CRISPR technology for genome editing

Bhattacharjee, Gargi; Mani, Indra; Gohil, Nisarg; Khambhati, Khushal; Braddick, Darren; Panchasara, Happy; Singh, Vijai

doi:10.1016/b978-0-12-819178-1.00007-1

Cited by 9 publications

(4 citation statements)

References 89 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, when the intended gene editing has been performed, all traces of the gene-editing components must be eliminated ( Metje-Sprink et al., 2019 ; Miroshnichenko et al., 2019 ). Recent advancements in genome editing tools including DNA-free delivery methods and base editing systems solved this problem and provide a wide opportunity to edit plant genomes in a precise manner ( Bhattacharjee et al., 2020 ). Advanced tools such as direct delivery method, and delivery of in vitro assembled ribonucleoprotein (Cas9/gRNA) and use of virus-like particles and employment of bacterial secretory systems for Cas/gRNA delivery are the main approaches that have been employed to accomplish DNA-free genome editing.…”

Section: Advancements In Genome Editingmentioning

confidence: 99%

CRISPR-Cas: A robust technology for enhancing consumer-preferred commercial traits in crops

et al. 2023

View full text Add to dashboard Cite

The acceptance of new crop varieties by consumers is contingent on the presence of consumer-preferred traits, which include sensory attributes, nutritional value, industrial products and bioactive compounds production. Recent developments in genome editing technologies provide novel insight to identify gene functions and improve the various qualitative and quantitative traits of commercial importance in plants. Various conventional as well as advanced gene-mutagenesis techniques such as physical and chemical mutagenesis, CRISPR-Cas9, Cas12 and base editors are used for the trait improvement in crops. To meet consumer demand, breakthrough biotechnologies, especially CRISPR-Cas have received a fair share of scientific and industrial interest, particularly in plant genome editing. CRISPR-Cas is a versatile tool that can be used to knock out, replace and knock-in the desired gene fragments at targeted locations in the genome, resulting in heritable mutations of interest. This review highlights the existing literature and recent developments in CRISPR-Cas technologies (base editing, prime editing, multiplex gene editing, epigenome editing, gene delivery methods) for reliable and precise gene editing in plants. This review also discusses the potential of gene editing exhibited in crops for the improvement of consumer-demanded traits such as higher nutritional value, colour, texture, aroma/flavour, and production of industrial products such as biofuel, fibre, rubber and pharmaceuticals. In addition, the bottlenecks and challenges associated with gene editing system, such as off targeting, ploidy level and the ability to edit organelle genome have also been discussed.

show abstract

Section: Advancements In Genome Editingmentioning

confidence: 99%

CRISPR-Cas: A robust technology for enhancing consumer-preferred commercial traits in crops

et al. 2023

View full text Add to dashboard Cite

show abstract

“…Despite the invention of MNs, ZFNs, and TALENs fairly ahead of the discovery of CRISPR-Cas9, the aforementioned three molecular techniques remain significantly unutilized in the case of plants considering the requirement for complex protein engineering systems and high cost and time . So far CRISPR has shown some impressive results in achieving precise genetic modification at single or multiple gene targets. , The CRISPR-Cas9 system has been used to improve and incorporate several desirable traits in plants, of which betterment in disease resistance is one such trait. Besides their application in plants, CRISPR systems have also been used to target proteins that are known to play key roles in the interaction between fungal and oomycete pathogens and host plants .…”

Section: Recent Developments In Nucleic Acid Based Detectionmentioning

confidence: 99%

Emerging Extraction and Diagnostic Tools for Detection of Plant Pathogens: Recent Trends, Challenges, and Future Scope

Verma

Shukla

Kulkarni

et al. 2022

ACS Agric. Sci. Technol.

