CRISPR Technology in Gene-Editing-Based Detection and Treatment of SARS-CoV-2

Abstract

Outbreak and rapid spread of coronavirus disease (COVID-19) caused by coronavirus acute respiratory syndrome (SARS-CoV-2) caused severe acute respiratory syndrome (SARS-CoV-2) that started in Wuhan, and has become a global problem because of the high rate of human-to-human transmission and severe respiratory infections. Because of high prevalence of SARS-CoV-2, which threatens many people worldwide, rapid diagnosis and simple treatment are needed. Genome editing is a nucleic acid-based approach to altering the genome by artificially changes in genetic information and induce irreversible changes in the function of target gene. Clustered, regularly interspaced short palindromic repeats (CRISPR/Cas) could be a practical and straightforward approach to this disease. CRISPR/Cas system contains Cas protein, which is controlled by a small RNA molecule to create a double-stranded DNA gap. Evidence suggested that CRISPR/Cas was also usable for diagnosis and treatment of SARS-CoV-2 infection. In this review study, we discoursed on application of CRISPR technology in detection and treatment of SARS-CoV-2 infection. Another aspect of this study was to introduce potential future problems in use of CRISPR/Cas technology.

Insights into Cardiovascular Defects and Cardiac Epigenome in the Context of COVID-19

“…In recent years, CRISPR (clustered regularly interspaced short palindromic repeats) has been successfully implemented in various applications including the editing of epigenetic factors [ 190 , 191 ] and as a potential option to address heart disease [ 192 ]. Based on the success of CRISPR, it is not surprising that CRISPR has been used to detect SARS-CoV-2 [ 193 , 194 ] and the advantages of CRISPR in various aspects of COVID-19 treatment are being considered [ 195 , 196 ]. As CRISPR is already established in the editing of epigenetic factors and cardiac research, it is only a matter of time before CRISPR is developed as a significant tool against COVID-19-related cardiac and epigenetic complications.…”

A Review of SARS-CoV-2 Disease (COVID-19): Pandemic in Our Time

“…However, we must bear in mind that the CRISPR technology has its own limitations too since it is pretty much new technology that is still being worked on to understand more of it. The advancement in the technological world has made almost all of these methods achievable within 24 h or even less [ 75 , 76 , 77 , 78 , 79 , 80 , 81 ].…”

COVID‐19 in early 2023: Structure, replication mechanism, variants of SARS‐CoV‐2, diagnostic tests, and vaccine & drug development studies

“… 57 It was reported that depending on the assay technique, CRISPR‐COVID assay costs less than $3.50 for a single reaction. 58 In the near future, CRISPR‐based assays have the potential to become less complicated, more reliable, faster, and more affordable. 59 The detection time is 2–8 h for ELISA.…”

“…SHERLOCK for SARS‐CoV‐2 detection has demonstrated 93.1% sensitivity and is significantly faster than qRT‐PCR 57 . It was reported that depending on the assay technique, CRISPR‐COVID assay costs less than $3.50 for a single reaction 58 . In the near future, CRISPR‐based assays have the potential to become less complicated, more reliable, faster, and more affordable 59 .…”