CRISPR Screening Uncovers a Long-Range Enhancer forONECUT1in Pancreatic Differentiation and Links a Diabetes Risk Variant
Samuel Joseph Kaplan,
Wilfred Wong,
Jielin Yan
et al.
Abstract:Functional enhancer annotation is a valuable first step for understanding tissue-specific transcriptional regulation and prioritizing disease-associated non-coding variants for investigation. However, unbiased enhancer discovery in physiologically relevant contexts remains a major challenge. To discover regulatory elements pertinent to diabetes, we conducted a CRISPR interference (CRISPRi) screen in the human pluripotent stem cell (hPSC) pancreatic differentiation system. Among the enhancers uncovered, we focu… Show more
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