Self Cite

View full text Add to dashboard Cite

Plant pathogens are a serious threat to agriculture for long-term viability and cost loss of billions of dollars yearly. Many pathogens have been documented in the literature that infect a variety of crops. Nevertheless, new pathogens emerge and often result in disease outbreaks, leading to million-dollar losses. Currently, several diagnostic approaches with enhanced sensitivity and specificity for the identification of widespread and/or unknown plant pathogens are constantly being developed. Whereas the extensively used approaches for plant pathogen diagnostics are mostly serological and nucleic acid-based assays, many different nucleic acid-based approaches for amplifying target DNA/RNA have also emerged over time. However, these approaches lack precision, specificity, and rapidity, making them unsuitable for on-field analysis. As a result, there is a lot of interest arising in fielddeployable point of care (POC) devices and artificial intelligence (AI)-assisted pathogens' detection accurately at an early stage within a minute. Similarly, development of a cell-lysis and purification-free DNA/RNA extraction process is also crucial for quick sample preparation for molecular diagnosis of plant pathogens at field level. In this review, we have discussed advanced tools that are trending not only to extract nucleic acids but also detect plant pathogens. We have also discussed critical challenges and future perspectives of disease diagnostic tools for plant pathogens' detection. In summary, advanced plant disease diagnostic tools can be helpful for routine monitoring of plant pathogens toward improving crop productivity and yield that can be used for improving the financial status of farmers.

show abstract

“…36 Class 1 systems (types I, III, IV) are those with effector complexes using multiple Cas proteins, and Class 2 systems (types II, V, VI) are those that use a single Cas protein. [37][38][39] The CRISPR-Cas systems are based on the RNA-directed endonuclease mechanism that provides adaptive immunity to bacteria against the invading nucleic acids. 40 DNA sequences based on past encounters, called spacers, are stored in the host chromosome and are used by Cas proteins to detect and cleave invading nucleic acids in cells.…”

Section: Introductionmentioning

confidence: 99%

“…CRISPR‐Cas systems are divided into two classes and several subtypes 36 . Class 1 systems (types I, III, IV) are those with effector complexes using multiple Cas proteins, and Class 2 systems (types II, V, VI) are those that use a single Cas protein 37–39 . The CRISPR‐Cas systems are based on the RNA‐directed endonuclease mechanism that provides adaptive immunity to bacteria against the invading nucleic acids 40 .…”

Section: Introductionmentioning

confidence: 99%

Phage engineering and phage‐assisted CRISPR‐Cas delivery to combat multidrug‐resistant pathogens

Khambhati

Bhattacharjee

Gohil

et al. 2022

Bioengineering & Transla Med

Self Cite

View full text Add to dashboard Cite

Antibiotic resistance ranks among the top threats to humanity. Due to the frequent use of antibiotics, society is facing a high prevalence of multidrug resistant pathogens, which have managed to evolve mechanisms that help them evade the last line of therapeutics. An alternative to antibiotics could involve the use of bacteriophages (phages), which are the natural predators of bacterial cells. In earlier times, phages were implemented as therapeutic agents for a century but were mainly replaced with antibiotics, and considering the menace of antimicrobial resistance, it might again become of interest due to the increasing threat of antibiotic resistance among pathogens. The current understanding of phage biology and clustered regularly interspaced short palindromic repeats (CRISPR) assisted phage genome engineering techniques have facilitated to generate phage variants with unique therapeutic values. In this review, we briefly explain strategies to engineer bacteriophages. Next, we highlight the literature supporting CRISPR-Cas9-assisted phage engineering for effective and more specific targeting of bacterial pathogens. Lastly, we discuss techniques that either help to increase the fitness, specificity, or lytic ability of bacteriophages to control an infection.

show abstract

CRISPR technology for genome editing

Cited by 9 publications

References 89 publications

CRISPR-Cas: A robust technology for enhancing consumer-preferred commercial traits in crops

CRISPR-Cas: A robust technology for enhancing consumer-preferred commercial traits in crops

Emerging Extraction and Diagnostic Tools for Detection of Plant Pathogens: Recent Trends, Challenges, and Future Scope

Phage engineering and phage‐assisted CRISPR‐Cas delivery to combat multidrug‐resistant pathogens

Contact Info

Product

Resources

About