CRISPR-knockout of β-kafirin in sorghum does not recapitulate the grain quality of natural mutants

Massel, Karen; Hintzsche, Jessica; Restall, Jemma; Kerr, Edward D.; Schulz, Benjamin L.; Godwin, Ian D.

doi:10.1007/s00425-022-04038-3

Cited by 8 publications

(2 citation statements)

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“…Non-alpha kafirins, such as the gamma and beta-kafirin, are rich in cysteine ( Belton et al., 2006 ). Recently, Massel et al. (2023) demonstrated that editing the beta-kafirin resulted in an increase of the gamma-kafirin ( Massel et al., 2023 ).…”

Section: Discussionmentioning

confidence: 99%

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Large and stable genome edits at the sorghum alpha kafirin locus result in changes in chromatin accessibility and globally increased expression of genes encoding lysine enrichment

Hurst

Yobi²,

Li³

et al. 2023

Front. Plant Sci.

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IntroductionSorghum is a resilient and widely cultivated grain crop used for feed and food. However, it’s grain is deficient in lysine, an essential amino acid. This is due to the primary seed storage proteins, the alpha-kafirins, lacking lysine. It has been observed that reductions in alpha-kafirin protein results in rebalancing of the seed proteome and a corresponding increase in non-kafirin proteins which leads to an increased lysine content. However, the mechanisms underlying proteome rebalancing are unclear. This study characterizes a previously developed gene edited sorghum line, with deletions at the alpha kafirin locus.MethodsA single consensus guide RNA leads to tandem deletion of multiple members of the gene family in addition to the small target site mutations in remaining genes. RNA-seq and ATAC-seq were utilized to identify changes in gene expression and chromatin accessibility in developing kernels in the absence of most alpha-kafirin expression. ResultsSeveral differentially accessible chromatin regions and differentially expressed genes were identified. Additionally, several genes upregulated in the edited sorghum line were common with their syntenic orthologues differentially expressed in maize prolamin mutants. ATAC-seq showed enrichment of the binding motif for ZmOPAQUE 11, perhaps indicating the transcription factor’s involvement in the kernel response to reduced prolamins.DiscussionOverall, this study provides a resource of genes and chromosomal regions which may be involved in sorghum’s response to reduced seed storage proteins and the process of proteome rebalancing.

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Section: Discussionmentioning

confidence: 99%

“…Recently, Massel et al. (2023) demonstrated that editing the beta-kafirin resulted in an increase of the gamma-kafirin ( Massel et al., 2023 ). If a similar process occurs when editing the alpha-kafirins, rebalancing of the cysteine rich non-alpha kafirins could increase demand for sulfur.…”

Section: Discussionmentioning

confidence: 99%