CRISPR-Mediated Gene Editing: Scientific and Ethical Issues

Recent Updates of the CRISPR/Cas9 Genome Editing System: Novel Approaches to Regulate Its Spatiotemporal Control by Genetic and Physicochemical Strategies

“…Because of this, there are moral, ethical, and safety issues with the appealing applications of this strategy. 194 Human safety and dignity are at risk, and the possibility of genocide has been brought up by human germline modification via CRISPR/Cas9-based gene editing. A move was made to put a stop to research on the human genome until a decision was made at the national or international level regarding how society would embrace this new technology.…”

New insights on CRISPR/Cas9-based therapy for breast Cancer

“…CRISPR/Cas gene editing technologies have emerged as powerful tools in the study of oncogenic transformation [114]. Although its benefits, it can raise persistent ethical concerns [115]. Recently, CRISPR/Cas9 has been picked as favored technique for genome editing because of its high level of straightforwardness along with the requirements of minimal efforts.…”

“…One of the main drawbacks of this technology is the Off-target effects that might have several pathogenic consequences [115]. On the other hand, on-targets may also result in a wide range of deletions and genomic rearrangements [117].…”

Strategies to overcome the main challenges of the use of CRISPR/Cas9 as a replacement for cancer therapy

“…Non-reproductive cell genome modifications are not heritable, whereas germ cell modifications can be passed down to the next generation. As a result, the attractive uses of this approach raise ethical, moral, and safety concerns [ 213 ]. Human germline modification using CRISPR/Cas9-based gene editing has raised concerns about threats to human safety and dignity, as well as the potential for genocide.…”