen according to the 'free radical theory of ageing', was compared in the 2 species to identify differences in the ageing process. In the shorter-lived A. opercularis, activities of the mitochondrial enzymes citrate synthase and cytochrome c oxidase and of the antioxidant enzyme catalase showed a more pronounced decrease with increasing age than in the longer-lived A. colbecki. In line with this finding, lipofuscin accumulation increased more distinctly in A. opercularis than in A. colbecki, while tissue protein content decreased in A. opercularis but increased in A. colbecki. Its better preservation of mitochondrial and antioxidant enzyme activities and the avoidance of waste accumulation may enable A. colbecki to live longer than A. opercularis. Mitochondrial function investigated in A. opercularis showed only minor changes with age, and mitochondrial H 2 O 2 generation rates were low at all ages. We relate our findings to the 'free radical-rate of living' theory, to the 'uncoupling to survive' hypothesis, and to the particular lifestyle of these scallops. KEY WORDS: Ageing · scallops · Reactive oxygen species · ROS · MitochondriaResale or republication not permitted without written consent of the publisher

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Section: Sampling and Maintenance Aequipecten Opercularismentioning

confidence: 99%

Physiological ageing in a temperate and a polar swimming scallop

Philipp¹,

Brey²,

Heilmayer³

et al. 2006

Mar. Ecol. Prog. Ser.

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“…The luminescence of the sample was determined at the emission maximum (Nicol, 1987) at 435 nm in case of S. officinalis. Lipofuscin concentrations are expressed as relative fluorescence intensity (RFI) according to Hill and Womersley (1991), using 0.1 mg quinine per ml 1 N H 2 SO 4 as a standard.…”

Section: Assay Of Lipofuscinmentioning

confidence: 99%

Oxidative stress and antioxidative defense in cephalopods: a function of metabolic rate or age?

Zielinski

Pörtner

2000

Comparative Biochemistry and Physiology Part B: Biochemistry an

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Activities of the antioxidative enzymes superoxide dismutase (SOD), catalase, glutathione peroxidase (GPX) and glutathione reductase (GR) were measured in the cephalopods Sepia officinalis and Lolliguncula bre6is. Maximal enzyme activities were higher in gill tissue than in the mantle musculature of both species. Activities were generally lower in tissues of L. bre6is than in S. officinalis. Comparison with other ectothermic animals showed both cephalopod species to have a low enzymatic antioxidative status despite their high metabolic rate. Furthermore, changes in antioxidative enzyme activities were measured in the cuttlefish S. officinalis with increasing age. The concentrations of malondialdehyde (MDA) and lipofuscin were determined as indicators of lipid peroxidation. Investigated animals were between 1.5 months and over 12 months old. Changes of antioxidative enzyme activities with age were not uniform. SOD and GPX activities increased with age, while catalase activity declined. In contrast, GR activity remained almost unchanged in all age groups. The low level of antioxidative defense might allow for the significant age-induced rise in MDA levels in gills and mantle musculature and for the increase in lipofuscin levels in mantle and brain tissue. It might thereby contribute to increased oxidative damage and a short life span in these cephalopods.

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“…The few studies relating lipofuscin to age in molluscs (Clarke et al, 1990;Zielinski and Pörtner, 2000;Sukhotin et al, 2002) are based on solvent extraction of autofluorescent substances. As there is increasing evidence that such autofluorescence is not related to lipofuscin in situ (Nicol, 1987;Hill and Womersley, 1991;Sheehy and Roberts, 1991;Sheehy 1996Sheehy , 2002Jolly et al, 2002;Palmer et al, 2002, Porta 2002Schmucker and Sachs, 2002), we will limit the discussion to studies based on lipofuscin in situ measured in situ in histological sections.…”

Section: Introductionmentioning

confidence: 99%

Survivorship of juvenile surf clams Donax serra (Bivalvia, Donacidae) exposed to severe hypoxia and hydrogen sulphide

Laudien

Schiedek

Brey

et al. 2002

Journal of Experimental Marine Biology and Ecology

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In the hard clam Eurhomalea exalbida, autofluorescent granules were detected in high concentrations in the connective tissue around the intestine and in other tissues. Autofluorescence combined with Sudan black B and PAS positive reactions suggested that these granules were lipofuscin-like. The concentration of this material in the connective tissue (CT) around the intestine was quantified by image analysis and expressed as total area fraction occupied by lipofuscin granules. Lipofuscin concentration was distinctly better related with individual age as determined from stable isotope-validated shell growth bands than with any morphometric parameter. This relationship was described best by a Gompertz model: Lipofuscin t = 24.79e À e À 0.029(Age t À 58.578) (N = 38; r nl 2 = 0.882). Age was predicted from lipofuscin CT concentration by a von Bertalanffy model: Age t = 68.00 (1 À e À 0.146(Lipofuscin t + 0.028) ) 0.664 (N = 38; r nl 2 = 0.933). Our findings suggest that lipofuscin CT concentration in E. exalbida is a function of individual age. If this holds true for bivalves in general, lipofuscin may be a suitable proxy for age in species with less clear shell growth band patterns. D

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Critical aspects of fluorescent age-pigment methodologies: Modification for accurate analysis and age assessments in aquatic organisms

Cited by 30 publications

References 57 publications

Physiological ageing in a temperate and a polar swimming scallop

Physiological ageing in a temperate and a polar swimming scallop

Oxidative stress and antioxidative defense in cephalopods: a function of metabolic rate or age?

Survivorship of juvenile surf clams Donax serra (Bivalvia, Donacidae) exposed to severe hypoxia and hydrogen sulphide

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