CRISPR Systems for COVID-19 Diagnosis

Rahimi, Hossein; Salehiabar, Marziyeh; Barsbay, Murat; Ghaffarlou, Mohammadreza; Kavetskyy, Taras; Sharafi, Ali; Davaran, Soodabeh; Chauhan, Subhash C.; Danafar, Hossein; Kaboli, Saeed; Nosrati, Hamed; Yallapu, Murali M.; Conde, João

doi:10.1021/acssensors.0c02312

Cited by 113 publications

(94 citation statements)

References 118 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Feng Zhang and his colleagues developed SHERLOCK system which uses Cas13 enzyme along with a programmed CRISPR system, which can target specific gene sequence for cleavage, degradation of target strand and activation of the fluorescent reporter [ 55 ]. In both systems when the guide RNA finds the specific sequence in the sample, it cleaves the identified sequence and gives fluorescence.…”

Section: Details On Covid-19mentioning

confidence: 99%

A comprehensive review on current COVID-19 detection methods: From lab care to point of care diagnosis

Singh¹,

Datta²,

Ashish³

et al. 2021

Sensors International

View full text Add to dashboard Cite

Without a doubt, the current global pandemic affects all walks of our life. It affected almost every age group all over the world with a disease named COVID-19, declared as a global pandemic by WHO in early 2020. Due to the high transmission and moderate mortality rate of this virus, it is also regarded as the panic-zone virus. This potentially deadly virus has pointed up the significance of COVID-19 research. Due to the rapid transmission of COVID-19, early detection is very crucial. Presently, there are different conventional techniques are available for coronavirus detection like CT-scan, PCR, Sequencing, CRISPR, ELISA, LFA, LAMP. The urgent need for rapid, accurate, and cost-effective detection and the requirement to cut off shortcomings of traditional detection methods, make scientists realize to advance new technologies. Biosensors are one of the reliable platforms for accurate, early diagnosis. In this article, we have pointed recent diagnosis approaches for COVID-19. The review includes basic virology of SARS-CoV-2 mainly clinical and pathological features. We have also briefly discussed different types of biosensors, their working principles, and current advancement for COVID-19 detection and prevention.

show abstract

Section: Details On Covid-19mentioning

confidence: 99%

A comprehensive review on current COVID-19 detection methods: From lab care to point of care diagnosis

Singh¹,

Datta²,

Ashish³

et al. 2021

Sensors International

View full text Add to dashboard Cite

show abstract

“…Clustered regularly interspaced short palindromic repeats (CRISPR)-based diagnostic systems were also proposed for COVID-19 diagnosis. CRISPR is a family of DNA sequences found in the genomes of prokaryotic organisms such as bacteria [209][210][211]. They are used to detect and destroy DNA similarly to bacteriophages during infections.…”

Section: Genetic-based Analysis: Pcr Rt-qpcr Lamp and Crisprmentioning

confidence: 99%

“…Lateral flow assay based on CRISPR-based diagnostic systems was reported [209]. The method is rapid (< 40 min), easy to implement, and showed high accuracy for the detection of SARS-CoV 2 from respiratory swab RNA extracts [213].…”

Section: Genetic-based Analysis: Pcr Rt-qpcr Lamp and Crisprmentioning

confidence: 99%

Nanobiotechnology as a platform for the diagnosis of COVID-19: a review

Abdelhamid

Badr

2021

Nanotechnol. Environ. Eng.

View full text Add to dashboard Cite

A sensitive method for diagnosing coronavirus disease 2019 (COVID-19) is highly required to fight the current and future global health threats due to severe acute respiratory syndrome coronavirus 2 (SARS-CoV 2). However, most of the current methods exhibited high false-negative rates, resulting in patient misdiagnosis and impeding early treatment. Nanoparticles show promising performance and great potential to serve as a platform for diagnosing viral infection in a short time and with high sensitivity. This review highlighted the potential of nanoparticles as platforms for the diagnosis of COVID-19. Nanoparticles such as gold nanoparticles, magnetic nanoparticles, and graphene (G) were applied to detect SARS-CoV 2. They have been used for molecular-based diagnosis methods and serological methods. Nanoparticles improved specificity and shorten the time required for the diagnosis. They may be implemented into small devices that facilitate the self-diagnosis at home or in places such as airports and shops. Nanoparticles-based methods can be used for the analysis of virus-contaminated samples from a patient, surface, and air. The advantages and challenges were discussed to introduce useful information for designing a sensitive, fast, and low-cost diagnostic method. This review aims to present a helpful survey for the lesson learned from handling this outbreak to prepare ourself for future pandemic.

show abstract

“…Rapid diagnostic methods of detecting SARS-CoV-2 selectively, either qualitatively or quantitively, are well-established and have proven to help control virus transmission (Kaushik et al, 2020;Ahmadivand et al, 2021;Morales-Narváezand Dincer, 2020). The gold standard currently used is real-time polymerase chain reaction (RT-PCR) to deliver accurate information but it is timeconsuming (Giovannini et al, 2021;Rahimi et al, 2021). In contrast, other methods such as the rapid antigen testing can be done quickly but can only detect a large count of COVID-19 antigens from the concentrations in the range of 1484 copies/mL to 780 × 10 6 copies/mL (Fenollar et al, 2021).…”

Section: Efficient Detection Of Newer Severe Acute Respiratory Syndrome Coronavirus 2 Variantsmentioning

confidence: 99%

“…However, crossvalidation has been suggested by health agencies. Besides, new types of biomarkers such as the CRISPR system (Rahimi et al, 2021) can be a choice to detect new SARS-CoV-2 selectively.…”

Section: Efficient Detection Of Newer Severe Acute Respiratory Syndrome Coronavirus 2 Variantsmentioning

confidence: 99%

Perspectives of Manipulative and High-Performance Nanosystems to Manage Consequences of Emerging New Severe Acute Respiratory Syndrome Coronavirus 2 Variants

Gage

Brunson

Morris

et al. 2021

Front. Nanotechnol.

View full text Add to dashboard Cite

The emergence of new SARS-CoV-2 variants made the COVID-19 infection pandemic and/or endemic more severe and life-threatening due to ease of transmission, rapid infection, high mortality, and capacity to neutralize the therapeutic ability of developed vaccines. These consequences raise questions on established COVID-19 infection management strategies based on nano-assisted approaches, including rapid diagnostics, therapeutics, and efficient trapping and virus eradication through stimuli-assisted masks and filters composed of nanosystems. Considering these concerns as motivation, this perspective article highlights the role and aspects of nano-enabled approaches to manage the consequences of the COVID-19 infection pandemic associated with newer SARS-CoV-2 variants of concern and significance generated due to mutations. The controlled high-performance of a nanosystem seems capable of effectively detecting new variables for rapid diagnostics, performing site-specific delivery of a therapeutic agent needed for effective treatment, and developing technologies to purify the air and sanitizing premises. The outcomes of this report project manipulative, multifunctional nanosystems for developing high-performance technologies needed to manage consequences of newer SARS-CoV-2 variants efficiently and effectively through an overall targeted, smart approach.

show abstract

CRISPR Systems for COVID-19 Diagnosis

Cited by 113 publications

References 118 publications

A comprehensive review on current COVID-19 detection methods: From lab care to point of care diagnosis

A comprehensive review on current COVID-19 detection methods: From lab care to point of care diagnosis

Nanobiotechnology as a platform for the diagnosis of COVID-19: a review

Perspectives of Manipulative and High-Performance Nanosystems to Manage Consequences of Emerging New Severe Acute Respiratory Syndrome Coronavirus 2 Variants

Contact Info

Product

Resources

About