CRISPR-Cas9 System for Plant Genome Editing: Current Approaches and Emerging Developments

Montecillo, Jake Adolf V.; Chu, Luan Luong; Bae, Hyeun‐Jong

doi:10.3390/agronomy10071033

Cited by 63 publications

(35 citation statements)

References 216 publications

(320 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The most important and challenging step after the successful delivery of CRISPR/cas9 cassette is the (a) selection of transformants with mutations and (b) the elimination of CRISPR–cas9 cassette, as the presence of it in transformations can increase the rate of off targeting [ 126 , 145 , 146 ]. However, by selfing and back crossing CRISPR-cas9 cassette can be eliminated in T 2 generation and Cas9 free mutants can be produced.…”

Section: Methods For Detection Of Mutation/edits Created By Crispr/cas9mentioning

confidence: 99%

CRISPR-Based Genome Editing Tools: Insights into Technological Breakthroughs and Future Challenges

Mushtaq

Dar

Skalický

et al. 2021

Genes

View full text Add to dashboard Cite

Genome-editing (GE) is having a tremendous influence around the globe in the life science community. Among its versatile uses, the desired modifications of genes, and more importantly the transgene (DNA)-free approach to develop genetically modified organism (GMO), are of special interest. The recent and rapid developments in genome-editing technology have given rise to hopes to achieve global food security in a sustainable manner. We here discuss recent developments in CRISPR-based genome-editing tools for crop improvement concerning adaptation, opportunities, and challenges. Some of the notable advances highlighted here include the development of transgene (DNA)-free genome plants, the availability of compatible nucleases, and the development of safe and effective CRISPR delivery vehicles for plant genome editing, multi-gene targeting and complex genome editing, base editing and prime editing to achieve more complex genetic engineering. Additionally, new avenues that facilitate fine-tuning plant gene regulation have also been addressed. In spite of the tremendous potential of CRISPR and other gene editing tools, major challenges remain. Some of the challenges are related to the practical advances required for the efficient delivery of CRISPR reagents and for precision genome editing, while others come from government policies and public acceptance. This review will therefore be helpful to gain insights into technological advances, its applications, and future challenges for crop improvement.

show abstract

Section: Methods For Detection Of Mutation/edits Created By Crispr/cas9mentioning

confidence: 99%

CRISPR-Based Genome Editing Tools: Insights into Technological Breakthroughs and Future Challenges

Mushtaq

Dar

Skalický

et al. 2021

Genes

View full text Add to dashboard Cite

show abstract

“…These may include a low mutation efficiency/rate, unwanted (off-target) mutations, inefficient gene delivery techniques, in vitro regeneration dependency, persistent activity in subsequent generations, the spread of transgene to other plants and reversion of mutation via cross-pollination, difficulties of execution in woody plants and long-life cycle, genotypic chimerism and strict GMO regulations. Most of these issues can be tackled through a meticulous approach by avoiding erroneous gRNA design, choosing the best Cas protein variant, designing a better expression cassette (including a promoter), employing a highly efficient DNA delivery approach and targeting the right tissue types [ 245 , 246 , 247 ].…”

Section: Critical Assessment Of Crispr-cas Based Ge In Fruit Treesmentioning

confidence: 99%

Induced Genetic Variations in Fruit Trees Using New Breeding Tools: Food Security and Climate Resilience

Sattar

Iqbal

Al-Khayri

et al. 2021

Plants

View full text Add to dashboard Cite

Fruit trees provide essential nutrients to humans by contributing to major agricultural outputs and economic growth globally. However, major constraints to sustainable agricultural productivity are the uncontrolled proliferation of the population, and biotic and abiotic stresses. Tree mutation breeding has been substantially improved using different physical and chemical mutagens. Nonetheless, tree plant breeding has certain crucial bottlenecks including a long life cycle, ploidy level, occurrence of sequence polymorphisms, nature of parthenocarpic fruit development and linkage. Genetic engineering of trees has focused on boosting quality traits such as productivity, wood quality, and resistance to biotic and abiotic stresses. Recent technological advances in genome editing provide a unique opportunity for the genetic improvement of woody plants. This review examines application of the CRISPR-Cas system to reduce disease susceptibility, alter plant architecture, enhance fruit quality, and improve yields. Examples are discussed of the contemporary CRISPR-Cas system to engineer easily scorable PDS genes, modify lignin, and to alter the flowering onset, fertility, tree architecture and certain biotic stresses.

show abstract

“…The selection of the promoter for the expression of Cas9 and sgRNAs and the configuration of these expression cassettes are crucial to achieve proficient genome editing. Commonly, there are three expression strategies for the CRISPR-Cas system in plants, i.e., a mixed dual promoter system, a dual Pol II promoter system, and a single transcriptional unit system (STU) [232]. Although the STU system is considered superior over other two systems, it has limitations i.e., refinement of the system, nonoptimal expression system, and difficult post-transcriptional processes [232].…”

Section: Conclusion and Future Prospectsmentioning

confidence: 99%

“…Commonly, there are three expression strategies for the CRISPR-Cas system in plants, i.e., a mixed dual promoter system, a dual Pol II promoter system, and a single transcriptional unit system (STU) [232]. Although the STU system is considered superior over other two systems, it has limitations i.e., refinement of the system, nonoptimal expression system, and difficult post-transcriptional processes [232]. The limitations could be combatted by using a bidirectional promoter system that can initiate transcription in both orientations [233].…”

Section: Conclusion and Future Prospectsmentioning

confidence: 99%

RNA Interference and CRISPR/Cas Gene Editing for Crop Improvement: Paradigm Shift towards Sustainable Agriculture

Rajput

Choudhary

Kumar

et al. 2021

Plants

View full text Add to dashboard Cite

With the rapid population growth, there is an urgent need for innovative crop improvement approaches to meet the increasing demand for food. Classical crop improvement approaches involve, however, a backbreaking process that cannot equipoise with increasing crop demand. RNA-based approaches i.e., RNAi-mediated gene regulation and the site-specific nuclease-based CRISPR/Cas9 system for gene editing has made advances in the efficient targeted modification in many crops for the higher yield and resistance to diseases and different stresses. In functional genomics, RNA interference (RNAi) is a propitious gene regulatory approach that plays a significant role in crop improvement by permitting the downregulation of gene expression by small molecules of interfering RNA without affecting the expression of other genes. Gene editing technologies viz. the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein (CRISPR/Cas) have appeared prominently as a powerful tool for precise targeted modification of nearly all crops’ genome sequences to generate variation and accelerate breeding efforts. In this regard, the review highlights the diverse roles and applications of RNAi and CRISPR/Cas9 system as powerful technologies to improve agronomically important plants to enhance crop yields and increase tolerance to environmental stress (biotic or abiotic). Ultimately, these technologies can prove to be important in view of global food security and sustainable agriculture.

show abstract

CRISPR-Cas9 System for Plant Genome Editing: Current Approaches and Emerging Developments

Cited by 63 publications

References 216 publications

CRISPR-Based Genome Editing Tools: Insights into Technological Breakthroughs and Future Challenges

CRISPR-Based Genome Editing Tools: Insights into Technological Breakthroughs and Future Challenges

Induced Genetic Variations in Fruit Trees Using New Breeding Tools: Food Security and Climate Resilience

RNA Interference and CRISPR/Cas Gene Editing for Crop Improvement: Paradigm Shift towards Sustainable Agriculture

Contact Info

Product

Resources

About