CRISPR system in the yeast Saccharomyces cerevisiae and its application in the bioproduction of useful chemicals

Mitsui, Ryosuke; Yamada, R.; Ogino, Hiroyasu

doi:10.1007/s11274-019-2688-8

Cited by 25 publications

(14 citation statements)

References 68 publications

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“…These examples display yeast cells and synthetic biology strategies as an inspirational–but above all powerful–duo. Indeed, microorganisms are highly suitable for the generation of heterologous producers; this is due to their ease of manipulation, fast growth, and the availability of versatile molecular tools [5] . Nevertheless, metabolic engineering remains challenging and commonly requires, besides multiplex genomic integration, the fine‐tuning of gene expression, and the redesign of endogenous primary metabolism.…”

Section: Figurementioning

confidence: 99%

Microbial Cell Factories for Tetrahydroisoquinoline Alkaloid Production

2020

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For decades, plants have represented an inexhaustible source of natural products used in various sectors such as health and industry. However, one recurring problem is the low accumulation of these compounds in planta and, therefore, their production costs and supply. In recent years, unprecedented hope has been brought by the metabolic engineering of microorganisms, which opens up prospects for supply of these molecules at lower cost with high added value. However, many of these productions remained at a laboratory scale. In a recent article published in Nature Communication, Vincent J. J. Martin's team has developed an optimized yeast strain capable of synthesizing not only a huge amount of (S)‐reticuline, a major precursor of the plant tetrahydroisoquinoline alkaloid series, but also a whole range of new‐to‐nature compounds from this prominent family of natural products. This synthesis, reaching industrial scales, thus paves the way to efficient production in microbial cell factories.

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Section: Figurementioning

confidence: 99%

Microbial Cell Factories for Tetrahydroisoquinoline Alkaloid Production

2020

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“…Yeasts are extensively exploited for research and industrial bioprocesses for a long time. Among them, S. cerevisiae is non-pathogenic and used for a long time to make alcohol and bread (Mitsui et al, 2019). Copyright © 2021, Journal La Lifesci, Under the license CC BY-SA 4.0…”

Section: Saccharomyces Cerevisiae and Genome Editing Using Crispr-cas9 Systemmentioning

confidence: 99%

“…This system has frequently been used in S. cerevisiae to make efficient knock-outs and knock-ins of genes without a selective marker (Ronda et al, 2015) as well as the addition of multiple genes (Sasaki et al, 2019). It is also used for engineering of metabolic processes such as the addition of pathways or reducing byproduct formation by suppression of multiple gene expressions and improving S. cerevisiae stress tolerance (Shi et al 2016;Mitsui et al 2019).…”

Section: Saccharomyces Cerevisiae and Genome Editing Using Crispr-cas9 Systemmentioning

confidence: 99%

Genome Editing of Saccharomyces Cerevisiae Using CRISPR-Cas9 System

Imran

Ahmed

Muhawesh

2021

JLL

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Saccharomyces cerevisiae is an important yeast has been exploited for a long time to produce alcohol or bread. Moreover, genetically engineered S. cerevisiae cells continue to be used as cell factories for production of biofuels, pharmaceutical proteins and food additives. Genetically modified strain of S. cerevisiae created using traditional methods is laborious and time consuming. Recently, originally an immune system in archaea and bacteria, Clustered regularly interspaced short palindromic repeats “CRISPR” and CRISPR-associated “Cas” have been used exploited as a flexible tool for genome editing. Until now, this tool has been applied to many organisms including yeast. Here, we review the importance of S. cerevisiae as an industrial platform and the use of CRISPR/Cas system and its applications in research and industry of this yeast.

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“…Streptococcus pyogenes type II CRISPR/Cas9-assisted genomic editing and CRISPRi technology are generalizable to microbiologic cell factories, such as C. glutamicum (Cameron Coates et al 2019 ; Cleto et al 2016 ; Liu et al 2017 ) , Escherichia coli (Jiang et al 2015 ; Pyne et al 2015 ), Bacillus subtilis (Altenbuchner 2016 ; Westbrook et al 2016 ), Lactobacillus (Oh and van Pijkeren 2014 ; Song et al 2017 ), Aspergillus (Nodvig et al 2018 ; Zhang et al 2016 ), Saccharomyces cerevisiae (Mitsui et al 2019 ; Zhang et al 2019a ). The CRISPR/Cas9 mutation and CRISPRi (Cleto et al 2016 ; Gauttam et al 2019 ) can fine-tune gene expression level in genome and have been used to study metabolic engineering in C. glutamicum , hence, some strategies for C. glutamicum genomic editing had been documented (Cho et al 2017 ; Peng et al 2017 ; Wang et al 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Construction and application of a CRISPR/Cas9-assisted genomic editing system for Corynebacterium glutamicum

Yao

Wang

2021

AMB Expr

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Corynebacterium glutamicum is widely used as microbial cell factory for various bioproducts, but its genomic editing efficiency needs to be improved. In this study, a highly efficient CRISPR/Cas9-assisted genomic editing system for C. glutamicum was constructed. This system mainly involves a plasmid and can be used for both gene insertion and deletion in the chromosome of C. glutamicum. The recombinant plasmid for the target gene containing all the editing elements, and first constructed it in E. coli, then purified and transformed it into C. glutamicum. This temperature-sensitive plasmid was cured at high temperature after the genomic editing was completed in C. glutamicum. Using this genetic editing system, the genetic editing efficiency in C. glutamicum ATCC 13032 could reach 95%. The whole work of editing could be done in 8–9 days and showed most time-saving compared to the reported. Using this system, the native promoter of gdhA1 in ATCC 13032 has been replaced with the strong promoter PtacM, and more than 10 genes in ATCC 13032 have been deleted. The results demonstrate that this CRISPR/Cas9-assisted system is highly efficient and very suitable for genome editing in C. glutamicum.

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CRISPR system in the yeast Saccharomyces cerevisiae and its application in the bioproduction of useful chemicals

Cited by 25 publications

References 68 publications

Microbial Cell Factories for Tetrahydroisoquinoline Alkaloid Production

Microbial Cell Factories for Tetrahydroisoquinoline Alkaloid Production

Genome Editing of Saccharomyces Cerevisiae Using CRISPR-Cas9 System

Construction and application of a CRISPR/Cas9-assisted genomic editing system for Corynebacterium glutamicum

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