Critical Appraisal of Salting-Out and Its Implications for Chemical and Biological Sciences

Grover, Phulwinder K.; Ryall, Rosemary L.

doi:10.1021/cr030454p

Cited by 173 publications

(151 citation statements)

References 122 publications

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“…The temperature must be kept low to avoid denaturation of the proteins. Mechanistically, organic solvents reduce the proteins' solubility by decreasing the medium's dielectric constant (12). The stability of proteins' native structures in water-cosolvent mixtures has been examined in the context of enzyme catalysis in "good and bad solvents" (13).…”

Section: Protein Purificationmentioning

confidence: 99%

Affinity-Based Strategies for Protein Purification

Mondal

Gupta²,

Roy³

2006

Anal. Chem.

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Section: Protein Purificationmentioning

confidence: 99%

Affinity-Based Strategies for Protein Purification

Mondal

Gupta²,

Roy³

2006

Anal. Chem.

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“…When surfactant and salt are mixed in solution, salting-out phenomenon often happens [15][16][17][18]. According to hydration theory [19] salting-out is the result of preferential movement of water molecules, which immobilize and quench their role as solvents, from coordination shells of surfactant molecules to those of salts. The effects of halide salts on the growth of micelles in ionic surfactant solutions have been systematically studied [20][21].…”

Section: Resultsmentioning

confidence: 99%

Micellization Behavior of Cetyltrimethylammonium bromide in Presence and Absence of Na2SO4 and MgSO4 in Aqueous media

2016

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Viscosity and surface tension measurements of cetyltrimethylammonium bromide (CTAB) in presence and absence of Na 2 SO 4 and MgSO 4 in aqueous media are reported. The results showed an increase in viscosity with increase in concentration of cetyltrimethylammonium bromide in presence and absence of salts where as the decrease in surface tension with increase in concentration of cetyltrimethylammonium bromide in presence and absence of salts. The viscosity of CTAB was observed lowest in pure water and increases in presence of MgSO 4 and then more increases in presence of Na 2 SO 4 where as the surface tension of CTAB was observed highest in pure water and decreases in presence of Na 2 SO 4 and then more decreases in presence of MgSO 4 .The critical micelle concentration (cmc) of CTAB decreases in presence of salts in both viscosity and surface tension measurements. The decreasing cmc of CTAB are found to be more in the presence of Na 2 SO 4 in comparison with the presence of MgSO 4 for both measurements. The graphs of viscosity versus log[C] and the surface tension versus log[C] are used in determining the critical micelle concentration (cmc).

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“…According to hydration theory [10] salting-out is the result of preferential movement of water molecules, which immobilize and quench their role as solvents, from coordination shells of surfactant molecules to those of salts. The effects of halide salts on the growth of micelles in ionic surfactant solutions have been systematically studied [11,12].…”

Section: Resultsmentioning

confidence: 99%

Micellization behaviour of sodium dodecyl sulphate in presence and absence of sodium sulphate and zinc sulphate in distilled water by surface tension measurement

2014

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The accurate measurements of surface tension of sodium dodecyl sulphate (SDS) in distilled water and in presence of 0.01M Na 2 SO 4 and 0.01M ZnSO 4 at room temperature were reported by drop weight method using a stalagmometer. The critical micelle concentration (cmc) of sodium dodecyl sulphate (SDS) in distilled water was obtained higher than in presence of Na 2 SO 4 and ZnSO 4 . The decrease of cmc of sodium dodecyl sulphate in the presence of salts has been discussed.

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Critical Appraisal of Salting-Out and Its Implications for Chemical and Biological Sciences

Cited by 173 publications

References 122 publications

Affinity-Based Strategies for Protein Purification

Affinity-Based Strategies for Protein Purification

Micellization Behavior of Cetyltrimethylammonium bromide in Presence and Absence of Na2SO4 and MgSO4 in Aqueous media

Micellization behaviour of sodium dodecyl sulphate in presence and absence of sodium sulphate and zinc sulphate in distilled water by surface tension measurement

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