CRISPR DNA- and RNP-Mediated Genome Editing via Nicotiana benthamiana Protoplast Transformation and Regeneration

Banakar, Raviraj; Mohan, Krishan; Zhang, Feng

doi:10.1007/978-1-0716-2164-6_5

Cited by 4 publications

(1 citation statement)

References 36 publications

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“…Furthermore, CRISPR-RNP gene editing systems have been established in a range of crops. Including tobacco (Banakar et al 2022a), chili peppers (Kim et al 2020), rice (Banakar et al 2020), tomatoes (Slaman et al 2023), maize (Dong et al 2021), and European chestnut (Pavese et al 2022). In the future, the CRISPR-RNP gene editing system is expected to be widely employed in the genetic modification of crops to enhance adaptability to environmental stresses.…”

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confidence: 99%

Genome editing based on <i>in vitro</i>-assembled ribonucleoproteins in plants

Liang,

Sato,

Matsunaga

2023

CYTOLOGIA

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Gene editing technology based on the clustered regularly interspaced short palindromic repeats (CRISPR) system has garnered widespread use in plant genomes because of its cost-effectiveness, efficiency, and simplicity. To avoid the integration of foreign genes and any DNA fragments into target cell genomes, researchers have developed a system that introduces in vitro-assembled ribonucleoproteins (RNPs) consisting of guide RNA (gRNA) and Cas protein into target cells, enabling direct genome editing. This system was designed to deliver RNPs through four distinct methods: polyethylene glycol (PEG)-mediated cell transfection, particle bombardment, electroporation, and lipid transfection. In recent years, CRISPR technology has been extensively applied for the genetic modification of plants, providing a strategic response to environmental challenges. Researchers have successfully established RNP genome editing systems in various plant species. Despite some remaining issues, the RNP genome editing system still shows significant promise for future applications in the production of non-genetically modified (non-GM) crops.

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mentioning

confidence: 99%

Genome editing based on <i>in vitro</i>-assembled ribonucleoproteins in plants

Liang,

Sato,

Matsunaga

2023

CYTOLOGIA

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Opportunities for Gene Editing of Sweetpotato

Huang,

Livengood,

Yencho

et al. 2024

Compendium of Plant Genomes

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Sweetpotato plays significant roles in the food supply worldwide. Conventional sweetpotato breeding methods face challenges such as self- and cross-incompatibility and high heterogeneity. Gene editing is an effective and powerful tool for modifying agronomic traits, offering a novel approach to develop cultivars by targeting specific genes for precise modifications. The transformed CRISPR/Cas can be segregated out from the gene-edited end product of sexually propagated crops but not in sweetpotato as sweetpotato is highly heterogeneous and has to be propagated clonally. Thus, innovative sweetpotato breeding methods need to be further developed to improve breeding efficacy and decrease breeding cycle. In the present book chapter, we reviewed the methods used for sweetpotato breeding, the success of gene editing in sweetpotato, and the challenges and constraints and the future perspectives of sweetpotato gene editing.

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