CRISPR loss of function screening to identify genes involved in human primordial germ cell-like cell development
Young Sun Hwang,
Yasunari Seita,
M. Andrés Blanco
et al.
Abstract:Despite our increasing knowledge of molecular mechanisms guiding various aspects of human reproduction, those underlying human primordial germ cell (PGC) development remain largely unknown. Here, we conducted custom CRISPR screening in an in vitro system of human PGC-like cells (hPGCLCs) to identify genes required for acquisition and maintenance of PGC fate. Amongst our candidates, we identified TCL1A, an AKT coactivator. Functional assessment in our in vitro hPGCLCs system revealed that TCL1A played a critica… Show more
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