CRISPR Gene Editing in Strawberry

Genome editing (GE) technology has emerged as a multifaceted strategy that instantaneously popularised the mechanism to modify the genetic constitution of an organism. The clustered regularly interspaced short palindromic repeat (CRISPR) and CRISPR-associated (Cas) protein-based genome editing (CRISPR/Cas) approach has huge potential for efficacious editing of genomes of numerous organisms. This framework has demonstrated to be more economical in contrast to mega-nucleases, zinc-finger nucleases (ZFNs), and transcription activator-like effector nucleases (TALENs) for its flexibility, versatility, and potency. The advent of sequence-specific nucleases (SSNs) allowed the precise induction of double-strand breaks (DSBs) into the genome, ensuring desired alterations through non-homologous end-joining (NHEJ) or homology-directed repair (HDR) pathways. Researchers have utilized CRISPR/Cas-mediated genome alterations across crop varieties to generate desirable characteristics for yield enhancement, enriched nutritional quality, and stressresistance. Here, we highlighted the recent progress in the area of nutritional improvement of crops via the CRISPR/Cas-based tools for fundamental plant research and crop genetic advancements. Application of this genome editing aids in unraveling the basic biology facts in plants supplemented by the incorporation of genome-wide association studies, artificial intelligence, and various bioinformatic frameworks, thereby providing futuristic model studies and their affirmations. Strategies for reducing the 'off-target' effects and the societal approval of genome-modified crops developed via this modern biotechnological approach have been reviewed.

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Revisiting CRISPR/Cas-mediated crop improvement: Special focus on nutrition

Kaul

Sony

Verma

et al. 2020

J Biosci

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Endotoxin contamination in commercially available Cas9 proteins potentially induces T-cell mediated responses

2021

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CRISPR Gene Editing in Strawberry

Cited by 2 publications

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Revisiting CRISPR/Cas-mediated crop improvement: Special focus on nutrition

Revisiting CRISPR/Cas-mediated crop improvement: Special focus on nutrition

Endotoxin contamination in commercially available Cas9 proteins potentially induces T-cell mediated responses

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